High‐throughput sequencing‐based microsatellite genotyping for polyploids to resolve allele dosage uncertainty and improve analyses of genetic diversity, structure and differentiation: A case study of the hexaploid Camellia oleifera

2021 ◽  
Author(s):  
Xiangyan Cui ◽  
Caihua Li ◽  
Shengyuan Qin ◽  
Zebin Huang ◽  
Bin Gan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Camellia Oleifera ◽  
Microsatellite Genotyping ◽  
Allele Dosage ◽  
Diversity Structure

scholarly journals Modelling the Evolutionary Dynamics of Viruses within Their Hosts: A Case Study Using High-Throughput Sequencing

2012 ◽  
Vol 8 (4) ◽  
pp. e1002654 ◽  
Author(s):  
Frédéric Fabre ◽  
Josselin Montarry ◽  
Jérôme Coville ◽  
Rachid Senoussi ◽  
Vincent Simon ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Evolutionary Dynamics

scholarly journals High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 385 ◽  
Author(s):  
Asimina Katsiani ◽  
Varvara Maliogka ◽  
Nikolaos Katis ◽  
Laurence Svanella-Dumas ◽  
Antonio Olmos ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sour Cherry ◽  
Prunus Species ◽  
Cherry Tree ◽  
High Genetic Diversity ◽  
Viral Genomes ◽  
Virus Diversity ◽  
First Time

Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics.

scholarly journals SNP identification and validation on genomic DNA for studying genetic diversity in Thunnus albacares and Scomberomorus brasiliensis by combining RADseq and long read high throughput sequencing

2018 ◽  
Vol 198 ◽  
pp. 189-194 ◽  
Author(s):  
Zoila Raquel Siccha-Ramirez ◽  
Francesco Maroso ◽  
Belén G. Pardo ◽  
Carlos Fernández ◽  
Paulino Martínez ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
High Throughput ◽  
Genomic Dna ◽  
High Throughput Sequencing ◽  
Thunnus Albacares ◽  
Long Read

High-throughput sequencing analysis reveals the genetic diversity of different regions of the murine norovirus genome during in vitro replication

2016 ◽  
Vol 162 (4) ◽  
pp. 1019-1023 ◽  
Author(s):  
Axel Mauroy ◽  
Bernard Taminiau ◽  
Carine Nezer ◽  
Elsa Ghurburrun ◽  
Denis Baurain ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sequencing Analysis ◽  
Murine Norovirus ◽  
In Vitro Replication
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