Electrical penetration graphs indicate that tricin is a key secondary metabolite of rice, inhibiting phloem feeding of brown planthopper,Nilaparvata lugens

2015 ◽  
Vol 156 (1) ◽  
pp. 14-27 ◽  
Author(s):  
Zhenfei Zhang ◽  
Baiyuan Cui ◽  
Yang Zhang
Keyword(s):  
Secondary Metabolite ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Electrical Penetration Graphs ◽  
Phloem Feeding

RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Transcript Level ◽  
Nilaparvata Lugens ◽  
The Body ◽  
Double Stranded Rna ◽  
Saccharopine Dehydrogenase ◽  
Body Weights ◽  
Nilaparvata Lugens Stål ◽  
Phloem Feeding ◽  
Wing Deformation

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.

Screening of rice genotypes for resistance to the brown planthopper, Nilaparvata lugens Stål

2012 ◽  
Vol 40 (4) ◽  
pp. 502-508 ◽  
Author(s):  
M. P. Ali ◽  
Salem S. Alghamdi ◽  
M. A. Begum ◽  
A. B. M. Anwar Uddin ◽  
M. Z. Alam ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Genotypes

Molecular characterization of insulin‐like peptides in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

2020 ◽  
Vol 29 (3) ◽  
pp. 309-319 ◽  
Author(s):  
W.‐H. Xue ◽  
Y.‐L. Liu ◽  
Y.‐Q. Jiang ◽  
S.‐F. He ◽  
Q.‐Q. Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Brown Planthopper ◽  
Nilaparvata Lugens
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