Expression of Oct-4, SOX-2, and MYC in dental papilla cells and dental follicle cells during in-vivo tooth development and in-vitro co-culture

2014 ◽  
Vol 122 (4) ◽  
pp. 251-258 ◽  
Author(s):  
Zhengjun Peng ◽  
Lu Liu ◽  
Xi Wei ◽  
Junqi Ling
Keyword(s):  
Tooth Development ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Dental Papilla ◽  
Dental Papilla Cells

scholarly journals Comparison of Odontogenic Differentiation of Human Dental Follicle Cells and Human Dental Papilla Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e62332 ◽  
Author(s):  
Lijuan Guo ◽  
Jie Li ◽  
Xiangchen Qiao ◽  
Mei Yu ◽  
Wei Tang ◽  
...  
Keyword(s):  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Dental Papilla ◽  
Odontogenic Differentiation ◽  
Dental Papilla Cells

scholarly journals HDAC6 Regulates the Fusion of Autophagosome and Lysosome to Involve in Odontoblast Differentiation

2020 ◽  
Vol 8 ◽  
Author(s):  
Yunyan Zhan ◽  
Haisheng Wang ◽  
Lu Zhang ◽  
Fei Pei ◽  
Zhi Chen
Keyword(s):  
Tooth Development ◽  
Expression Patterns ◽  
Autophagic Flux ◽  
Differentiation Capacity ◽  
Odontoblast Differentiation ◽  
Dental Papilla ◽  
Odontoblastic Differentiation ◽  
Dental Papilla Cells

Odontoblast differentiation is an important process during tooth development in which pre-odontoblasts undergo elongation, polarization, and finally become mature secretory odontoblasts. Many factors have been found to regulate the process, and our previous studies demonstrated that autophagy plays an important role in tooth development and promotes odontoblastic differentiation in an inflammatory environment. However, it remains unclear how autophagy is modulated during odontoblast differentiation. In this study, we found that HDAC6 was involved in odontoblast differentiation. The odontoblastic differentiation capacity of human dental papilla cells was impaired upon HDAC6 inhibition. Moreover, we found that HDAC6 and autophagy exhibited similar expression patterns during odontoblast differentiation both in vivo and in vitro; the expression of HDAC6 and the autophagy related proteins ATG5 and LC3 increased as differentiation progressed. Upon knockdown of HDAC6, LC3 puncta were increased in cytoplasm and the autophagy substrate P62 was also increased, suggesting that autophagic flux was affected in human dental papilla cells. Next, we determined the mechanism during odontoblastic differentiation and found that the HDAC6 substrate acetylated-Tubulin was up-regulated when HDAC6 was knocked down, and LAMP2, LC3, and P62 protein levels were increased; however, the levels of ATG5 and Beclin1 showed no obvious change. Autophagosomes accumulated while the number of autolysosomes was decreased as determined by mRFP-GFP-LC3 plasmid labeling. This suggested that the fusion between autophagosomes and lysosomes was blocked, thus affecting the autophagic process during odontoblast differentiation. In conclusion, HDAC6 regulates the fusion of autophagosomes and lysosomes during odontoblast differentiation. When HDAC6 is inhibited, autophagosomes can't fuse with lysosomes, autophagy activity is decreased, and it leads to down-regulation of odontoblastic differentiation capacity. This provides a new perspective on the role of autophagy in odontoblast differentiation.

Formation of bone-like tissue by dental follicle cells co-cultured with dental papilla cells

2010 ◽  
Vol 342 (2) ◽  
pp. 221-231 ◽  
Author(s):  
Yudi Bai ◽  
Yuxiang Bai ◽  
Kenichi Matsuzaka ◽  
Sadamitsu Hashimoto ◽  
Eitoyo Kokubu ◽  
...  
Keyword(s):  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Dental Papilla ◽  
Dental Papilla Cells

scholarly journals RORα Regulates Odontoblastic Differentiation and Mediates the Pro-Odontogenic Effect of Melatonin on Dental Papilla Cells

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1098
Author(s):  
Jun Kang ◽  
Haoling Chen ◽  
Fuping Zhang ◽  
Tong Yan ◽  
Wenguo Fan ◽  
...  
Keyword(s):  
Tooth Development ◽  
Critical Role ◽  
Orphan Receptor ◽  
Dental Papilla ◽  
Odontoblastic Differentiation ◽  
Alp Activity ◽  
Matrix Mineralisation ◽  
Dental Papilla Cells

Dental papilla cells (DPCs), precursors of odontoblasts, are considered promising seed cells for tissue engineering. Emerging evidence suggests that melatonin promotes odontoblastic differentiation of DPCs and affects tooth development, although the precise mechanisms remain unknown. Retinoid acid receptor-related orphan receptor α (RORα) is a nuclear receptor for melatonin that plays a critical role in cell differentiation and embryonic development. This study aimed to explore the role of RORα in odontoblastic differentiation and determine whether melatonin exerts its pro-odontogenic effect via RORα. Herein, we observed that RORα was expressed in DPCs and was significantly increased during odontoblastic differentiation in vitro and in vivo. The overexpression of RORα upregulated the expression of odontogenic markers, alkaline phosphatase (ALP) activity and mineralized nodules formation (p < 0.05). In contrast, odontoblastic differentiation of DPCs was suppressed by RORα knockdown. Moreover, we found that melatonin elevated the expression of odontogenic markers, which was accompanied by the upregulation of RORα (p < 0.001). Utilising small interfering RNA, we further demonstrated that RORα inhibition attenuated melatonin-induced odontogenic gene expression, ALP activity and matrix mineralisation (p < 0.01). Collectively, these results provide the first evidence that RORα can promote odontoblastic differentiation of DPCs and mediate the pro-odontogenic effect of melatonin.

Comparison of human dental follicle cells (DFCs) and stem cells from human exfoliated deciduous teeth (SHED) after neural differentiation in vitro

2009 ◽  
Vol 14 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Christian Morsczeck ◽  
Florian Völlner ◽  
Michael Saugspier ◽  
Caroline Brandl ◽  
Torsten Eugen Reichert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Differentiation ◽  
Follicle Cells ◽  
Deciduous Teeth ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Human Exfoliated Deciduous Teeth

Cementum matrix formation in vivo by cultured dental follicle cells

Bone ◽  
2002 ◽  
Vol 31 (5) ◽  
pp. 606-611 ◽  
Author(s):  
K Handa ◽  
M Saito ◽  
M Yamauchi ◽  
T Kiyono ◽  
S Sato ◽  
...  
Keyword(s):  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

Human dental follicle cells acquire cementoblast features under stimulation by BMP-2/-7 and enamel matrix derivatives (EMD) in vitro

2007 ◽  
Vol 329 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Philippe Kémoun ◽  
Sara Laurencin-Dalicieux ◽  
Jacqueline Rue ◽  
Jean-Christophe Farges ◽  
Isabelle Gennero ◽  
...  
Keyword(s):  
Follicle Cells ◽  
Dental Follicle ◽  
Enamel Matrix ◽  
Dental Follicle Cells ◽  
Enamel Matrix Derivatives ◽  
Matrix Derivatives

scholarly journals A two-step strategy for neuronal differentiation in vitro of human dental follicle cells

2009 ◽  
Vol 77 (5) ◽  
pp. 433-441 ◽  
Author(s):  
Florian Völlner ◽  
Wolfgang Ernst ◽  
Oliver Driemel ◽  
Christian Morsczeck
Keyword(s):  
Neuronal Differentiation ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells
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