scholarly journals In vitro evaluation of cytomechanics of osteoblast and fibroblast cell line for enhanced peri-implant bone and soft tissue engineering

2018 ◽  
Vol 29 ◽  
pp. 61-61
Author(s):  
Santosh Y. Nelogi ◽  
Maheshwari Nelogi
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
In Vitro Evaluation ◽  
Soft Tissue Engineering

scholarly journals Fiber-Based chitosan tubular scaffolds for soft tissue engineering: Fabrication and in vitro evaluation

2005 ◽  
Vol 10 (4) ◽  
pp. 449-453 ◽  
Author(s):  
Aijun Wang ◽  
Qiang Ao ◽  
Wenling Cao ◽  
Chang Zhao ◽  
Yandao Gong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
In Vitro Evaluation ◽  
Soft Tissue Engineering

Preparation of Electrospun PLA Nanofiber Scaffold and the Evaluation In Vitro

2005 ◽  
Vol 288-289 ◽  
pp. 139-142 ◽  
Author(s):  
Xian Tao Wen ◽  
Hong Song Fan ◽  
Yan Fei Tan ◽  
H.D. Cao ◽  
H. Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
High Surface ◽  
Volume Ratio ◽  
Electrospinning Process ◽  
High Porosity ◽  
Electrospun Scaffold ◽  
Nanofiber Scaffold ◽  
Soft Tissue Engineering

A electrospinning process to prepare soft tissue engineering scaffold was introduced in this study. This kind of scaffold was composed with ultrathin fiber and characterized with high porosity, well-interconnected pores and high surface-to-volume ratio. Biodegradable polylaticacid (PLA) was used to spin the scaffold and the scaffold was evaluated in vitro by analysis the microscopic structure, porosity, mechanical property, especially cytocompatibility. The results indicated that the electrospun PLA scaffold showed good cytocompatibility and the tensile property of electrospun scaffold was similar to human’s soft tissue. It could be expected that the electrospun scaffold would be potential in soft tissue engineering or soft tissue repair.

scholarly journals In vitro and in silico modeling of perfluoroalkyl substances mixture toxicity in an amphibian fibroblast cell line

Chemosphere ◽  
2019 ◽  
Vol 233 ◽  
pp. 25-33 ◽  
Author(s):  
Gary Hoover ◽  
Supratik Kar ◽  
Samuel Guffey ◽  
Jerzy Leszczynski ◽  
Maria S. Sepúlveda
Keyword(s):  
Cell Line ◽  
In Silico ◽  
Mixture Toxicity ◽  
Fibroblast Cell ◽  
Perfluoroalkyl Substances ◽  
Fibroblast Cell Line ◽  
In Silico Modeling

scholarly journals Modeling atrial fibrosis in vitro —Generation and characterization of a novel human atrial fibroblast cell line

FEBS Open Bio ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1210-1218 ◽  
Author(s):  
Stephan R. Künzel ◽  
Johanna S. E. Rausch ◽  
Charlotte Schäffer ◽  
Maximilian Hoffmann ◽  
Karolina Künzel ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Atrial Fibrosis

Fibroblast cell line establishment, cryopreservation and interspecies embryos reconstruction in red panda (Ailurus fulgens)

Zygote ◽  
2009 ◽  
Vol 17 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Yong Tao ◽  
Jianming Liu ◽  
Yunhai Zhang ◽  
Meiling Zhang ◽  
Junshun Fang ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Basic Medium ◽  
Fibroblast Cell Line ◽  
Cell Line Establishment ◽  
Red Panda ◽  
Fetal Bovine ◽  
Ailurus Fulgens

SummaryIn evolution, the red panda (Ailurus fulgens) plays a pivotal role in the higher level phylogeny of arctoides carnivore mammals. The red panda inhabits certain Asian countries only and its numbers are decreasing. Therefore, the development of feasible ways to preserve this species is necessary. Genetic resource cryopreservation and somatic cell nuclear transfer (SCNT) have been used extensively to rescue this endangered species. The present study describes the establishment, for the first time, of a red panda ear fibroblast cell line, which was then cryopreserved, thawed and cultured. Through micromanipulation, interspecies embryos were reconstructed using the cryopreserved–thawed fibroblasts of the red panda as the donor and rabbit oocytes as recipients. A total of 194 enucleated rabbit oocytes were reconstructed with red panda ear fibroblasts; enucleated oocytes were activated without fusion as the control. The results show that the fibroblast cell line was established successfully by tissue culture and then cryopreserved in liquid nitrogen. Supplementation with 20% fetal bovine serum and 8% dimethyl sulphoxide in basic medium facilitated the cryopreservation. The interspecies embryos were successfully reconstructed. The cleavage, morulae and blastocyst rates after in vitro culture were 71, 47 and 23% (31/194), respectively. This study indicated that a somatic cell line could be established and cryopreserved from red panda and that rabbit cytoplast supports mitotic cleavage of the red panda karyoplasts and is capable of reprogramming the nucleus to achieve blastocysts.

scholarly journals Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering

2017 ◽  
Vol 8 ◽  
pp. 204173141774415 ◽  
Author(s):  
Lukas Kessler ◽  
Sandra Gehrke ◽  
Marc Winnefeld ◽  
Birgit Huber ◽  
Eva Hoch ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Adipogenic Differentiation ◽  
Building Blocks ◽  
Fatty Acid Binding ◽  
Angiogenic Potential ◽  
Adipose Tissue Engineering ◽  
Angiogenesis Assay ◽  
Soft Tissue Engineering

In vitro–generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose–derived stem cells (hASCs). ASCs were incorporated into methacrylated gelatin/hyaluronan hydrogels. The gels were photocrosslinked with a lithium phenyl-2,4,6-trimethylbenzoylphosphinate photoinitiator and analyzed for cell viability and adipogenic differentiation of ASCs over a period of 30 days. Additionally, an angiogenesis assay was performed to assess their angiogenic potential. After 24 h, ASCs showed increased viability on composite hydrogels. These results were consistent over 21 days of culture. By induction of adipogenic differentiation, the mature adipocytes were observed after 7 days of culture, their number significantly increased until day 28 as well as expression of fatty acid binding protein 4 and adiponectin. Our scaffolds are promising as building blocks for adipose tissue engineering and allowed long viability, proliferation, and differentiation of ASCs.

Tissue engineering with HaCaT cells and a fibroblast cell line

1999 ◽  
Vol 291 (11) ◽  
pp. 600-605 ◽  
Author(s):  
K. Kehe ◽  
Michael Abend ◽  
K. Kehe ◽  
Roland Ridi ◽  
Ralf Uwe Peter ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Hacat Cells ◽  
Fibroblast Cell Line

COMPARISON BETWEEN IN VITRO CO-CULTURE OF MRC-5 FIBROBLAST CELL LINE WITH DIFFERENT TYPES OF STENTS

2010 ◽  
Author(s):  
ELENA ARELLANO-ORDEN ◽  
FRANCISCO JAVIER SAENZ-CORONILLA ◽  
ANA MONTES-WORBOYS ◽  
SONIA MOLINA-PINELO ◽  
MIGUEL ANGEL DE GREGORIO ARIZA ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Different Types

Zebrafish fin-derived fibroblast cell line: A model for in vitro wound healing

2019 ◽  
Vol 42 (4) ◽  
pp. 573-584 ◽  
Author(s):  
Nathiga Nambi Kalaiselvi Sivalingam ◽  
Abdul Majeed Seepoo ◽  
Taju Gani ◽  
Sivakumar Selvam ◽  
Sahul Hameed Azeez Sait
Keyword(s):  
Wound Healing ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line
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