scholarly journals Syngeneic red blood cell–induced extracellular vesicles suppress delayed‐type hypersensitivity to self‐antigens in mice

2019 ◽  
Vol 49 (11) ◽  
pp. 1487-1499 ◽  
Author(s):  
Katarzyna Nazimek ◽  
Eugenio Bustos‐Morán ◽  
Noelia Blas‐Rus ◽  
Bernadeta Nowak ◽  
Włodzimierz Ptak ◽  
...  
Keyword(s):  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Delayed Type Hypersensitivity ◽  
Self Antigens

scholarly journals Red blood cell-derived extracellular vesicles mediate intercellular communication in ischemic heart failure

2019 ◽  
Author(s):  
Avash Das ◽  
Nedyalka Valkov ◽  
Ane M. Salvador ◽  
Ivan Kur ◽  
Olivia Ziegler ◽  
...  
Keyword(s):  
Heart Failure ◽  
Mouse Model ◽  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Significant Proportion ◽  
Ischemic Heart ◽  
Target Cells ◽  
Ischemic Heart Failure ◽  
Cellular Markers

SummaryExtracellular vesicles (EV) mediate intercellular signaling by transferring their cargo to recipient cells. Red blood cell (RBC)-derived EVs constitute a significant proportion of circulating EVs and have been implicated in regulating immune responses. Here, we describe a transgenic mouse model for fluorescent-based mapping of RBC-EV target cells based on the functional transfer of EV-contained Cre-recombinase to target cells. In a murine model of ischemic heart failure, we detect an increase in RBC-EV-targeted cardiomyocytes in the hearts and microglial cells in the brains. Cells targeted by RBC-EVs present an enrichment of genes implicated in cell proliferation and metabolism pathways compared to non-recombined (non-targeted) cells. Cardiomyocytes targeted by RBC-EVs are more likely to demonstrate cellular markers of DNA synthesis and proliferation, suggesting functional significance of EV-mediated signaling. In conclusion, we leverage our mouse model for mapping of RBC-EV targets in murine ischemic heart failure to demonstrate quantitative and qualitative changes in RBC-EV recipients.

Clearance and phenotype of extracellular vesicles after red blood cell transfusion in a human endotoxemia model

2019 ◽  
Vol 58 (4) ◽  
pp. 508-511 ◽  
Author(s):  
Lisa van Manen ◽  
Anna L. Peters ◽  
P. Matthijs van der Sluijs ◽  
Rienk Nieuwland ◽  
Robin van Bruggen ◽  
...  
Keyword(s):  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Red Blood Cell Transfusion ◽  
Human Endotoxemia ◽  
Endotoxemia Model

Monocyte-mediated activation of endothelial cells occurs only after binding to extracellular vesicles from red blood cell products, a process mediated by β-integrin

Transfusion ◽  
2016 ◽  
Vol 56 (12) ◽  
pp. 3012-3020 ◽  
Author(s):  
Marleen Straat ◽  
Maike E. van Hezel ◽  
Anita Böing ◽  
Anita Tuip-De Boer ◽  
Nina Weber ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell

scholarly journals The Fluid Membrane determines Mechanics of Red Blood Cell Extracellular Vesicles and is Softened in Hereditary Spherocytosis

2017 ◽  
Author(s):  
Daan Vorselen ◽  
Susan M. van Dommelen ◽  
Raya Sorkin ◽  
Jürgen Schiller ◽  
Richard van Wijk ◽  
...  
Keyword(s):  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Hereditary Spherocytosis ◽  
Cell Communication ◽  
Lipid Vesicle ◽  
Bending Modulus ◽  
Rbc Membrane ◽  
Blood Disorder ◽  
Healthy Donors

AbstractExtracellular vesicles (EVs) are widely studied regarding their role in cell-to-cell communication and disease, as well as for applications as biomarker or drug delivery vehicle. EVs contain both membrane and intraluminal proteins, affecting their structural properties and thereby likely their functioning. Here, we use atomic force microscopy for the mechanical characterization of red blood cell (RBC) EVs from healthy individuals as well as from a patient with hereditary spherocytosis (HS) due to ankyrin deficiency. We show that the EVs are packed with proteins, yet their response to indentation is similar to that of a fluid lipid vesicle lacking proteins. The bending modulus of RBC EVs of healthy donors is ~15kbT, agreeing well with the bending modulus of the RBC membrane. Surprisingly, whereas RBCs become more rigid in HS, the excreted vesicles of a patient with this blood disorder have a significantly (~50%) lower bending modulus than donor EVs. These results shed new light on the mechanism and effects of EV budding and may underlie the reported increase in vesiculation and stiffening of RBCs in hereditary spherocytosis patients.

scholarly journals Efficient RNA drug delivery using red blood cell extracellular vesicles

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Waqas Muhammad Usman ◽  
Tin Chanh Pham ◽  
Yuk Yan Kwok ◽  
Luyen Tien Vu ◽  
Victor Ma ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell

scholarly journals Extracellular Vesicles from Red Blood Cell Products Induce a Strong Pro-Inflammatory Host Response, Dependent on Both Numbers and Storage Duration

2015 ◽  
Vol 43 (4) ◽  
pp. 302-305 ◽  
Author(s):  
Marleen Straat ◽  
Anita N. Böing ◽  
Anita Tuip-De Boer ◽  
Rienk Nieuwland ◽  
Nicole P. Juffermans
Keyword(s):  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Host Response ◽  
Storage Duration ◽  
And Storage

Red blood cell-derived extracellular vesicles communicate with megakaryocyte–erythrocyte progenitor cells in bone marrow

2018 ◽  
Vol 102 ◽  
pp. 154
Author(s):  
Shulin Lu ◽  
Avash Das ◽  
Anne Nicholson-Weller ◽  
Horea Rus ◽  
Saumya Das ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Progenitor Cells ◽  
Extracellular Vesicles ◽  
Red Blood Cell

scholarly journals Red blood cell derived extracellular vesicles during the process of autologous blood doping

2021 ◽  
Author(s):  
S. C. Voss ◽  
M. Yassin ◽  
J. C. Grivel ◽  
S. Al Hmissi ◽  
N. Allahverdi ◽  
...  
Keyword(s):  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Autologous Blood ◽  
Blood Doping

scholarly journals Biosensing of red blood cell-derived extracellular vesicles with the advanced bright-field light optical polarization microscopy

2017 ◽  
Vol 3 (3) ◽  
pp. 61-65
Author(s):  
Alexander E. Berezin ◽  
◽  
Richard E Mokhnach ◽  
Harry A Byalik ◽  
Keyword(s):  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Polarization Microscopy ◽  
Optical Polarization ◽  
Bright Field
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