This paper proposes a novel microfluidic system for cell/microparticle recognition and
manipulation utilizing digital image processing technique (DIP) and optical tweezer under
microfluidic configuration. Digital image processing technique is used to count and recognize the
cell/particle samples and then sends a control signal to generate a laser pulse to manipulate the target
cell/particle optically. The optical tweezer system is capable of catching, moving and switching the
target cells at the downstream of the microchannel. The trapping force of the optical tweezer is also
demonstrated utilizing Stocks-drag method and electroosmotic flow. The proposed system provides a
simple but high-performance solution for microparticle manipulation in a microfluidic device.