Isolation and Characterization of a Biosurfactant Producing Strain Planococcus sp. XW-1 from the Cold Marine Environment

One cold-adapted strain, named Planococcus sp. XW-1, was isolated from the Yellow Sea. The strain can produce biosurfactant with petroleum as sole source of carbon at low temperature (4 °C). The biosurfactant was identified as glycolipid-type biosurfactant species by thin-layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). It reduced the surface tension of water to 26.8 mN/m with a critical micelle concentration measurement of 60 mg/L. The produced biosurfactant possesses high surface activity at wide ranges of temperature (−18–105 °C), pH values (2–12), and salt concentrations (1–18%). The biosurfactant exhibited higher surface activity and higher growth rate of cells with hexadecane and diesel as carbon source. The strain Planococcus sp. XW-1 was also effective in degrading crude oil, after 21 days of growth at 4 °C in medium with 1% crude oil and 1% (v/v) bacteria broth, 54% of crude oil was degraded. The results suggest that Planococcus sp. XW-1 is a promising candidate for use in the bioremediation of petroleum-contaminated seawater in the Yellow Sea during winter. This study reported for the first time that Planococcus isolated from the Yellow Sea can produce biosurfactant using petroleum as the sole carbon source at low temperature (4 °C), showing its ecological role in the remediation of marine petroleum pollution.

Geomorphic Evolution of Radial Sand Ridges in the South Yellow Sea Observed from Satellites

The radial sand ridges consist of more than 70 sand ridges that are spread out radially on the continental shelf of the South Yellow Sea. As a unique geomorphological feature in the world, its evolution process and characteristics are crucial to marine resource management and ecological protection. Based on the multi-source remote sensing image data from 1979 to 2019, three types of geomorphic feature lines, artificial coastlines, waterlines, and sand ridge lines were extracted. Using the GIS sequence analysis method (Digital Shoreline Analysis System (DSAS), spatial overlay analysis, standard deviational ellipse method), the evolution characteristics of the shoreline, exposed tidal flats, and underwater sand ridges from land to sea were interpreted. The results demonstrate that: (1) The coastline has been advancing towards the sea with a maximum advance rate of 348.76 m/a from Wanggang estuary to Xiaoyangkou Port. (2) The exposed tidal flats have decreased by 1484 km2 including the reclaimed area of 1414 km2 and showed a trend of erosion in the north around Xiyang channel and deposition in the southeast around the Gaoni and Jiangjiasha areas. (3) The overall sand ridge lines showed a trend of gradually moving southeast (135°), and the moving distance is nearly 4 km in the past 40 years. In particular, the sand ridge of Tiaozini has moved 11 km southward, while distances of 8 km for Liangyuesha and 5 km for Lengjiasha were also observed. For the first time, this study quantified the overall migration trend of the RSRs. The imbalance of the regional tidal wave system may be one of the main factors leading to the overall southeastward shift of the radiation sandbanks.

Characteristics and influencing factors of Cretaceous reservoir in eastern depression of North Yellow Sea basin

Cretaceous is the key exploration target layer in the eastern depression of North Yellow Sea basin, which has a good prospect for oil and gas exploration. Its huge oil and gas resource potential has attracted great attention from petroleum geologists. In this study, the main rock types, reservoir space types, petrophysical characteristics and main controlling factors of Cretaceous reservoir are studied through core observation, thin section identification, petrophysical analysis and scanning electron microscope observation. The results indicate that the main rock types of Cretaceous reservoir in the eastern depression of North Yellow Sea basin are lithic arkose, feldspar lithic sandstone, some feldspar sandstone and a small amount of lithic sandstone. The average porosity is 6.9%, and the average permeability is 0.46 × 10−3 μm, so Cretaceous reservoir in the study area has poor petrophysical characteristics and belongs to low porosity and low permeability reservoir. Cretaceous reservoirs in the study area mainly develop in secondary pores, which are dominated by dissolution pores (including intragranular pores, intercrystalline pores and cleavage pores), followed by fractures. The main factors affecting petrophysical characteristics of Cretaceous reservoir in the study area are provenance properties, sedimentation, diagenesis (including compaction, cementation and dissolution) and tectonism. The provenance properties and sedimentation are the prerequisite conditions affecting petrophysical characteristics. Petrophysical characteristics of feldspar sandstone of Cretaceous reservoir in the study area and lithic arkose are better than that of feldspar lithic sandstone. Both compaction and cementation reduce the porosity and permeability of Cretaceous reservoir in the study area and make petrophysical characteristics become poor, whereas the dissolution and tectonism play an important role in improving petrophysical characteristics of Cretaceous reservoir.

Spatial Distribution of Sound Scattering Layer and Density Estimation of Euphausia pacifica in the Center of the Yellow Sea Bottom Cold Water Determined by Hydroacoustic Surveying

The Yellow Sea Bottom Cold Water (YSBCW) refers to seawater with a water temperature of 10 °C or less found at the bottom of the center of the Yellow Sea. The spatiotemporal variability of the YSBCW directly affects the distribution of organisms in the marine ecosystem. In this study, hydroacoustic and net surveys were conducted in April (spring) to understand the spatial distribution of the sound scattering layer (SSL) and estimate the density of Euphausia pacifica (E. pacifica) in the YSBCW. Despite the shallow water in the YSBCW region, E. pacifica formed an SSL, which was distributed near the bottom during the daytime; it showed a diel vertical migration (DVM) pattern of movement toward the surface during the nighttime. The mean upward and downward swimming speeds around sunset and sunrise were approximately 0.6 and 0.3–0.4 m/min, respectively. The E. pacifica density was estimated in the central, western, and eastern regions; the results were approximately 15.8, 1.3, and 10.3 g/m2, respectively, indicating significant differences according to region. The results revealed high-density distributions in the central and eastern regions related to the water temperature structure, which differs regionally in the YSBCW area. Additional studies are needed regarding the spatial distribution of E. pacifica in the YSBCW and its relationship with various ocean environmental parameters according to season. The results of this study contribute to a greater understanding of the structure of the marine ecosystem in the YSBCW.