scholarly journals PM 7/46 (3) Lecanosticta acicola (formerly Mycosphaerella dearnessii ), Dothistroma septosporum (formerly Mycosphaerella pini ) and Dothistroma pini

EPPO Bulletin ◽  
2015 ◽  
Vol 45 (2) ◽  
pp. 163-182 ◽  
Keyword(s):  
Lecanosticta Acicola ◽  
Dothistroma Pini ◽  
Dothistroma Septosporum

scholarly journals Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 362
Author(s):  
Colin L. Myrholm ◽  
Bradley D. Tomm ◽  
Renate Heinzelmann ◽  
Nicolas Feau ◽  
Richard C. Hamelin ◽  
...  
Keyword(s):  
Lamp Assay ◽  
Isothermal Amplification ◽  
Pine Needle ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Lecanosticta Acicola ◽  
Dothistroma Pini ◽  
Biology Laboratory ◽  
Dothistroma Septosporum ◽  
Selection Of

A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.

New country and regional records of the pine needle blight pathogens Lecanosticta acicola , Dothistroma septosporum and Dothistroma pini

2018 ◽  
Vol 48 (5) ◽  
pp. e12440 ◽  
Author(s):  
M. S. Mullett ◽  
K. Adamson ◽  
H. Bragança ◽  
T. S. Bulgakov ◽  
M. Georgieva ◽  
...  
Keyword(s):  
Pine Needle ◽  
Lecanosticta Acicola ◽  
Dothistroma Pini ◽  
Dothistroma Septosporum ◽  
Needle Blight

scholarly journals New data on the spread of the invasive species Dothistroma septosporum (Dorogin) M. Morelet in Belarus

2021 ◽  
Vol 66 (2) ◽  
pp. 147-158
Author(s):  
L. A. Golovchenko ◽  
N. G. Dishuk ◽  
S. V. Panteleev ◽  
O. Yu. Baranov
Keyword(s):  
Invasive Species ◽  
Molecular Genetic ◽  
Botanical Garden ◽  
Pine Trees ◽  
The Republic ◽  
Forest Nurseries ◽  
Dothistroma Needle Blight ◽  
Dothistroma Pini ◽  
Dothistroma Septosporum ◽  
Needle Blight

Red band needle blight, or Dothistroma needle blight is one of the most common and harmful diseases of pine. The causative agents of the disease are pathogenic micromycetes Dothistroma septosporum (Dorogin) M. Morelet and Dothistroma pini Hulbary. Dothistroma needle blight was firstly detected in Belarus in 2012 year, but till now information about this disease in the republic is fragmentary. The article presents the results of a survey of different pine trees, carried out in the period 2016–2020 years in botanical and dendrological gardens, forest nurseries and mini-arboretums at forestry enterprises, urban stands, nurseries of decorative plants, garden centers, for the presence of Dothistroma needle blight. The species identification of the causative agent of the disease was carried out by mycological and molecular genetic methods. In this study, Dothistroma needle blight was revealed on individual trees of Pinus mugo, P. nigra and P. ponderosa in the stands of the Central Botanical Garden of the NAS of Belarus, the dendrological garden of the Glubokoe experimental forestry enterprise, in the nurseries of decorative plants in the Grodno and Minsk regions. In the collected samples of needles, the invasive species Dothistroma septosporum was identified. The frequency of occurrence of the pathogen was 4.8–7.2 %, the proportion of observation sites in which this disease was detected at 60 %. The detection of Dothistroma needle blight on pine trees, mainly on planting material imported from abroad, indicates a transboundary route of D. septosporum entering the country. Analysis of literature data indicates the potential danger of Dothistroma needle blight for pine stands in the republic, which in turn requires the organization of regular monitoring of the disease and the development of methods to limit the spread of D. septosporum in the republic.

scholarly journals Dothistroma septosporum Not Detected in Pinus sylvestris Seed Trees from Investigated Stands in Southern Poland

Forests ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1323
Author(s):  
Pola Wartalska ◽  
Tomasz Oszako ◽  
Sławomir Bakier ◽  
Lassaâd Belbahri ◽  
Tadeusz Malewski ◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Black Pine ◽  
Southern Europe ◽  
First Report ◽  
Seed Collection ◽  
Southern Poland ◽  
Pine Stands ◽  
Lecanosticta Acicola ◽  
Dothistroma Septosporum ◽  
Propagation Material

In recent years, the decline of pine stands in Europe, including Poland, has been caused by the emerging needle pathogen Dothistroma septosporum. Although this fungus appears to preferentially infect Pinus pini, P. pinaster or P. radiata in Southern Europe, it has been reported in stands of P. nigra, P. mugo and P. sylvestris from Southern Poland. Our preliminary tests of symptomatic needles of diseased pines, including black pine (P. nigra), showed the presence of both D. septosporum and D. pini—the latter as the first report in Poland. No other endophytic pathogen, i.e., Lecanosticta acicola or Cenangium ferruginosum, were found. More extensive molecular surveying based on β-tub2 amplification of DNA in needle samples from 72 seed trees of P. sylvestris in nine different Forest Districts of Southern Poland did not find the presence of D. septosporum. Our study revealed that the seed trees from which we collected propagation material were free from the pathogen, and its endophytic behavior was not confirmed in our testing. Consequently, these investigated trees of P. sylvestris should be suitable for seed collection and propagation, following the requirements of “good” phytosanitary quality as “pathogen-free” pine seeds used for reforestation.

Author response for "New hosts for Lecanosticta acicola and Dothistroma septosporum in newly established arboreta in Spain"

2020 ◽  
Author(s):  
Nebai Mesanza ◽  
Rosa Raposo ◽  
Margarita Elvira‐Recuenco ◽  
Irene Barnes ◽  
Ariska Nest ◽  
...  
Keyword(s):  
Author Response ◽  
New Hosts ◽  
Lecanosticta Acicola ◽  
Dothistroma Septosporum
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