Establishment of in vitro model of erectile dysfunction for the study of high-glucose-induced angiopathy and neuropathy

Diabetic nephropathy is characterized by the chronic loss of kidney function due to high glucose renal levels. HK-2 proximal tubular cells are good candidates to study this disease. The aim of this work was to study an in vitro model of high glucose-induced metabolic alterations in HK-2 cells to contribute to the pathogenesis of this diabetic complication. An untargeted metabolomics strategy based on CE-MS was developed to find metabolites affected under high glucose conditions. Intracellular and extracellular fluids from HK-2 cells treated with 25 mM glucose (high glucose group), with 5.5 mM glucose (normal glucose group), and with 5.5 mM glucose and 19.5 mM mannitol (osmotic control group) were analyzed. The main changes induced by high glucose were found in the extracellular medium where increased levels of four amino acids were detected. Three of them (alanine, proline, and glutamic acid) were exported from HK-2 cells to the extracellular medium. Other affected metabolites include Amadori products and cysteine, which are more likely cause and consequence, respectively, of the oxidative stress induced by high glucose in HK-2 cells. The developed CE-MS platform provides valuable insight into high glucose-induced metabolic alterations in proximal tubular cells and allows identifying discriminative molecules of diabetic nephropathy.

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New in vitro model to study high glucose-dependent endothelial dysfunctions

Biochimie ◽

10.1016/s0300-9084(03)00116-0 ◽

2003 ◽

Vol 85 (7) ◽

pp. 701-705 ◽

Cited By ~ 4

Author(s):

Debora Donnini ◽

Elisa Del Terra ◽

Francesco Saverio Ambesi-Impiombato ◽

Francesco Curcio

Keyword(s):

High Glucose ◽

In Vitro Model ◽

Vitro Model ◽

Endothelial Dysfunctions

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The protective effects of metformin in an in vitro model of aging 3T3 fibroblast under the high glucose conditions

Journal of Physiology and Biochemistry ◽

10.1007/s13105-018-0613-5 ◽

2018 ◽

Vol 74 (2) ◽

pp. 273-281 ◽

Cited By ~ 11

Author(s):

Tugba Soydas ◽

Elif Yaprak Sarac ◽

Suzan Cinar ◽

Sibel Dogan ◽

Seyhun Solakoglu ◽

...

Keyword(s):

High Glucose ◽

In Vitro Model ◽

Protective Effects ◽

Vitro Model

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Role of resuscitation promoting factors in reactivation of latent tuberculosis during high glucose conditions employing human granuloma in vitro model

Microbial Pathogenesis ◽

10.1016/j.micpath.2021.105068 ◽

2021 ◽

pp. 105068

Author(s):

Arpana Verma ◽

Bishan D. Radotra ◽

Sadhna Sharma

Keyword(s):

High Glucose ◽

In Vitro Model ◽

Latent Tuberculosis ◽

Resuscitation Promoting Factors ◽

Vitro Model

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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