The paper was supported by The Second Land Investigation Item and took Nanjing city, Jiangsu Province as a case study. The research of the theory, technique and application for land use investigation was achieved by the high-resolution remote sensing images for application, designed a set of technique of land use investigation for land property right management. The database and platform system were established to carry out the dynamic management of land use. Based on the summarization of the correlative studies, The paper designed a set of technique of land investigation for land property right management and also designed the technical process, dealt with the remote sensing images, detected the changed information, classified the land, investigated the land property right and established the database to serve for the management of land property right. And it has been successfully used in Nanjing. It’s unique to use the high-resolution remote sensing images by QuichBird for the scale of 1:5000 in land use investigation in area cities which is also the first time in Nanjing City.