A non invasive method to detect stratigraphy, thicknesses and pigment concentration of pictorial multilayers based on EDXRF and vis-RS: in situ applications

2008 ◽  
Vol 92 (1) ◽  
pp. 203-210 ◽  
Author(s):  
L. Bonizzoni ◽  
S. Caglio ◽  
A. Galli ◽  
G. Poldi
2002 ◽  
Vol 16 (2) ◽  
pp. 43-51 ◽  
Author(s):  
I. Notingher ◽  
S. Verrier ◽  
H. Romanska ◽  
A. E. Bishop ◽  
J. M. Polak ◽  
...  

We report the first Raman spectra of individual living and dead cells (MLE-12 line) cultured on bioinert standard poly-L-lysine coated fused silica and on bioactive 45S5 Bioglass®measured at 785 nm laser excitation. At this excitation wavelength no damage was induced to the cells even after 40 minutes irradiation at 115 mW power, as indicated by cell morphology observation and trypan blue viability test. We show that shorter wavelength lasers, 488 nm and 514 nm, cannot be used because they induce damage to the cells at very low laser powers (5 mW) and short irradiation times (5–20 minutes). The most important differences between the spectra of living and dead cells are in the 1530–1700 cm−1range, where the dead cells have strong peaks at 1578 cm−1and 1607 cm−1. Other differences occur around the DNA peak at 1094 cm−1. Our study establishes the feasibility of using the 785 nm laser for anin situreal-time non-invasive method to follow biological events (proliferation, differentiation, cell death, etc.) within individual cells cultured on bioactive scaffolds in their physiologic environment over long periods of time.


2014 ◽  
Vol 26 (4) ◽  
pp. 361-368 ◽  
Author(s):  
Benjamin L. Freidman ◽  
Danielle Camenzuli ◽  
Mark Lackie

AbstractAt former Antarctic research stations, legacy waste often remains in situ and concealed by ice. Consequently, the location, characteristics and potential environmental impact associated with legacy waste remains poorly documented. This study applies ground magnetometry to map the spatial extent of the landfill at the abandoned Wilkes Station. Magnetic anomalies indicate that the landfill extends north-west to south-east and is close to, and perhaps prograding into, the ocean. The landfill is characterized by large magnetic variations of > 1500 nT with asymmetrical magnetic anomalies which suggest variable orientations of material and random dumping. Magnetic susceptibilities > 0.02SI units beyond the landfill area reveal elevated magnetic properties of the basement geology. However, a contrast in anomaly shape reliably distinguishes large anomalies generated by landfill material. Surface and subsurface melt streams (observed at the shoreline) flowing from the survey area suggest elevated potential for metal contamination of the nearshore and marine environment. The survey demonstrates a cost-effective and non-invasive method for gathering information to guide the clean up of landfills beneath ice.


2015 ◽  
Vol 51 (92) ◽  
pp. 16534-16536 ◽  
Author(s):  
M. I. González-Sánchez ◽  
P. T. Lee ◽  
R. H. Guy ◽  
R. G. Compton

We report an in situ quantitative method to measure the concentration of salicylates, from intact, living Ocimum basilicum plant leaves. This simple, non-invasive method utilises iontophoresis in combination with cyclic voltammetry at disposable screen-printed electrodes.


2015 ◽  
Vol 14 (3) ◽  
pp. 089-106
Author(s):  
Anna Maślak

'Olęder' Ethnographic Park in Nieszawka Wielka is Poland's only open-air museum that has been dedicated to construction of the rural, associated with olęder settlement. Currently, its research range covers an area of Lower Vistula Valley. Existing conservation measures, which main task is to protect architectural monuments in situ, however, is not always sufficient or possible. Preserved until today, very few examples of this construction are threatened by the progressive deterioration leading to their elimination from the Vistula landscape. The creation of a museum in the open air and translocation of these precious relics is actually the only chance for their continued survival. As a preliminary point, I will briefly present the characteristics of the 'olęder' settlement in the Lower Vistula Valley and the concept of 'Olęder' Ethnographic Park in Nieszawka Wielka along with attempts to establish a new branch of the Museum. The purpose of this article is to highlight the importance of supporting architectural research during the demolition of wooden architecture on the example of pens in Kaniczek translocated into 'Olęder' Ethnographic Park in Nieszawka Wielka. Initially, the concept of conservation project was discussed, developed on the basis of architectural research conducted before demolition. These studies were conducted using a non-invasive method, making it difficult to access all components and places. Supplementing the research during the demolition shed a new light on conservation issues. The summary of activities related to the translocation will show the last phase of facility construction.


2017 ◽  
Vol 9 (3) ◽  
pp. 613-623 ◽  
Author(s):  
Mylswamy Mahendiran ◽  
Mylswamy Parthiban ◽  
Parappurath Abdul Azeez ◽  
Rajarathinavelu Nagarajan

2018 ◽  
Vol 4 (4) ◽  
Author(s):  
Marcello Picollo ◽  
Maurizio Aceto ◽  
Tatiana Vitorino

Abstract UV-Vis reflectance spectroscopy has been widely used as a non-invasive method for the study of cultural heritage materials for several decades. In particular, FORS, introduced in the 1980s, allows to acquire hundreds of reflectance spectra in situ in a short time, contributing to the identification of artist’s materials. More recently, microspectrofluorimetry has also been proposed as a powerful non-invasive method for the identification of dyes and lake pigments that provides high sensitivity and selectivity. In this chapter, the concepts behind these spectroscopic methodologies will be discussed, as well as the instrumentation and measurement modes used. Case studies related with different cultural heritage materials (paintings and manuscripts, textiles, carpets and tapestries, glass, metals, and minerals), which show the usefulness of UV-Vis reflectance spectroscopy and microspectrofluorimetry applied to the study of artworks, will also be presented.


2020 ◽  
Author(s):  
Maria Sonsoles Garcia Alonso ◽  
Antonio Hernando ◽  
Jordi Viñolas ◽  
Miguel Angel Garcia

Abstract We present here a non-invasive method to measure variations of internal stress in magnetic steel elements. The method consists in the measurement of the magnetic permeability on the specimen and relays on the dependence of its imaginary component with applied load. Our method does not measure absolute values and hence, results very suitable for measurements in situ, where small changes of positions or vibrations can change those absolute values.


2001 ◽  
Vol 12 (1) ◽  
pp. 8-14
Author(s):  
Gertraud Teuchert-Noodt ◽  
Ralf R. Dawirs

Abstract: Neuroplasticity research in connection with mental disorders has recently bridged the gap between basic neurobiology and applied neuropsychology. A non-invasive method in the gerbil (Meriones unguiculus) - the restricted versus enriched breading and the systemically applied single methamphetamine dose - offers an experimental approach to investigate psychoses. Acts of intervening affirm an activity dependent malfunctional reorganization in the prefrontal cortex and in the hippocampal dentate gyrus and reveal the dopamine position as being critical for the disruption of interactions between the areas concerned. From the extent of plasticity effects the probability and risk of psycho-cognitive development may be derived. Advance may be expected from insights into regulatory mechanisms of neurogenesis in the hippocampal dentate gyrus which is obviously to meet the necessary requirements to promote psycho-cognitive functions/malfunctions via the limbo-prefrontal circuit.


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