scholarly journals Revealing G-protein-coupled receptor oligomerization at the single-molecule level through a nanoscopic lens: methods, dynamics and biological function

FEBS Journal ◽  
2015 ◽  
Vol 283 (7) ◽  
pp. 1197-1217 ◽  
Author(s):  
Marco Scarselli ◽  
Paolo Annibale ◽  
Peter J. McCormick ◽  
Shivakumar Kolachalam ◽  
Stefano Aringhieri ◽  
...  
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
Biological Function ◽  
G Protein Coupled Receptor ◽  
Single Molecule Level ◽  
Receptor Oligomerization ◽  
G Protein Coupled

Evidence of G-protein-coupled receptor and substrate transporter heteromerization at a single molecule level

2017 ◽  
Vol 75 (12) ◽  
pp. 2227-2239 ◽  
Author(s):  
Jana Fischer ◽  
Gunnar Kleinau ◽  
Claudia Rutz ◽  
Denise Zwanziger ◽  
Noushafarin Khajavi ◽  
...  
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
G Protein Coupled Receptor ◽  
Single Molecule Level ◽  
G Protein Coupled

scholarly journals Conformational Dynamics of a G Protein-Coupled Receptor at the Single-Molecule Level

2015 ◽  
Vol 108 (2) ◽  
pp. 350a
Author(s):  
Rajan Lamichhane ◽  
Jeffrey J. Liu ◽  
Raymond C. Stevens ◽  
David P. Millar
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
Conformational Dynamics ◽  
G Protein Coupled Receptor ◽  
Single Molecule Level ◽  
G Protein Coupled

Human dipeptidyl peptidase III regulates G-protein coupled receptor-dependent Ca2+ concentration in human embryonic kidney 293T cells

2016 ◽  
Vol 397 (6) ◽  
pp. 563-569 ◽  
Author(s):  
Subhash C. Prajapati ◽  
Ratnakar Singh ◽  
Shyam S. Chauhan
Keyword(s):  
Angiotensin Ii ◽  
G Protein ◽  
Biological Function ◽  
Dipeptidyl Peptidase ◽  
Luciferase Reporter ◽  
G Protein Coupled Receptor ◽  
Intracellular Ca ◽  
First Time ◽  
G Protein Coupled

Abstract The precise biological function of human dipeptidyl peptidase III (hDPP III) is poorly understood. Using luciferase reporter constructs responsive to change in Ca2+ and/or cAMP and Fura 2-AM fluorometric assay, we show a significant decrease in intracellular Ca2+ following hDPP III overexpression and angiotensin II stimulation in angiotensin II type 1 receptor (G-protein coupled receptor, GPCR) expressing HEK293T cells. Silencing the expression of hDPP III by siRNA reversed the effect of hDPP III overexpression with a concomitant increase in Ca2+. These results, for the first time, show involvement of hDPP III in GPCR dependent Ca2+ regulation in HEK293T cells.

scholarly journals Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (12) ◽  
Author(s):  
Rajan Lamichhane ◽  
Jeffrey Liu ◽  
Raymond Pauszek III ◽  
David Millar
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals A Computational Investigation of the Effect of Membrane Curvature on G-Protein Coupled Receptor Oligomerization

2013 ◽  
Vol 104 (2) ◽  
pp. 114a
Author(s):  
Ernesto E. Borrero ◽  
Davide Provasi ◽  
Signe Mathiasen ◽  
Dimitrios Stamou ◽  
Marta Filizola
Keyword(s):  
G Protein ◽  
Membrane Curvature ◽  
Computational Investigation ◽  
G Protein Coupled Receptor ◽  
Receptor Oligomerization ◽  
G Protein Coupled

scholarly journals Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles

Nanoscale ◽  
2020 ◽  
Vol 12 (21) ◽  
pp. 11518-11525 ◽  
Author(s):  
Rachael L. Grime ◽  
Joelle Goulding ◽  
Romez Uddin ◽  
Leigh A. Stoddart ◽  
Stephen J. Hill ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
Single Molecule ◽  
Maleic Acid ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
G Protein Coupled Receptor ◽  
Fluorescence Correlation ◽  
Lipid Particles ◽  
G Protein Coupled

Combining the technologies of encapsulation of GPCRs in SMA lipid particles with fluorescence correlation spectroscopy provides a versatile characterisation platform.

scholarly journals Imaging of G protein-coupled receptors in solid-supported planar membranes at the single molecule level

2008 ◽  
Author(s):  
Iwan Märki ◽  
Marcel Leutenegger ◽  
Matthias Geissbuehler ◽  
Rudolf Robelek ◽  
Eva-Kathrin Sinner ◽  
...  
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
G Protein Coupled Receptors ◽  
Single Molecule Level ◽  
G Protein Coupled ◽  
Planar Membranes
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