scholarly journals Characterizing the spatial signal of environmental DNA in river systems using a community ecology approach

Author(s):  
Isabel Cantera ◽  
Jean‐Baptiste Decotte ◽  
Tony Dejean ◽  
Jérôme Murienne ◽  
Régis Vigouroux ◽  
...  
2020 ◽  
Author(s):  
Isabel Cantera ◽  
Jean-Baptiste Decotte ◽  
Tony Dejean ◽  
Jérôme Murienne ◽  
Régis Vigouroux ◽  
...  

AbstractEnvironmental DNA (eDNA) is gaining a growing popularity among scientists but its applicability to biodiversity research and management remain limited in river systems by the lack of knowledge about the spatial extent of the downstream transport of eDNA. Up to now, attempts to measure eDNA detection distance compared known species distributions to eDNA results, limiting therefore studies to a few intensively studied rivers. Here we developed a framework to measure the detection distance of eDNA in rivers based on the comparison of faunas across an increasing range of spatial extents, making it independent from knowledge on species distributions. We hypothesized that under short detection distance the similarity between fish faunas should peak between nearby sites, whereas under long detection distance each site should cumulate species from a large upstream area. Applying this framework to the fish fauna of two large and species rich Neotropical river basins (Maroni and Oyapock), we show that fish eDNA detection distance did not exceed a few kilometers. eDNA hence provided inventories of local species communities. Those results were validated by retrieving the distance decay of species similarity, a general pattern in ecology based on the decline of local species community similarity with spatial distance between them. We finally compared species distribution derived from eDNA to the known distribution of the species based on capture data, and this comparison also confirmed a global match between methods, testifying for a short distance of detection of the fauna by eDNA.


2020 ◽  
Vol 123 (4) ◽  
pp. 655-668
Author(s):  
N. Lenhardt ◽  
W. Altermann ◽  
F. Humbert ◽  
M. de Kock

Abstract The Palaeoproterozoic Hekpoort Formation of the Pretoria Group is a lava-dominated unit that has a basin-wide extent throughout the Transvaal sub-basin of South Africa. Additional correlative units may be present in the Kanye sub-basin of Botswana. The key characteristic of the formation is its general geochemical uniformity. Volcaniclastic and other sedimentary rocks are relatively rare throughout the succession but may be dominant in some locations. Hekpoort Formation outcrops are sporadic throughout the basin and mostly occur in the form of gentle hills and valleys, mainly encircling Archaean domes and the Palaeoproterozoic Bushveld Complex (BC). The unit is exposed in the western Pretoria Group basin, sitting unconformably either on the Timeball Hill Formation or Boshoek Formation, which is lenticular there, and on top of the Boshoek Formation in the east of the basin. The unit is unconformably overlain by the Dwaalheuwel Formation. The type-locality for the Hekpoort Formation is the Hekpoort farm (504 IQ Hekpoort), ca. 60 km to the west-southwest of Pretoria. However, no stratotype has ever been proposed. A lectostratotype, i.e., the Mooikloof area in Pretoria East, that can be enhanced by two reference stratotypes are proposed herein. The Hekpoort Formation was deposited in a cratonic subaerial setting, forming a large igneous province (LIP) in which short-termed localised ponds and small braided river systems existed. It therefore forms one of the major Palaeoproterozoic magmatic events on the Kaapvaal Craton.


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