In situ measurements of animal morphological features: A non‐invasive method

2017 ◽  
Vol 9 (3) ◽  
pp. 613-623 ◽  
Author(s):  
Mylswamy Mahendiran ◽  
Mylswamy Parthiban ◽  
Parappurath Abdul Azeez ◽  
Rajarathinavelu Nagarajan
Keyword(s):  
In Situ Measurements ◽  
Morphological Features ◽  
Non Invasive ◽  
Invasive Method

scholarly journals Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

10.3791/60447 ◽  
2019 ◽  
Author(s):  
Klemens Weisleitner ◽  
Lars Hunger ◽  
Christoph Kohstall ◽  
Albert Frisch ◽  
Michael C. Storrie-Lombardi ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Laser Induced Fluorescence ◽  
In Situ Measurements ◽  
Non Invasive

scholarly journals In situCharacterisation of Living Cells by Raman Spectroscopy

2002 ◽  
Vol 16 (2) ◽  
pp. 43-51 ◽  
Author(s):  
I. Notingher ◽  
S. Verrier ◽  
H. Romanska ◽  
A. E. Bishop ◽  
J. M. Polak ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Laser Excitation ◽  
Living Cells ◽  
Fused Silica ◽  
Excitation Wavelength ◽  
Bioactive Scaffolds ◽  
Non Invasive ◽  
Invasive Method ◽  
45S5 Bioglass

We report the first Raman spectra of individual living and dead cells (MLE-12 line) cultured on bioinert standard poly-L-lysine coated fused silica and on bioactive 45S5 Bioglass®measured at 785 nm laser excitation. At this excitation wavelength no damage was induced to the cells even after 40 minutes irradiation at 115 mW power, as indicated by cell morphology observation and trypan blue viability test. We show that shorter wavelength lasers, 488 nm and 514 nm, cannot be used because they induce damage to the cells at very low laser powers (5 mW) and short irradiation times (5–20 minutes). The most important differences between the spectra of living and dead cells are in the 1530–1700 cm−1range, where the dead cells have strong peaks at 1578 cm−1and 1607 cm−1. Other differences occur around the DNA peak at 1094 cm−1. Our study establishes the feasibility of using the 785 nm laser for anin situreal-time non-invasive method to follow biological events (proliferation, differentiation, cell death, etc.) within individual cells cultured on bioactive scaffolds in their physiologic environment over long periods of time.

Locating an ice-covered Antarctic landfill using ground magnetometry

2014 ◽  
Vol 26 (4) ◽  
pp. 361-368 ◽  
Author(s):  
Benjamin L. Freidman ◽  
Danielle Camenzuli ◽  
Mark Lackie
Keyword(s):  
Metal Contamination ◽  
Cost Effective ◽  
Magnetic Anomalies ◽  
Material Surface ◽  
Magnetic Susceptibilities ◽  
North West ◽  
Non Invasive ◽  
Invasive Method ◽  
Antarctic Research

AbstractAt former Antarctic research stations, legacy waste often remains in situ and concealed by ice. Consequently, the location, characteristics and potential environmental impact associated with legacy waste remains poorly documented. This study applies ground magnetometry to map the spatial extent of the landfill at the abandoned Wilkes Station. Magnetic anomalies indicate that the landfill extends north-west to south-east and is close to, and perhaps prograding into, the ocean. The landfill is characterized by large magnetic variations of > 1500 nT with asymmetrical magnetic anomalies which suggest variable orientations of material and random dumping. Magnetic susceptibilities > 0.02SI units beyond the landfill area reveal elevated magnetic properties of the basement geology. However, a contrast in anomaly shape reliably distinguishes large anomalies generated by landfill material. Surface and subsurface melt streams (observed at the shoreline) flowing from the survey area suggest elevated potential for metal contamination of the nearshore and marine environment. The survey demonstrates a cost-effective and non-invasive method for gathering information to guide the clean up of landfills beneath ice.

scholarly journals In situ detection of salicylate in Ocimum basilicum plant leaves via reverse iontophoresis

2015 ◽  
Vol 51 (92) ◽  
pp. 16534-16536 ◽  
Author(s):  
M. I. González-Sánchez ◽  
P. T. Lee ◽  
R. H. Guy ◽  
R. G. Compton
Keyword(s):  
Cyclic Voltammetry ◽  
Quantitative Method ◽  
Ocimum Basilicum ◽  
Plant Leaves ◽  
Reverse Iontophoresis ◽  
Non Invasive ◽  
Screen Printed Electrodes ◽  
Invasive Method ◽  
In Situ Detection

We report an in situ quantitative method to measure the concentration of salicylates, from intact, living Ocimum basilicum plant leaves. This simple, non-invasive method utilises iontophoresis in combination with cyclic voltammetry at disposable screen-printed electrodes.

scholarly journals The importance of continuing research during the demolition of architectural wooden architecture on the example of pens from Kaniczek translocated into 'Olęder' Ethnographic Park in Nieszawka Wielka

2015 ◽  
Vol 14 (3) ◽  
pp. 089-106
Author(s):  
Anna Maślak
Keyword(s):  
Main Task ◽  
Progressive Deterioration ◽  
Non Invasive ◽  
Conservation Project ◽  
Conservation Measures ◽  
Invasive Method ◽  
The Creation ◽  
Facility Construction ◽  
Architectural Research

'Olęder' Ethnographic Park in Nieszawka Wielka is Poland's only open-air museum that has been dedicated to construction of the rural, associated with olęder settlement. Currently, its research range covers an area of Lower Vistula Valley. Existing conservation measures, which main task is to protect architectural monuments in situ, however, is not always sufficient or possible. Preserved until today, very few examples of this construction are threatened by the progressive deterioration leading to their elimination from the Vistula landscape. The creation of a museum in the open air and translocation of these precious relics is actually the only chance for their continued survival. As a preliminary point, I will briefly present the characteristics of the 'olęder' settlement in the Lower Vistula Valley and the concept of 'Olęder' Ethnographic Park in Nieszawka Wielka along with attempts to establish a new branch of the Museum. The purpose of this article is to highlight the importance of supporting architectural research during the demolition of wooden architecture on the example of pens in Kaniczek translocated into 'Olęder' Ethnographic Park in Nieszawka Wielka. Initially, the concept of conservation project was discussed, developed on the basis of architectural research conducted before demolition. These studies were conducted using a non-invasive method, making it difficult to access all components and places. Supplementing the research during the demolition shed a new light on conservation issues. The summary of activities related to the translocation will show the last phase of facility construction.

scholarly journals Application of the TDR measuring technique for in-situ measurements using surface probes

2011 ◽  
Vol 8 (1) ◽  
pp. 097-106
Author(s):  
Zbigniew Suchorab ◽  
Zbyšek Pavlík ◽  
Robert Černý
Keyword(s):  
Time Domain ◽  
Time Domain Reflectometry ◽  
In Situ Measurements ◽  
Measuring Technique ◽  
Ceramic Brick ◽  
Non Invasive

The article presents the possibility of in-situ measurements of building barriers moisture using the TDR technique (Time Domain Reflectometry). To minimize the previous disadvantages of the described method – invasive character, several prototypes of the surface probes were manufactured. With such a sensor solution it was possible to conduct the non-invasive measurements using the full TDR method potential – quick measurements and no sensitivity of salinity influence. The measurements were conducted on the brickworks made of red ceramic brick at the old building located on 1st of May Street in Lublin.

scholarly journals UV-Vis spectroscopy

2018 ◽  
Vol 4 (4) ◽  
Author(s):  
Marcello Picollo ◽  
Maurizio Aceto ◽  
Tatiana Vitorino
Keyword(s):  
Cultural Heritage ◽  
Case Studies ◽  
High Sensitivity ◽  
Reflectance Spectroscopy ◽  
Non Invasive ◽  
Vis Spectroscopy ◽  
Sensitivity And Selectivity ◽  
Invasive Method ◽  
Short Time

Abstract UV-Vis reflectance spectroscopy has been widely used as a non-invasive method for the study of cultural heritage materials for several decades. In particular, FORS, introduced in the 1980s, allows to acquire hundreds of reflectance spectra in situ in a short time, contributing to the identification of artist’s materials. More recently, microspectrofluorimetry has also been proposed as a powerful non-invasive method for the identification of dyes and lake pigments that provides high sensitivity and selectivity. In this chapter, the concepts behind these spectroscopic methodologies will be discussed, as well as the instrumentation and measurement modes used. Case studies related with different cultural heritage materials (paintings and manuscripts, textiles, carpets and tapestries, glass, metals, and minerals), which show the usefulness of UV-Vis reflectance spectroscopy and microspectrofluorimetry applied to the study of artworks, will also be presented.

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