Preparation of Electrospun PLA Nanofiber Scaffold and the Evaluation In Vitro

2005 ◽  
Vol 288-289 ◽  
pp. 139-142 ◽  
Author(s):  
Xian Tao Wen ◽  
Hong Song Fan ◽  
Yan Fei Tan ◽  
H.D. Cao ◽  
H. Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
High Surface ◽  
Volume Ratio ◽  
Electrospinning Process ◽  
High Porosity ◽  
Electrospun Scaffold ◽  
Nanofiber Scaffold ◽  
Soft Tissue Engineering

A electrospinning process to prepare soft tissue engineering scaffold was introduced in this study. This kind of scaffold was composed with ultrathin fiber and characterized with high porosity, well-interconnected pores and high surface-to-volume ratio. Biodegradable polylaticacid (PLA) was used to spin the scaffold and the scaffold was evaluated in vitro by analysis the microscopic structure, porosity, mechanical property, especially cytocompatibility. The results indicated that the electrospun PLA scaffold showed good cytocompatibility and the tensile property of electrospun scaffold was similar to human’s soft tissue. It could be expected that the electrospun scaffold would be potential in soft tissue engineering or soft tissue repair.

scholarly journals Fiber-Based chitosan tubular scaffolds for soft tissue engineering: Fabrication and in vitro evaluation

2005 ◽  
Vol 10 (4) ◽  
pp. 449-453 ◽  
Author(s):  
Aijun Wang ◽  
Qiang Ao ◽  
Wenling Cao ◽  
Chang Zhao ◽  
Yandao Gong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
In Vitro Evaluation ◽  
Soft Tissue Engineering

Biomineralization of Electrospun Nano-HA/PHB GTR Membrane

2007 ◽  
Vol 330-332 ◽  
pp. 695-698 ◽  
Author(s):  
Dong Hua Guan ◽  
Chun Peng Huang ◽  
Ji Liu ◽  
Kun Tian ◽  
Lin Niu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Electrospinning Process ◽  
Nanometer Scale ◽  
High Porosity ◽  
The Novel ◽  
Mineral Crystal ◽  
Novel Technology ◽  
Air Jet

Poly 3-hydroxybutyrate (PHB) as a kind of polysaccharides has been proved promising for tissue engineering because of its biocompatibility and biodegradability. But its poor mechanical properties and hydrophilicity limit its application. In order to explore a new useful porch to improve the performance of PHB-based GTR membrane, membrane composed of nano-HA / PHB composite was manufactured through the air/jet electrospinning process which can potentially generate nanometer scale diameter fibers and enlarge surface area of materials while maintaining high porosity. Successively, the biomineralization behavior of the membrane in supersaturated calcification solution (SCS) was studied. The Results of this investigation show that the successfully manufactured porous nano-HA/PHB membrane has high activity in SCS and its ability of inducing the formation of mineral crystal in vitro than that of the unfilled PHB membrane. It can be concluded that the addition of nano-HA and the novel technology could improve the performance of the PHB-based GTR membrane.

scholarly journals Basic Potential of Carbon Nanotubes in Tissue Engineering Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Hisao Haniu ◽  
Naoto Saito ◽  
Yoshikazu Matsuda ◽  
Tamotsu Tsukahara ◽  
Yuki Usui ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
High Surface ◽  
High Surface Area ◽  
Volume Ratio ◽  
Medical Application ◽  
Mechanical And Thermal Properties ◽  
Biological Responses

Carbon nanotubes (CNTs) are attracting interest in various fields of science because they possess a high surface area-to-volume ratio and excellent electronic, mechanical, and thermal properties. Various medical applications of CNTs are expected, and the properties of CNTs have been greatly improved for use in biomaterials. However, the safety of CNTs remains unclear, which impedes their medical application. Our group is evaluating the biological responses of multiwall CNTs (MWCNTs)in vivoandin vitrofor the promotion of tissue regeneration as safe scaffold materials. We recently showed that intracellular accumulation is important for the cytotoxicity of CNTs, and we reported the active physiological functions CNTs in cells. In this review, we describe the effects of CNTsin vivoandin vitroobserved by our group from the standpoint of tissue engineering, and we introduce the findings of other research groups.

scholarly journals Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering

2017 ◽  
Vol 8 ◽  
pp. 204173141774415 ◽  
Author(s):  
Lukas Kessler ◽  
Sandra Gehrke ◽  
Marc Winnefeld ◽  
Birgit Huber ◽  
Eva Hoch ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Adipogenic Differentiation ◽  
Building Blocks ◽  
Fatty Acid Binding ◽  
Angiogenic Potential ◽  
Adipose Tissue Engineering ◽  
Angiogenesis Assay ◽  
Soft Tissue Engineering

In vitro–generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose–derived stem cells (hASCs). ASCs were incorporated into methacrylated gelatin/hyaluronan hydrogels. The gels were photocrosslinked with a lithium phenyl-2,4,6-trimethylbenzoylphosphinate photoinitiator and analyzed for cell viability and adipogenic differentiation of ASCs over a period of 30 days. Additionally, an angiogenesis assay was performed to assess their angiogenic potential. After 24 h, ASCs showed increased viability on composite hydrogels. These results were consistent over 21 days of culture. By induction of adipogenic differentiation, the mature adipocytes were observed after 7 days of culture, their number significantly increased until day 28 as well as expression of fatty acid binding protein 4 and adiponectin. Our scaffolds are promising as building blocks for adipose tissue engineering and allowed long viability, proliferation, and differentiation of ASCs.

Electrospun hybrid nanofibrous meshes with adjustable performance for potential use in soft tissue engineering

2021 ◽  
pp. 004051752110639
Author(s):  
Ye Qi ◽  
Huiyuan Zhai ◽  
Yaning Sun ◽  
Hongxing Xu ◽  
Shaohua Wu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Soft Tissue ◽  
Fiber Diameter ◽  
Fiber Morphology ◽  
Surface Hydrophilicity ◽  
Nanofibrous Scaffolds ◽  
Soft Tissue Engineering ◽  
The Mean

Electrospun nanofibrous scaffolds have gained extensive attention in the fields of soft tissue engineering and regenerative medicine. In this study, a series of biodegradable nanofibrous meshes were fabricated by electrospinning poly(ε-caprolactone) (PCL) and poly( p-dioxanone) (PPDO) blends with various mass ratios. All the as-developed PCL/PPDO nanofibrous meshes possessed smooth and highly aligned fiber morphology. The mean fiber diameter was 521.5 ± 76.6 nm for PCL meshes and 485.8 ± 88.9 nm for PPDO meshes, and the mean fiber diameter seemed to present a decreasing tendency with the increasing of the PPDO component. For pure PCL meshes, the contact angle was about 117.5 ± 1.6°, the weight loss ratio was roughly 0.2% after 10 weeks of degradation, and the tensile strength was 41.2 ± 2.3 MPa in the longitudinal direction and 4.2 ± 0.1 MPa in the transverse direction. It was found that the surface hydrophilicity and in vitro degradation properties of PCL/PPDO meshes apparently increased, but the mechanical properties of PCL/PPDO meshes obviously decreased when more PPDO component was introduced. The biological tests showed that 4:1 PCL/PPDO nanofibrous meshes and 1:1 PCL/PPDO nanofibrous meshes could obviously promote the adhesion and proliferation of human adipose derived mesenchymal stem cells more than pure PCL and PPDO meshes and 1:4 PCL/PPDO meshes. The results demonstrated that it is feasible to adjust the surface hydrophilicity, degradation profile, and mechanical properties as well as biological properties of as-obtained nanofibrous meshes by blending PCL and PPDO components. This study provides meaningful reference and guidance for the design and development of PCL/PPDO hybrid nanofibrous scaffolds for soft tissue engineering research and application.

Fabrication, characterization and in vitro cell study of gelatin-chitosan scaffolds: New perspectives of use of aloe vera and snail mucus for soft tissue engineering

2019 ◽  
Vol 234 ◽  
pp. 268-280
Author(s):  
Daniel Enrique López Angulo ◽  
Carlos Eduardo Ambrosio ◽  
Rodrigo Lourenço ◽  
Natalia Juliana Nardelli Gonçalves ◽  
Fabio Sergio Cury ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Aloe Vera ◽  
Chitosan Scaffolds ◽  
Soft Tissue Engineering ◽  
Cell Study

scholarly journals Nanofibres made from biocompatible and biodegradable polymers, with potential application as medical textiles

2018 ◽  
Vol 69 (01) ◽  
pp. 55-58 ◽  
Author(s):  
SUBTIRICA ADRIANA-IOANA ◽  
BANCIU CRISTINA ANTONELA ◽  
CHIVU ANDREEA ANA-MARIA ◽  
LAURENTIU CHRISTIAN DINCA
Keyword(s):  
Biodegradable Polymers ◽  
Textile Industry ◽  
High Surface ◽  
High Surface Area ◽  
Volume Ratio ◽  
Electrospun Nanofibers ◽  
Electrospinning Process ◽  
High Porosity ◽  
Medical Textiles ◽  
Small Pore

An important and growing part of the textile industry is the medical and related healthcare and hygiene sector. Recently, ultrafine fiber webs made from biocompatible and biodegradable polymers have been obtained by the electrospinning process. Their unique properties such as high surface area-to-volume ratio, small pore sizes, high porosity, and the possibility of incorporation therapeutic compounds into the electrospun nanofibers has attracted the researcher’s attention lately. This paper presents the obtaining of PEO and PVA nanofibers.

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