Going further on an intricate and challenging group of nudibranchs: description of five novel species and a more complete molecular phylogeny of the subfamily Nembrothinae (Polyceridae)

During a survey of keratinolytic fungi in China, three Cunninghamella strains were isolated. Phylogenetic analyses of ITS and ITS+LSU+EF-1α sequence data showed that these strains constitute a new species related to C. blakesleeana, C. bigelovii, C. multiverticillata and C. phaeospora. The new species differs from C. multiverticillata and C. phaeospora in the shape and size of its teminal and lateral vesicles and can be distinguished from C. blakesleeana and C. bigelovii by the absent of zygosporangia, and the shape and size of it sporangioles. The results of phylogenetic and morphological analyses indicate that the three strains are a new species of Cunninghamella. Descriptions and illustrations of this novel species are provided in this paper.

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Three new species of Russulaceae from India based on morphology and molecular phylogeny

Phytotaxa ◽

10.11646/phytotaxa.246.1.5 ◽

2016 ◽

Vol 246 (1) ◽

pp. 61 ◽

Cited By ~ 2

Author(s):

K. P. Deepna Latha ◽

K. N. Anil Raj ◽

V. Adnaan Farook ◽

Shahina A. Sharafudheen ◽

Neeraja K. Parambil ◽

...

Keyword(s):

New Species ◽

Maximum Likelihood ◽

Molecular Phylogeny ◽

Internal Transcribed Spacer ◽

Novel Species ◽

Large Subunit ◽

Line Drawings ◽

Three New Species ◽

Kerala State ◽

Blastn Search

Three new species of Russulaceae, Lactifluus umbonatus and Lactifluus indicus belonging to Lf. subg. Gerardii and Lactarius keralensis belonging to L. subg. Piperites, are described from Kerala State, India. Comprehensive descriptions, photographs, line drawings and comparisons with phenetically similar and phylogenetically related species are given. Nuclear ribosomal internal transcribed spacer regions (ITS) of the three species were sequenced and analyzed. In addition, nuclear ribosomal large subunit (nLSU) gene of Lactifluus indicus was sequenced and the sequence was used in BLASTn search to find similar sequences. Maximum likelihood (ML) analysis based on the ITS sequences confirmed both the novelty of these species and their placement within their respective genera. The discovery of Lactifluus umbonatus and Lf. indicus represent the first report of novel species belonging to Lf. subg. Gerardii from India. Lactifluus indicus represents the second record of a pleurotoid Lactifluus species from India.

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Molecular systematics of the Acmena alliance (Myrtaceae): phylogenetic analyses and evolutionary implications with reference to Australian taxa

Australian Systematic Botany ◽

10.1071/sb03009 ◽

2004 ◽

Vol 17 (1) ◽

pp. 63 ◽

Cited By ~ 15

Author(s):

M. G. Harrington ◽

P. A. Gadek

Keyword(s):

Bayesian Inference ◽

Molecular Phylogeny ◽

Molecular Systematics ◽

Novel Species ◽

Phylogenetic Analyses ◽

Morphological Features ◽

Parsimony Analysis ◽

Species Groups ◽

Difficult Sequences

The existence within Australia of several Acmena alliance taxa with morphological features inconsistent with current generic boundaries has meant that phylogenetic assessment of relationships has proved difficult. Sequences of nrDNA from the ITS and ETS regions were obtained for 66 Australian taxa, including representatives from both subfamilies and five genera. The ingroup taxa included 54 Australian endemics and 13 species with distributions that also range outside continental Australia. Parsimony analysis and Bayesian inference of posterior possibilities from the combined molecular datasets do not correlate with the species associations implied by the current taxonomic circumscription of genera: Acmena, Acmenosperma, Anetholea, Syzygium and Waterhousea. The molecular phylogeny recognises five supported lineages within the Acmena alliance, and several novel species associations. The recognition of five species groups indicates a need for a reappraisal of all currently recognised morphological groups within the alliance.

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Corrigendum: He XL, Horak E, Wang D, Li TH, Peng WH, Gan BC (2019) Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l. MycoKeys 61: 1–26. https://doi.org/10.3897/mycokeys.61.46446

MycoKeys ◽

10.3897/mycokeys.63.49739 ◽

2020 ◽

Vol 63 ◽

pp. 163-172

Author(s):

Xiao-Lan He ◽

Egon Horak ◽

Di Wang ◽

Tai-Hui Li ◽

Wei-Hong Peng ◽

...

Keyword(s):

New Species ◽

Species Diversity ◽

Molecular Phylogeny ◽

Novel Species ◽

Molecular Data ◽

Monophyletic Group ◽

Molecular Evidence ◽

Morphological And Molecular Data ◽

High Level ◽

Ecological Range

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, &ldquo;Alboleptonia&rdquo;, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

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Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l.

MycoKeys ◽

10.3897/mycokeys.61.46446 ◽

2019 ◽

Vol 61 ◽

pp. 1-26

Author(s):

Xiao-Lan He ◽

Egon Horak ◽

Di Wang ◽

Tai-Hui Li ◽

Wei-Hong Peng ◽

...

Keyword(s):

New Species ◽

Species Diversity ◽

Molecular Phylogeny ◽

Novel Species ◽

Molecular Data ◽

Monophyletic Group ◽

Molecular Evidence ◽

Morphological And Molecular Data ◽

High Level ◽

Ecological Range

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

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Molecular phylogeny of Aplosporella abexaminans: a novel species revealing the second report of sexual-asexual connection in Aplosporellaceae (Botryosphaeriales) from India

Phytotaxa ◽

10.11646/phytotaxa.525.3.3 ◽

2021 ◽

Vol 525 (3) ◽

pp. 205-222

Author(s):

SANJEET KUMAR VERMA ◽

SANJAY YADAV ◽

RAGHVENDRA SINGH

Keyword(s):

Molecular Phylogeny ◽

Sequence Data ◽

Novel Species ◽

Morphological Characteristics ◽

Molecular Evidence ◽

Murraya Koenigii ◽

Asexual Morph ◽

Sexual Morph ◽

Asexual State

Aplosporella abexaminans, a new sexual morph fungus belongs to family Aplosporellaceae, was discovered on the bark of stem of Murraya koenigii (Rutaceae) and identified by morphological characteristics and analysis of combined ITS and LSU sequence data. This is the second report of a sexual morph with molecular evidence for this genus and the second record of conidiogenesis and chlamydospores associated with the asexual state of this family. It is characterized by its larger ascostromata, locules without ostioles, thinner locule peridium, two to multi-layered, larger asci, surrounded by an additional hyaline sac like structure, larger and hyaline to light olivaceous ascospores, asexual state without conidiomata formation, conidiogenous cells light brown to brown, conidia brown without granular content, smooth and presence of chlamydospores. The sexual morph of Aplosporella abexaminans resembles Bagnisiella and the asexual morph resembles Aplosporella, thus proving the sexual-asexual connection for the second time for this family.

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Taxonomy, ontogeny and molecular phylogeny of Anteholosticha marimonilata spec. nov. (Ciliophora, Hypotrichida) from the Yellow Sea, China

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.024638-0 ◽

2011 ◽

Vol 61 (8) ◽

pp. 2000-2014 ◽

Cited By ~ 15

Author(s):

Yuan Xu ◽

Jie Huang ◽

Xiaozhong Hu ◽

Khaled A. S. Al-Rasheid ◽

Weibo Song ◽

...

Keyword(s):

Molecular Phylogeny ◽

Yellow Sea ◽

Gene Sequence ◽

Novel Species ◽

Phylogenetic Analyses ◽

The Yellow Sea ◽

Rrna Gene ◽

Sequence Information ◽

Rrna Gene Sequence ◽

Cortical Granules

The morphology, morphogenesis and molecular phylogeny of the marine ciliated protozoan Anteholosticha marimonilata spec. nov., isolated from mollusc-farming waters of the Yellow Sea, Qingdao, PR China, were investigated using microscopic observations of live and protargol-impregnated specimens and by small subunit rRNA gene sequence analysis. The novel species could be distinguished by the following features: an elongated elliptical body, in vivo size 80–160 µm × 30–50 µm; an adoral zone consisting of about 36 membranelles; three frontal, one parabuccal, one buccal, two frontoterminal and usually two pretransverse ventral cirri; 10–13 transverse cirri; a midventral complex composed of 12–17 pairs of cirri only, terminating in posterior 1/5; four or five dorsal kineties; two types of colourless cortical granules; four to nine moniliform macronuclear nodules and one to three micronuclei, and a contractile vacuole positioned at mid-body. Hitherto, the ontogenesis of the genus Anteholosticha has been regarded as rather diverse, which was confirmed by the morphogenetic processes of this novel species. The most noteworthy feature of A. marimonilata was that the proter retained almost the entire parental adoral zone except for a few proximal membranelles that were renewed in situ. The SSU rRNA gene sequence information clearly discriminated this isolate from its congeners. Molecular phylogenetic analyses demonstrated, with high statistical support, that A. marimonilata branched as a sister lineage to the Nothoholosticha–Pseudokeronopsis clade and hence belongs to the core part of the order Urostylida.

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Morphology, morphogenesis and molecular phylogeny of a novel soil ciliate, Pseudouroleptus plestiensis n. sp. (Ciliophora, Oxytrichidae), from the uplands of Colfiorito, Italy

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.062885-0 ◽

2014 ◽

Vol 64 (Pt_8) ◽

pp. 2625-2635 ◽

Cited By ~ 6

Author(s):

Daizy Bharti ◽

Santosh Kumar ◽

Antonietta La Terza

Keyword(s):

Molecular Phylogeny ◽

Novel Species ◽

Phylogenetic Analyses ◽

Rrna Gene ◽

Ssu Rrna ◽

The Novel ◽

Gene Sequences ◽

Ssu Rrna Gene ◽

Umbria Region ◽

The Right

The terrestrial oxytrichid ciliate Pseudouroleptus plestiensis n. sp., isolated from soil samples collected from the uplands of Colfiorito (Umbria region, Italy), was investigated using live observation and protargol impregnation. The morphology, morphogenesis and molecular phylogeny inferred from small-subunit (SSU) rRNA gene sequences were studied. The novel species is mainly characterized by the following: a cell size of about 145×35 µm in vivo; two ellipsoidal macronuclear nodules and two to four micronuclei; adoral zone about 26 % of body length with a mean of 30 membranelles; about 40 cirri in the right marginal row and 38 in the left marginal row; left fronto-ventral row consisting of about 27–40 cirri, right fronto-ventral row of about three to seven cirri forming a short row to the right of the rear portion of the left fronto-ventral row; one parabuccal cirrus ( = III/2), one buccal and one post-peristomial cirrus; and four dorsal kineties with caudal cirri at the end of kineties 1 and 2. The morphogenesis of the novel species is similar to that of Pseudouroleptus caudatus. Phylogenetic analyses based on SSU rRNA gene sequences consistently placed the novel species within the family Oxytrichidae Ehrenberg, 1838, clustering with P. caudatus and the genus Strongylidium. The results from the present study contribute to the expanding knowledge of the diversity of ciliates in Italian soil.

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Pythium huanghuaiense sp. nov. isolated from soybean: morphology, molecular phylogeny and pathogenicity

Biodiversity Data Journal ◽

10.3897/bdj.9.e65227 ◽

2021 ◽

Vol 9 ◽

Author(s):

Jia-Jia Chen ◽

Hui Feng ◽

Jian Yu ◽

Wenwu Ye ◽

Xiaobo Zheng

Keyword(s):

Glycine Max ◽

Molecular Phylogeny ◽

Novel Species ◽

Protein A ◽

Morphological Characters ◽

Edible Oil ◽

Molecular Evidence ◽

Pathogenicity Test ◽

Soybean Seedlings ◽

Thin Walled

Soybean (Glycine max) is a major source of edible oil and protein. A novel species of the genus Pythium, Pythium huanghuaiense, isolated from soybean seedlings in China, is described and illustrated on the basis of morphological characters and molecular evidence. Pythium huanghuaiense sp. nov. is closely related to species of the genus Pythium in clade F, as evidenced by the presence of hyphal swellings and its relatively rapid morphological growth. However, it differs by having relatively small sporangia and plerotic or nearly plerotic and thin-walled oospores. A pathogenicity test confirmed the newly-identified species as a pathogen of soybean.

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