Entomopathogen Beauveria bassiana as an environmentally friendly alternative for control of brown planthopper (Nilaparvata lugens) and rice black bug (Scotinophara coarctata)

Beauveria bassiana fungi and neem extract as botanical insecticide can be combined to get the synergizing effect to control brown planthopper (BPH). This study was conducted to determine the best combination between the spore density of B. bassiana and concentration of neem extract to control BPH effectively. The two factorial treatment consisted concentration of B. bassiana (106 Colony Forming Unit (CFU) and 107 CFU) and concentration of neem extract (5%, 10%, 15%), and control. The treatments were assigned in Completely Randomized Design with three replications. The data were analyzed using ANOVA and LSD at 5%. The test was conducted in vitro and bio assay of testing insects, BPH, was conducted using dipping method. B. bassiana was suitable with neem extract with T value ranged from 78.58- 90.38. The mortality of BPH occurred on day 5 after application. The highest mortality (91.67%) occurred on the treatment with B. bassiana 107 CFU + neem extract 10%. The shortest LT50 was found at the treatment of B. bassiana 106 CFU+ neem extract 15% (2.74 days) but not significantly different from B. bassiana 107 CFU + neem extract 15% (2.76 days). B. bassiana spores combined with neem extract are assumed to speed up the mortality of BPH by increasing the concentration.

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Growth of Beauveria bassiana Combined with MIPC Insecticide and Its Efficacy to Control the Brown Planthopper Nilaparvata lugens

Research Journal of Life Science ◽

10.21776/ub.rjls.2020.007.01.2 ◽

2020 ◽

Vol 7 (1) ◽

pp. 9-18

Author(s):

Aminudin Afandhi ◽

Yayan Atma Kunjana ◽

Fery Abdul Choliq ◽

Amin Setyo Leksono

Keyword(s):

Beauveria Bassiana ◽

Rice Field ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Sublethal Concentration ◽

Plant Diseases ◽

Vegetative Phase ◽

Significant Difference ◽

Field Efficacy ◽

Development Laboratory

The objectives of this study were to evaluate the efficacy of MIPC to control the brown planthopper Nilaparvata lugens, one of the most damaging pests of rice in the field and determine the effect of various of its doses on the growth of the fungus B. bassiana. The research was carried out in a rice field and in the Biological Agents Development Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Brawijaya University, Indonesia. The field efficacy test of the MIPC insecticide was conducted by applying 0.5-, 1.0-, 1.5-, and 2.0-kg/ha doses. The insecticidal compatibility test of MIPC with B. bassiana was conducted in 0.5-, 1.0-, 1.5-, and 2.0-g/L doses of MIPC. Application of MIPC insecticide at 0.5-, 1.0-, 1.5-, and 2.0-kg/ha doses had no significant difference in reducing N. lugens population in the vegetative phase (28 to 70 days after planting). The MIPC doses of 1.0, 1.5, and 2.0 g/L were highly toxic or incompatible with the growth of B. bassiana. Meanwhile, a sublethal concentration of MIPC insecticide of 0.5 g/L was non-toxic to B. bassiana. Therefore, this study recommends the use of 0.5 g/L MIPC combined with B. bassiana fungi.

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Sublethal effects of Beauveria bassiana sensu lato isolate NJBb2101 on biological fitness and insecticide sensitivity of parental and offspring generations of brown planthopper, Nilaparvata lugens

Biological Control ◽

10.1016/j.biocontrol.2018.02.007 ◽

2018 ◽

Vol 121 ◽

pp. 44-49 ◽

Cited By ~ 2

Author(s):

Yunchao Wang ◽

Na Yu ◽

Fuhua Chu ◽

Shakal Khan Korai ◽

Zewen Liu

Keyword(s):

Beauveria Bassiana ◽

Sublethal Effects ◽

Brown Planthopper ◽

Nilaparvata Lugens

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Development of thermotolerant isolates of Beauveria bassiana (Bals.-Criv.) Vuill. with ethyl methanesulfonate

Journal of Plant Protection Research ◽

10.1515/jppr-2017-0046 ◽

2018 ◽

Vol 0 (0) ◽

Author(s):

Yanisa Wongwanich ◽

Payorm Cobelli ◽

Duangkamon Boonchuay ◽

Teerada Wangsomboondee

Keyword(s):

High Temperature ◽

Beauveria Bassiana ◽

Brown Planthopper ◽

Fungal Growth ◽

Nilaparvata Lugens ◽

Ethyl Methanesulfonate ◽

Wild Type ◽

Pest Insects ◽

Increasing Temperature

AbstractBeauveria bassianais an entomopathogenic fungus that is widely used in Thailand to control pest insects. However, the increasing temperature has influenced the insect control efficiency of the fungus. Therefore, determination of thermotolerant isolates ofB. bassianathat can grow and remain pathogenic at higher temperatures than its current optimum temperature may be a better way to control pest insects in a high temperature environment. Three isolates ofB. bassianaobtained from the Rice Department, Thailand were selected for mutagenesis using ethyl methanesulfonate (EMS) with subsequent screening at high temperatures (33 and 35°C). In addition, the recovery of fungal growth after exposure to a high temperature for a period of time (5–15 days) and then transferring to 25°C was evaluated. No isolates were found that grew at 35°C but one mutant isolate (BCNT002MT) produced larger diameter colonies and more spores than the corresponding wild type (WT) at 33°C. Growth and spore production of the BCNT002MT isolate were greater than its WT when incubated at 25°C for 14 days following exposure to 33°C for 7 days. In addition, the spore germination level (%) of BCNT002MT was significantly higher than its WT during culture at 25°C after prior exposure to 33°C for 5, 10 and 15 days. The pathogenicity against the brown planthopper,Nilaparvata lugens(Stål), of this mutant isolate was also prominent.

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Efektifitas aplikasi Beauveria bassiana sebagai upaya pengendalian wereng batang coklat dan walang sangit pada tanaman padi di Desa Campursari Kecamatan Bulu Kabupaten Temanggung

Journal of Agro Complex ◽

10.14710/joac.2.1.12-18 ◽

2018 ◽

Vol 2 (1) ◽

pp. 12

Author(s):

Tri Purwaningsih ◽

Budi Adi Kristanto ◽

Karno Karno

Keyword(s):

Plant Height ◽

Beauveria Bassiana ◽

Brown Planthopper ◽

Environmentally Friendly ◽

Grain Production ◽

Rice Plants ◽

Randomized Design ◽

Rice Bug ◽

The Village ◽

G 14

Beauveria bassiana is one of the pathogens that can be used as an environmentally friendly bio pesticide, as it does not produce harmful residues and does not cause pest resistance. This research was conducted in the village of Campursari village, District of Bulu, Temanggung Regency. The purpose of this research was assess the effectiveness of Beauveria bassiana application in order to suppress the population of brown planthopper and adult rice bug in rice plants and to find out rice plants production. The research used factorial randomized design which consists of two treatments. The first treatment was the application of Beauveria bassiana with controlled doses by 0, 100, 150 and 200 g/14 l. The second treatment was the application interval between 7, 10, and 14 days. The parameters observed were brown planthopper populations, adult rice bug population, plant height, number of tillers and grain production. The results showed that application with doses of 200 g/14 l and 7 day interval application was effective to suppress population of brown planthopper and adult rice bug and also saved grain production by 1,20 kg to 7,02 kg/m2 depending on application dosage. Keywords : planthopper, adult rice bug, Beauveria, doses, application interval

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Beauveria bassiana sensu lato granules for management of brown planthopper, Nilaparvata lugens in rice

BioControl ◽

10.1007/s10526-014-9632-5 ◽

2014 ◽

Vol 60 (2) ◽

pp. 263-270 ◽

Cited By ~ 4

Author(s):

Se Jin Lee ◽

Jeong Seon Yu ◽

Yu-Shin Nai ◽

Bruce L. Parker ◽

Margaret Skinner ◽

...

Keyword(s):

Beauveria Bassiana ◽

Brown Planthopper ◽

Nilaparvata Lugens

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PATOGENISITAS Beauveria bassiana STRAIN STGD 7(14)2 DAN STGD 5(14)2 TERHADAP WERENG COKLAT (Nilaparvata lugens STÅL)

AL-Kauniyah Jurnal Biologi ◽

10.15408/kauniyah.v11i2.6694 ◽

2018 ◽

Vol 11 (2) ◽

pp. 122-132

Author(s):

Yadi Suryadi ◽

Wartono Wartono ◽

Dwi Ningsih Susilowati ◽

Puji Lestari ◽

Cyntia Nirmalasari ◽

...

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Internal Transcribed Spacer ◽

Insect Pests ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Its Rdna ◽

Stink Bug ◽

West Java ◽

Nilaparvata Lugens Stål

AbstrakBeauveria spp. merupakan salah satu jamur entomopatogen yang dapat digunakan sebagai agen biokontrol terhadap berbagai serangga hama. Tujuan penelitian ini adalah untuk menguji patogenitas Beauveria spp. terhadap serangga wereng coklat (Nilaparvata lugens Stål). Isolat-isolat Beauveria spp. diisolasi dari serangga walang sangit dari Situ Gede, Bogor, Jawa Barat. Identifikasi jamur dilakukan berdasarkan analisis data sekuen dari daerah Internal Transcribed Spacer (ITS) rDNA. Hasil uji menunjukkan bahwa patogenisitas isolat Stgd 5(14)2 dan Stgd 7(14)2 menghasilkan tingkat mortalitas 100% terhadap N. lugens. Isolat Stgd 5(14)2 dan Stgd 7(14)2 memiliki nilai LT50 yang rendah, danmenyebabkan kematian yang cepat terhadap wereng coklat. Hasil analisis filogenetik menunjukkan bahwa sekuen Stgd 5(14)2 dan Stgd 7(14)2 termasuk ke dalam spesies Beauveria bassiana s.str. Hasil studi ini merupakan kajian awal terhadap patogenisitas jamur entomopatogen B. bassiana terhadap wereng coklat, dan distribusinya di pertanaman padi Situ Gede, Jawa Barat.Abstract Beauveria spp. is one of the entomopathogenic fungi that can be used as biocontrol agents against various insect pests, including brown planthopper (Nilaparvata lugens Stal). This study aimed to test the pathogenicity of Beauveria spp. against N. lugens. Beauveria spp. were isolated from the rice stink bug insects, collected from Situ Gede, Bogor, West Java. Fungal identification was carried out based on the internal transcribed spacer (ITS) rDNA analysis. The pathogenicity assay revealed that Stgd 5(14)1 and Stgd 7(14)2 isolates were virulent against N. lugens, with a mortality of 100%. The LT50 (median lethal time) determination, indicated that Stgd 5(14)2 and Stgd 7(14)2 isolates had low value indicating a faster mortality in brown planthopper insects. Phylogenetic analysis showed that the sequences of Stgd 5(14)2 and Stgd 7(14)2 belong to Beauveria bassiana s.str. This is the preliminary pathogenicity trial of entomopathogenic fungi B. bassiana against brown planthopper and their distribution in rice-growing, Situ Gede area in West Java.

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