Two cases of Cytochrome P450 oxidoreductase deficiency with severe scoliosis and surgery requirement

2021 ◽  
Author(s):  
Takanori Onuki ◽  
Yoshiaki Ohtsu ◽  
Shota Hiroshima ◽  
Kentaro Sawano ◽  
Keisuke Nagasaki
Keyword(s):  
Cytochrome P450 ◽  
Severe Scoliosis ◽  
P450 Oxidoreductase ◽  
Cytochrome P450 Oxidoreductase

scholarly journals An electron transfer competent structural ensemble of membrane-bound cytochrome P450 1A1 and cytochrome P450 oxidoreductase

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Goutam Mukherjee ◽  
Prajwal P. Nandekar ◽  
Rebecca C. Wade
Keyword(s):  
Electron Transfer ◽  
Cytochrome P450 ◽  
Drug Targets ◽  
Catalytic Domain ◽  
Cytochrome P450 1A1 ◽  
P450 Oxidoreductase ◽  
Distal Side ◽  
Cytochrome P450 Oxidoreductase ◽  
Membrane Bound ◽  
Transient Interactions

AbstractCytochrome P450 (CYP) heme monooxygenases require two electrons for their catalytic cycle. For mammalian microsomal CYPs, key enzymes for xenobiotic metabolism and steroidogenesis and important drug targets and biocatalysts, the electrons are transferred by NADPH-cytochrome P450 oxidoreductase (CPR). No structure of a mammalian CYP–CPR complex has been solved experimentally, hindering understanding of the determinants of electron transfer (ET), which is often rate-limiting for CYP reactions. Here, we investigated the interactions between membrane-bound CYP 1A1, an antitumor drug target, and CPR by a multiresolution computational approach. We find that upon binding to CPR, the CYP 1A1 catalytic domain becomes less embedded in the membrane and reorients, indicating that CPR may affect ligand passage to the CYP active site. Despite the constraints imposed by membrane binding, we identify several arrangements of CPR around CYP 1A1 that are compatible with ET. In the complexes, the interactions of the CPR FMN domain with the proximal side of CYP 1A1 are supplemented by more transient interactions of the CPR NADP domain with the distal side of CYP 1A1. Computed ET rates and pathways agree well with available experimental data and suggest why the CYP–CPR ET rates are low compared to those of soluble bacterial CYPs.

Insight into the structural and functional analysis of the impact of missense mutation on cytochrome P450 oxidoreductase

2020 ◽  
Vol 100 ◽  
pp. 107708
Author(s):  
Rajalakshmi Kumar ◽  
Manikandan Jayaraman ◽  
Krishna Ramadas ◽  
Adithan Chandrasekaran
Keyword(s):  
Functional Analysis ◽  
Cytochrome P450 ◽  
Missense Mutation ◽  
P450 Oxidoreductase ◽  
Cytochrome P450 Oxidoreductase ◽  
The Impact ◽  
Insight Into

scholarly journals Human Cytochrome P450 Oxidoreductase Deficiency Caused by the Y181D Mutation: Molecular Consequences and Rescue of Defect

2009 ◽  
Vol 38 (2) ◽  
pp. 332-340 ◽  
Author(s):  
Christopher C. Marohnic ◽  
Satya P. Panda ◽  
Karen McCammon ◽  
José Rueff ◽  
Bettie Sue Siler Masters ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
P450 Oxidoreductase ◽  
Human Cytochrome ◽  
Cytochrome P450 Oxidoreductase

Influence of genetic polymorphisms in cytochrome P450 oxidoreductase on the variability in stable warfarin maintenance dose in Han Chinese

2016 ◽  
Vol 72 (11) ◽  
pp. 1327-1334 ◽  
Author(s):  
Wu-Tao Zeng ◽  
Qing Xu ◽  
Cheng-Hsun Li ◽  
Wei-Yan Chen ◽  
Xiu-Ting Sun ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Genetic Polymorphisms ◽  
Maintenance Dose ◽  
Han Chinese ◽  
P450 Oxidoreductase ◽  
Cytochrome P450 Oxidoreductase ◽  
Warfarin Maintenance Dose

Drosophila melanogaster NADPH–cytochrome P450 oxidoreductase: pronounced expression in antennae may be related toodorantclearance

Gene ◽  
1997 ◽  
Vol 189 (2) ◽  
pp. 213-219 ◽  
Author(s):  
Bernhard T Hovemann ◽  
Frank Sehlmeyer ◽  
Jochen Malz
Keyword(s):  
Drosophila Melanogaster ◽  
Cytochrome P450 ◽  
P450 Oxidoreductase ◽  
Nadph Cytochrome ◽  
Cytochrome P450 Oxidoreductase
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