Component resolved diagnosis using guinea‐pig allergens elucidates allergen sensitization profiles in allergy to furry animals

Aim: to study the sensitization profile in patients with sensitivity to Bet v1 allergen who have clinically significant respiratory symptoms of pollinosis during the birch flowering season, oral allergy syndrome (OAS), and angioedema to nuts and stone fruits. Patients and Methods: a prospective open-label study included 40 children (average age 7.52±0.64), 26 (65%) boys and 14 (35%) girls. All children had sensitization to the birch major allergen (Bet v1), symptoms of respiratory allergy during the birch flowering season, manifestations of OAS with angioedema in combination with hives or without it after eating fruits of Rosaceae family/nuts/peanuts. Children underwent general clinical and allergy researches, determination of total IgE and eosinophil cationic protein. By the method of component-resolved diagnosis of allergy (ISAC-112, ImmunoCAP), a comprehensive allergology examination for 112 allergen molecules was performed for each child. Results: the children of the study group were found to have increased values of total IgE (256.66±40.45 IU/mL) and eosinophil cationic protein (67.22±8.67 ng/mL). It was found that 57.5% of children were sensitized to tree nut storage proteins (rJug r1, rJug r2, NCoR a9, rAna o2, rBer e1), and some patients (27.5%) had increased specific IgE to non-specific lipid-transfer proteins (npLTPs) (rPru p3, NCoR a8, rAra h9, rJug r3). Antibodies to peanut storage proteins (rAra h1, rAra h2, rAra h3, rAra h6) were in 22.5% of children in the study group. Conclusion: the component-resolved diagnosis of allergy allows to determine the sensitization profile of patients. Sensitization detection to storage proteins and/or nsLTPs of nuts, peanuts and fruits is a predictor of possible severe allergic reactions. The exclusion of these products, both raw and heat-treated, reduces the number of severe acute allergic reactions in patients and prevents possible fatal outcomes. KEYWORDS: oral allergy syndrome, angioedema, children, birch, sensitization to Bet v1 allergen, storage proteins, nsLTPs. FOR CITATION: Lepeshkova T.S., Beltyukov E.K., Tsarkova S.A., Naumova V.V. Oral allergy syndrome and angioedema in patients with birch allergen sensitization: are there any high risks? Russian Medical Inquiry. 2021;5(1):21–24. DOI: 10.32364/2587-6821-2021-5-1-21-24.

Download Full-text

Artemisia pollen allergy in China: Component-resolved diagnosis reveals allergic asthma patients have significant multiple allergen sensitization

Allergy ◽

10.1111/all.13597 ◽

2018 ◽

Vol 74 (2) ◽

pp. 284-293 ◽

Cited By ~ 11

Author(s):

Zhongshan Gao ◽

Wan-Yi Fu ◽

Yuemei Sun ◽

Biyuan Gao ◽

Hui-Ying Wang ◽

...

Keyword(s):

Allergic Asthma ◽

Pollen Allergy ◽

Allergen Sensitization ◽

Component Resolved Diagnosis ◽

Asthma Patients ◽

Artemisia Pollen

Download Full-text

Decision letter for "The Effect of Optic Nerve Section on Form Deprivation Myopia in the Guinea Pig"

10.1002/cne.24961/v1/decision1 ◽

2020 ◽

Keyword(s):

Optic Nerve ◽

Guinea Pig ◽

Nerve Section

Download Full-text

Effect of Contraceptive Steroids on the Endometrium of Guinea Pig: SEM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080766 ◽

1977 ◽

Vol 35 ◽

pp. 668-669

Author(s):

Mai M. Said ◽

Ramesh K. Nayak ◽

Randall E. McCoy

Keyword(s):

Guinea Pig ◽

Reproductive Tract ◽

Three Dimensional ◽

Female Reproductive Tract ◽

Human Female ◽

Surface Ultrastructure ◽

Transmission Electron ◽

Sem Study ◽

Uterine Mucosa ◽

Group 1

Burgos and Wislocki described changes in the mucosa of the guinea pig uterus, cervix and vagina during the estrous cycle investigated by transmission electron microscopy. More recently, Moghissi and Reame reported the effects of progestational agents on the human female reproductive tract. They found drooping and shortening of cilia in norgestrel and norethindrone- treated endometria. To the best of our knowledge, no studies concerning the effects of mestranol and norethindrone given concurrently on the three-dimensional surface features on the uterine mucosa of the guinea pig have been reported. The purpose of this study was to determine the effect of mestranol and norethindrone on surface ultrastructure of guinea pig uterus by SEM.Seventy eight animals were used in this study. They were allocated into two groups. Group 1 (20 animals) was injected intramuscularly 0.1 ml vegetable oil and served as controls.

Download Full-text

Ultrastructure and Drug Metabolism in the Developing Guinea Pig

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073131 ◽

1973 ◽

Vol 31 ◽

pp. 538-539

Author(s):

W. Kuenzig ◽

M. Boublik ◽

J.J. Kamm ◽

J.J. Burns

Keyword(s):

Guinea Pig ◽

Metabolic Activity ◽

Animal Species ◽

Adult Animal ◽

Smooth Endoplasmic Reticulum ◽

Fetal Life ◽

Mixed Function Oxidase ◽

Cytochrome P 450 ◽

Microsomal Mixed Function Oxidase ◽

Mixed Function Oxidase Activity

Unlike a variety of other animal species, such as the rabbit, mouse or rat, the guinea pig has a relatively long gestation period and is a more fully developed animal at birth. Kuenzig et al. reported that drug metabolic activity which increases very slowly during fetal life, increases rapidly after birth. Hepatocytes of a 3-day old neonate metabolize drugs and reduce cytochrome P-450 at a rate comparable to that observed in the adult animal. Moreover the administration of drugs like phenobarbital to pregnant guinea pigs increases the microsomal mixed function oxidase activity already in the fetus.Drug metabolic activity is, generally, localized within the smooth endoplasmic reticulum (SER) of the hepatocyte.

Download Full-text

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077207 ◽

1983 ◽

Vol 41 ◽

pp. 726-727

Author(s):

Corazon D. Bucana

Keyword(s):

Bone Marrow ◽

Guinea Pig ◽

Electron Density ◽

Peritoneal Cavity ◽

Ultrastructural Study ◽

Guinea Pigs ◽

Random Distribution ◽

Glycogen Content ◽

Polymorphonuclear Neutrophils ◽

Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

Download Full-text

Ultrastructural Studies on Genital Infection with the Chlamydial Agent of Guinea Pig Inclusion Conjunctivitis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079267 ◽

1977 ◽

Vol 35 ◽

pp. 352-353

Author(s):

B. L. Soloff ◽

A. L. Barron ◽

H. J. White ◽

R. G. Rank

Keyword(s):

Guinea Pig ◽

Sexual Contact ◽

Chlamydia Psittaci ◽

Genital Infection ◽

Major Site ◽

Ultrastructural Studies ◽

Tissue Specimens ◽

Guinea Pig Model ◽

Adequate Tissue ◽

Genital Tissue

Chlamydial organisms (specifically C. trachomatis) have been implicated as a frequent cause of genital infection in the human (1). Study of the histo- pathological aspects of such infections has been impeded because of difficulties in obtaining adequate tissue specimens and the lack of a suitable experimental host. In 1964, Murray (2) isolated the causative agent of guinea pig inclusion conjunctivitis which possesses similarities to human inclusion conjunctivitis. This guinea pig organism was found to be a member of the Chlamydia psittaci subgroup and was designated as the Gp-ic agent. Male guinea pigs have been successfully infected with Gp-ic by intraurethral inoculation. Transmission of the infection to the female by sexual contact has been demonstrated (3). We are not aware of any ultrastructural studies to date concerning the development of this agent in genital tissue.Studies in our laboratory have established that, in our guinea pig model, the cervix is the major site of injection.

Download Full-text