Layer-oriented Integration Vector Element of Multilayer RAM

2008 ◽  
Author(s):  
Yongjie Zhang ◽  
Dong Nie ◽  
Qin Sun
Keyword(s):  
Integration Vector ◽  
Vector Element

scholarly journals Construction of an Integration-Proficient Vector Based on the Site-Specific Recombination Mechanism of Enterococcal Temperate Phage φFC1

2002 ◽  
Vol 184 (7) ◽  
pp. 1859-1864 ◽  
Author(s):  
Hee-Youn Yang ◽  
Young-Woo Kim ◽  
Hyo-Ihl Chang
Keyword(s):  
Pcr Amplification ◽  
Temperate Phage ◽  
Vector System ◽  
Recombination Mechanism ◽  
Integrase Gene ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Integration Vector ◽  
Site Specific Integration ◽  
A Site

ABSTRACT The genome of temperate phage φFC1 integrates into the chromosome of Enterococcus faecalis KBL 703 via site-specific recombination. In this study, an integration vector containing the attP site and putative integrase gene mj1 of phage φFC1 was constructed. A 2,744-bp fragment which included the attP site and mj1 was inserted into a pUC19 derivative containing the cat gene to construct pEMJ1-1. E. faecalis KBL 707, which does not contain the bacteriophage but which has a putative attB site within its genome, could be transformed by pEMJ1-1. Southern hybridization, PCR amplification, and DNA sequencing revealed that pEMJ1-1 was integrated specifically at the putative attB site within the E. faecalis KBL 707 chromosome. This observation suggested that the 2,744-bp fragment carrying mj1 and the attP site of phage φFC1 was sufficient for site-specific recombination and that pEMJ1-1 could be used as a site-specific integration vector. The transformation efficiency of pEMJ1-1 was as high as 6 × 103 transformants/μg of DNA. In addition, a vector (pATTB1) containing the 290-bp attB region was constructed. pATTB1 was transformed into Escherichia coli containing a derivative of the pET14b vector carrying attP and mj1. This resulted in the formation of chimeric plasmids by site-specific recombination between the cloned attB and attP sequences. The results indicate that the integration vector system based on the site-specific recombination mechanism of phage φFC1 can be used for genetic engineering in E. faecalis and in other hosts.

A xylose-inducible Bacillus subtilis integration vector and its application

Gene ◽  
1996 ◽  
Vol 181 (1-2) ◽  
pp. 71-76 ◽  
Author(s):  
Lana Kim ◽  
Axel Mogk ◽  
Wolfgang Schumann
Keyword(s):  
Bacillus Subtilis ◽  
Integration Vector

scholarly journals Construction of a single-copy integration vector and its use to study gene expression in Bacillus licheniformis

Microbiology ◽  
1998 ◽  
Vol 144 (9) ◽  
pp. 2573-2578 ◽  
Author(s):  
L.-S. P. Tran ◽  
L. Szabo ◽  
L. Orosz ◽  
T. Sik ◽  
A. Holczinger
Keyword(s):  
Gene Expression ◽  
Bacillus Licheniformis ◽  
Single Copy ◽  
Integration Vector ◽  
Study Gene Expression

scholarly journals A novel vector element providing multicopy vector integration inArxula adeninivorans

2007 ◽  
Vol 7 (7) ◽  
pp. 1197-1205 ◽  
Author(s):  
Gerhard Steinborn ◽  
Gerd Gellissen ◽  
Gotthard Kunze
Keyword(s):  
Vector Integration ◽  
Multicopy Vector ◽  
Vector Element

scholarly journals A Domain Decomposition Method for Hybrid Shell Vector Element with Boundary Integral Method

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lin Lei ◽  
Jun Hu ◽  
Hao-Quan Hu
Keyword(s):  
Domain Decomposition ◽  
Domain Decomposition Method ◽  
Three Dimensional ◽  
Shell Element ◽  
Boundary Integral ◽  
Boundary Integral Method ◽  
Thin Coating ◽  
Surface Integral ◽  
Vector Element ◽  
Element Method

For the conducting body coated with thin-layer material, plenty of fine meshes are required in general. In this paper, shell vector element (SVE) is used for modeling of thin coating dielectric. Further, a domain decomposition (DD) method for hybrid shell vector element method boundary integral (SVE-BI) is proposed for analysis of electromagnetic problem of multiple three-dimensional thin-coating objects. By this method, the whole computational domains are divided into sub-SVE domains and boundary element domains. With shell element, not only the unknowns are far less than the one by traditional vector element method, but only surface integral is required. The DDM framework used for hybrid SVE-BI also enhances the computational efficiency of solving scattering from multiple coating objects greatly. Finally, several numerical examples are presented to prove the accuracy and efficiency of this DDM-SVE-BI method.

scholarly journals Diacylglycerol Kinase Is Involved in Regulation of Expression of the Lantibiotic Mutacin II of Streptococcus mutans

1998 ◽  
Vol 180 (1) ◽  
pp. 167-170 ◽  
Author(s):  
Ping Chen ◽  
Jan Novak ◽  
Fengxia Qi ◽  
Page W. Caufield
Keyword(s):  
Environmental Stress ◽  
Gene Product ◽  
Streptococcus Mutans ◽  
Structural Gene ◽  
Genetic Characterization ◽  
Diacylglycerol Kinase ◽  
Primer Extension ◽  
Regulation Of Expression ◽  
Integration Vector

ABSTRACT Genetic characterization of a Tn916 transposon mutant,Streptococcus mutans T8-1, defective in mutacin II production, revealed that the transposon was inserted into the 3′ region of a diacylglycerol kinase (dgk) gene. The insertion occurred in the same region as described for another S. mutans mutant, GS5Tn1, which was altered in its ability to respond to environmental stress (Y. Yamashita, T. Takehara, and H. K. Kuramitsu, J. Bacteriol. 175:6220–6228, 1993). Quantitative primer extension from the mutacin structural gene mutA showed a decreased level (about eightfold) of mutA transcription for mutant T8-1. Mutacin production was restored by transforming mutant T8-1 with integration vector pVA891 containing an intactdgk gene. These data indicated that the full-lengthdgk gene product along with the mutacin biosynthetic operon are required for the production of the mutacin II lantibiotic.

scholarly journals Sobre um experimento na educação de jovens e adultos: a aprendizagem por meio da construção de fotonovela

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Rodrigo Sychocki Silva ◽  
Carla Angélica Ludwig
Keyword(s):  
Adult Education ◽  
Soap Opera ◽  
Porto Alegre ◽  
Teaching Methodologies ◽  
Guiding Principle ◽  
The Subject ◽  
Educational Debate ◽  
Vector Element

Resumo: O presente trabalho versa sobre o planejamento e execução de uma prática pedagógica envolvendo ludicidade na Educação de Jovens e Adultos (EJA). Via de regra, a ludicidade está relacionada com a educação de crianças. O estudo problematiza tal relação, ao pontuar o lúdico como elemento vetorial na aprendizagem e constituição do sujeito, independente da faixa etária. Dentre as experiências realizadas em uma escola da rede municipal de Porto Alegre, a construção da fotonovela intitulada “Maria Esperança” oportunizou utilizar a ludicidade como princípio orientador. A partir das análises e reflexões sobre o projeto pretende-se contribuir no debate educacional que envolve a EJA e suas consequências na formação dos estudantes. Com a pesquisa foi possível inferir que realizar atividades lúdicas com turmas de EJA, sem recair na infantilização torna-se um recurso a ser utilizado em sala de aula, desde que se considere o contexto no qual está inserido o estudante, suas potencialidades e subjetividades. Palavras-chave: Aprendizagem. Educação de Jovens e Adultos. Metodologias de Ensino. Lúdico. ON AN EXPERIMENT IN YOUTH AND ADULT EDUCATION: THROUGH LEARNING PHOTO SOAP OPERA CONSTRUCTION Abstract: This paper deals with the planning and execution of a pedagogic practice involving playfulness in the Youth and Adult Education (EJA). As a rule, the playfulness is related to the education of children. The study discusses this relationship by pointing playful as a vector element in learning and constitution of the subject, regardless of age. Among the experiments in a municipal school in Porto Alegre, the construction of photo soap opera entitled "Mary Hope" provided an opportunity to use playfulness as a guiding principle. Based on the analysis and reflections about the project, there is the intention to contribute to the educational debate involving the EJA and its consequences in the training of students. Through this research it was possible to infer that performing fun activities with adult education classes, without falling into infantilization becomes a resource to be used in the classroom, since it considers the context in which the student is inserted, their potential and subjectivities. Keywords: Learning. Youth and Adult Education. Teaching methodologies. Playful. 

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