Sperm chromatin integrity in a man with macrocephaly syndrome

Andrologia ◽  
2021 ◽  
Author(s):  
Masoud Habibi ◽  
Niloofar Sadeghi ◽  
Marziyeh Tavalaee ◽  
Abdolhossein Shahverdi ◽  
Zahra Ghorban ◽  
...  
Keyword(s):  
Sperm Chromatin ◽  
Chromatin Integrity

scholarly journals Single-center thorough evaluation and targeted treatment of globozoospermic men

2021 ◽  
Author(s):  
Stephanie Cheung ◽  
Alessandra Parrella ◽  
Danielle Tavares ◽  
Derek Keating ◽  
Philip Xie ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Gene Mutations ◽  
Calcium Ionophore ◽  
Immunofluorescence Assay ◽  
Sperm Chromatin ◽  
Reproductive Outcome ◽  
Saint Louis ◽  
Sperm Aneuploidy ◽  
Zygote Development ◽  
Chromatin Integrity

Abstract Purpose To characterize, by specific biomarkers and nucleic acid sequencing, the structural and genomic sperm characteristics of partial (PG) and complete globozoospermic (CG) men in order to identify the best reproductive treatment. Methods We assessed spermatozoa from 14 consenting men ultrastructurally, as well as for histone content, sperm chromatin integrity, and sperm aneuploidy. Additional genomic, transcriptomic, and proteomic evaluations were carried out to further characterize the CG cohort. The presence of oocyte-activating sperm cytosolic factor (OASCF) was measured by a phospholipase C zeta (PLCζ) immunofluorescence assay. Couples were treated in subsequent cycles either by conventional ICSI or by ICSI with assisted gamete treatment (AGT) using calcium ionophore (Ionomycin, 19657, Sigma-Aldrich, Saint Louis, MO, USA). Results Ultrastructural assessment confirmed complete acrosome deficiency in all spermatozoa from CG men. Histone content, sperm chromatin integrity, and sperm aneuploidy did not differ significantly between the PG (n = 4) and CG (n = 10) cohorts. PLCζ assessment indicated a positive presence of OASCF in 4 PG couples, who underwent subsequent ICSI cycles that yielded a 36.1% (43/119) fertilization with a 50% (2/4) clinical pregnancy and delivery rate. PLCζ assessment failed to detect OASCF for 8 CG patients who underwent 9 subsequent ICSI cycles with AGT, yielding a remarkable improvement of fertilization (39/97; 40.2%) (P = 0.00001). Embryo implantation (6/21; 28.6%) and clinical pregnancies (5/7; 71.4%) were also enhanced, resulting in 4 deliveries. Gene mutations (DPY19L2, SPATA16, PICK1) were identified in spermatozoa from CG patients. Additionally, CG patients unable to sustain a term pregnancy had gene mutations involved in zygote development (NLRP5) and postnatal development (BSX). CG patients who successfully sustained a pregnancy had a mutation (PIWIL1) related to sperm phenotype. PLCZ1 was both mutated and underexpressed in these CG patients, regardless of reproductive outcome. Conclusions Sperm bioassays and genomic studies can be used to characterize this gamete’s capacity to support embryonic development and to tailor treatments maximizing reproductive outcome.

Sperm Chromatin Integrity Tests and Indications

2020 ◽  
pp. 99-121
Author(s):  
Rakesh Sharma ◽  
Marlon P. Martinez ◽  
Ashok Agarwal
Keyword(s):  
Sperm Chromatin ◽  
Chromatin Integrity ◽  
Integrity Tests

scholarly journals Toxicants and human sperm chromatin integrity

2009 ◽  
Vol 16 (1) ◽  
pp. 14-22 ◽  
Author(s):  
G. Delbes ◽  
B. F. Hales ◽  
B. Robaire
Keyword(s):  
Human Sperm ◽  
Sperm Chromatin ◽  
Chromatin Integrity

scholarly journals Comparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentation

Andrologia ◽  
2020 ◽  
Author(s):  
Sulagna Dutta ◽  
Ralf Henkel ◽  
Ashok Agarwal
Keyword(s):  
Comparative Analysis ◽  
Dna Fragmentation ◽  
Sperm Chromatin ◽  
Chromatin Integrity

scholarly journals Beneficial effect of antioxidant therapy on sperm DNA integrity is not associated with a similar effect on sperm chromatin integrity

2019 ◽  
Vol 4 ◽  
pp. 31-31 ◽  
Author(s):  
Cécile Le Saint ◽  
Isaac-Jacques Kadoch ◽  
François Bissonnette ◽  
Julie Choi ◽  
Jonathan Zini ◽  
...  
Keyword(s):  
Beneficial Effect ◽  
Antioxidant Therapy ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Chromatin Integrity

Improvement of Rat Sperm Chromatin Integrity and Spermatogenesis with Omega 3 following Bleomycin, Etoposide and Cisplatin Treatment

2020 ◽  
pp. 1-9
Author(s):  
Shahnaz Razavi ◽  
Fatemeh Hashemi ◽  
Farnaz Khadivi ◽  
Abbas Bakhtiari ◽  
Amir Mokhtarian ◽  
...  
Keyword(s):  
Sperm Chromatin ◽  
Omega 3 ◽  
Chromatin Integrity

Sperm chromatin integrity of bucks transgenic for the WAP bGH gene

2000 ◽  
Vol 64 (1-2) ◽  
pp. 113-120 ◽  
Author(s):  
Piotr Gogol ◽  
Michał Bochenek ◽  
Zdzisław Smorąg
Keyword(s):  
Sperm Chromatin ◽  
Chromatin Integrity

scholarly journals Effects of the Chemotherapy Cocktail Used to Treat Testicular Cancer on Sperm Chromatin Integrity

2006 ◽  
Vol 28 (2) ◽  
pp. 241-249 ◽  
Author(s):  
G. Delbes ◽  
B. F. Hales ◽  
B. Robaire
Keyword(s):  
Testicular Cancer ◽  
Sperm Chromatin ◽  
Chromatin Integrity

scholarly journals Sperm chromatin integrity may predict future fertility for unexplained recurrent spontaneous abortion patients

2012 ◽  
Vol 35 (5) ◽  
pp. 752-757 ◽  
Author(s):  
Lihong Zhang ◽  
Leiguang Wang ◽  
Xingqi Zhang ◽  
Guanzhao Xu ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Recurrent Spontaneous Abortion ◽  
Sperm Chromatin ◽  
Future Fertility ◽  
Unexplained Recurrent Spontaneous Abortion ◽  
Chromatin Integrity
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