The erythrocyte membrane properties of beta thalassaemia heterozygotes and their consequences for Plasmodium falciparum invasion

Malaria parasites such as Plasmodium falciparum have exerted formidable selective pressures on the human genome. Of the human genetic variants associated with malaria protection, beta thalassaemia (a haemoglobinopathy) was the earliest to be associated with malaria prevalence. However, the malaria protective properties of beta thalassaemic erythrocytes remain unclear. Here we studied the mechanics and surface protein expression of beta thalassaemia heterozygous erythrocytes, measured their susceptibility to P. falciparum invasion, and calculated the energy required for merozoites to invade them. We found invasion-relevant differences in beta thalassaemic cells versus matched controls, specifically: elevated membrane tension, reduced bending modulus, and higher levels of expression of the major invasion receptor basigin. However, these differences acted in opposition to each other with respect to their likely impact on invasion, and overall we did not observe beta thalassaemic cells to have lower P. falciparum invasion efficiency for any of the strains tested.

Modelling of Auxetic Woven Structures for Composite Reinforcement

The current research is focused on the design and development of auxetic woven structures. Finite element analysis based on computational modeling and prediction of axial strain as well as Poisson’s ratio was carried out. Further, an analytical model was used to calculate the same parameters by a foldable zig-zag geometry. In the analytical model, Poisson’s ratio is based on the crimp percentage, bending modulus, yarn spacing, and coefficient of friction. In this yarn, properties and fabric parameters were also considered. Experimental samples were evaluated for the actual performance of the defined auxetic material. Auxetic fabric was developed with foldable strips created in a zig-zag way in the vertical (warp) direction. It is based on the principle that when the fabric is stretched, the unfolding of the folds takes place, leading to an increase in transverse dimensions. Both the analytical and computational models gave close predictions to the experimental results. The fabric with foldable strips created in a zig-zag way in the vertical (warp) direction produced negative Poisson’s ratio (NPR), up to 8.7% of axial strain, and a maximum Poisson’s ratio of −0.41 produced at an axial strain of around 1%. The error percentage in the analytical model was 37.14% for the experimental results. The computational results also predict the Poisson’s ratio with an error percentage of 22.26%. Such predictions are useful for estimating the performance of auxetic woven structures in composite reinforcement. The auxetic structure exhibits remarkable stress-strain behavior in the longitudinal as well as transverse directions. This performance is useful for energy absorption in composite reinforcement.

Graphene coated cotton nonwoven for electroconductive and UV protection applications

The functional properties and applications of graphene coated textiles depend on the magnitude of graphene add-on which in turn is influenced by the type of substrate and the dipping conditions. In the present study, optimized GO (graphene oxide) dipping conditions are identified for the preparation of cost-effective and scalable rGO (reduced graphene oxide) coated cotton nonwoven for electroconductive and UV (ultraviolet) blocking applications. To understand the influence of GO dipping variables on rGO add-on and electrical resistivity of cotton, batch adsorption studies are carried out in loose fibre form to eliminate the structural influence of yarn or fabric. Batch adsorption studies suggest that GO concentration, pH of GO solution and sodium dithionite (reductant) concentration are the most influencing dipping variables and these dipping variables are optimized for cotton nonwoven fabric using Box–Behnken response surface design to achieve minimum surface resistivity. The rGO coated cotton nonwoven fabric shows excellent UV blocking properties (UV protection factor = 89.38) at the optimized GO dipping conditions. Physical properties of cotton nonwoven fabric such as GSM, thickness, stiffness, breaking strength and elongation are analysed at different dipping cycles. After the rGO coating, bending rigidity, bending modulus and breaking elongation of the cotton nonwoven fabric decrease, whereas the breaking strength increases. rGO coated cotton fabric exhibits excellent stability towards multiple washing and rubbing. The graphene coated cotton is characterised by FT-IR, XRD, Raman, TGA, FESEM and LEICA image analyser.

Surface Properties of Synaptosomes in the Presence of L-Glutamic and Kainic Acids: In Vitro Alteration of the ATPase and Acetylcholinesterase Activities

Morphologically and functionally identical to brain synapses, the nerve ending particles synaptosomes are biochemically derived membrane structures responsible for the transmission of neural information. Their surface and mechanical properties, measured in vitro, provide useful information about the functional activity of synapses in the brain in vivo. Glutamate and kainic acid are of particular interest because of their role in brain pathology (including causing seizure, migraine, ischemic stroke, aneurysmal subarachnoid hemorrhage, intracerebral hematoma, traumatic brain injury and stroke). The effects of the excitatory neurotransmitter L-glutamic acid and its agonist kainic acid are tested on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic membranes prepared from the cerebral cortex of rat brain tissue. The surface parameters of synaptosome preparations from the cerebral cortex in the presence of L-glutamic and kainic acids are studied by microelectrophoresis for the first time. The studied neurotransmitters promote a significant increase in the electrophoretic mobility and surface electrical charge of synaptosomes at 1–4 h after isolation. The measured decrease in the bending modulus of model bimolecular membranes composed of monounsaturated lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine provides evidence for softer membranes in the presence of L-glutamate. Kainic acid does not affect membrane mechanical stability even at ten-fold higher concentrations. Both the L-glutamic and kainic acids reduce acetylcholinesterase activity and deviation from the normal functions of neurotransmission in synapses is presumed. The presented results regarding the modulation of the enzyme activity of synaptic membranes and surface properties of synaptosomes are expected by biochemical and biophysical studies to contribute to the elucidation of the molecular mechanisms of neurotransmitters/agonists’ action on membranes.

Deformability of discs in turbulence

The aim of this study is to investigate experimentally the transition from a rigid regime to a deformed regime for flexible discs freely advected in turbulent flows. For a given disc, the amplitude of the deformation is expected to increase when its bending modulus decreases or when the turbulent kinetic energy increases. To quantify this qualitative argument, experiments are performed where the deformation of flexible discs is measured using three cameras. The amplitude of the deformation has been characterised by the eigenvalues of the moment of inertia tensor. Experimental results exhibit a transition from a rigid regime to a deformed regime that depends on the size, the density and the flexibility of the disc and the turbulent kinetic energy. The modelling of this transition is a generalisation and an extension of the previous models used to characterise the deformation of flexible fibres in turbulent flows.

Evaluation of Low-Grade Yellow-Poplar (Liriodendron tulipifera) as Raw Material for Cross-Laminated Timber Panel Production

Utilization of low-grade yellow-poplar (Liriodendron tulipifera) lumber would provide for alternative structural lumber sources and promote the growth of cross-laminated timber (CLT) manufacturing facilities within the Appalachian Region. A significant amount of low-grade yellow-poplar lumber (i.e., National Hardwood Lumber Association [NHLA] No. 2A and Below Grade) is utilized for wood pallets. In practice, this material is not graded for structural purposes. Additionally, research on yellow-poplar for structural use has focused on grading lumber from a small population of selected logs, not by regrading NHLA lumber from manufacturing facilities. Therefore, the research's objective was to investigate the structural grades of a typical population of NHLA graded No. 2 and lower lumber and evaluate their potential to meet structural grades necessary for CLT panels. NHLA graded lumber was regraded and assigned to visual structural grades following Northeastern Lumber Manufacturers Association rules and evaluated for flatwise bending modulus of elasticity (MOEb) by nondestructive proof loading. The results of the study indicated that 54.6 percent of the boards possessed a minimal structural visual grade required for CLT panels according to American National Standards Institutes/The Engineered Wood Association (ANSI/APA) PRG 320-2019 (2020). Splits were the most common limiting defect that downgraded boards to nonstructural grades. Also, 96.6 percent of the boards evaluated had a MOEb above the required minimal board value of 1.2 ×106 psi (8,274 MPa) listed in ANSI/APA PRG 320-2019 (2020). The results of the study indicated that a majority of NHLA low-grade yellow-poplar, when regraded for structural purposes, meets or exceeds minimum lumber grade values necessary for use in CLT panel production.

The HDPE composites reinforced with waste hybrid PET/cotton fibers modified with the synthesized modifier

Large amounts of textile waste are generated every year and disposed of through landfill or incineration, leading to numerous environmental and social issues. In this study, waste hybrid polyethylene terephthalate (PET)/cotton fibers were used directly to reinforce high density polyethylene (HDPE) to prepare composites. In order to give full play to the fiber’s reinforcing characteristics, the PET/cotton fibers were further modified with the modifier using a novel synthesized tetraethyl orthosilicate/3-aminopropyl triethoxysilane (KH550)/polyethylene (PE)-g-MAH (MPE) hybrid (TMPE). Fourier transform infrared and scanning electron microscopy (SEM) confirmed that the TMPE was successfully coated on the surface of fibers. Furthermore, compared with the original and the MPE-modified fibers, the thermal stability of TMPE-modified fibers was significantly increased. SEM and mechanical test indicated that the compatibility of the modified fibers with HDPE had been significantly improved, which led to the improvement of mechanical properties. Compared with the original and MPE-modified fibers-reinforced HDPE composites, the bending strength, bending modulus, and impact strength of TMPE-modified fiber-reinforced HDPE composites were improved obviously by 31.7%, 25.7%, and 89.1%, respectively.

Realization of active metamaterials with odd micropolar elasticity

Materials made from active, living, or robotic components can display emergent properties arising from local sensing and computation. Here, we realize a freestanding active metabeam with piezoelectric elements and electronic feed-forward control that gives rise to an odd micropolar elasticity absent in energy-conserving media. The non-reciprocal odd modulus enables bending and shearing cycles that convert electrical energy into mechanical work, and vice versa. The sign of this elastic modulus is linked to a non-Hermitian topological index that determines the localization of vibrational modes to sample boundaries. At finite frequency, we can also tune the phase angle of the active modulus to produce a direction-dependent bending modulus and control non-Hermitian vibrational properties. Our continuum approach, built on symmetries and conservation laws, could be exploited to design others systems such as synthetic biofilaments and membranes with feed-forward control loops.

A Comparison among Lignin Modification Methods on the Properties of Lignin–Phenol–Formaldehyde Resin as Wood Adhesive

The research aim of this work is to determine the influence of lignin modification methods on lignin–phenol–formaldehyde (LPF) adhesive properties. Thus, glyoxal (G), phenol (P), ionic liquid (IL), and maleic anhydride (MA) were used to modify lignin. The modified lignins were used for phenol substitution (50 wt%) in phenol–formaldehyde adhesives. The prepared resins were then used for the preparation of wood particleboard. These LPF resins were characterized physicochemically, namely by using standard methods to determine gel time, solids content, density, and viscosity, thus the physicochemical properties of the LPF resins synthesized. The panels dimensional stability, formaldehyde emission, bending modulus, bending strength, and internal bond (IB) strength were also measured. MA-modified lignin showed by differential scanning calorimetry (DSC) the lowest temperature of curing than the resins with non-modified lignin and modified with IL, phenolared lignin, and glyoxal. LPF resins with lignin treated with maleic anhydride presented a shorter gel time, higher viscosity, and solids content than the resins with other lignin modifications. Equally, the particleboard panels prepared with LPF resins with maleic anhydride or with ionic liquid had the lowest formaldehyde emission and the highest mechanical strength among all the synthesized resins. The dimensional stability of all panels bonded with modified lignin LPF resins presented no difference of any significance.