scholarly journals Torsional stress in DNA limits collaboration among reverse gyrase molecules

FEBS Journal ◽  
2016 ◽  
Vol 283 (8) ◽  
pp. 1372-1384 ◽  
Author(s):  
Taisaku Ogawa ◽  
Kazuo Sutoh ◽  
Akihiko Kikuchi ◽  
Kazuhiko Kinosita
Keyword(s):  
Torsional Stress ◽  
Reverse Gyrase

scholarly journals A reverse gyrase with an unusual structure. A type I DNA topoisomerase from the hyperthermophile Methanopyrus kandleri is a two-subunit protein

1994 ◽  
Vol 269 (15) ◽  
pp. 11081-11089
Author(s):  
S.A. Kozyavkin ◽  
R. Krah ◽  
M. Gellert ◽  
K.O. Stetter ◽  
J.A. Lake ◽  
...  
Keyword(s):  
Type I ◽  
Reverse Gyrase ◽  
Unusual Structure ◽  
Dna Topoisomerase ◽  
Subunit Protein ◽  
Methanopyrus Kandleri

Optical vortices in twisted optical fibres with torsional stress

2008 ◽  
Vol 10 (9) ◽  
pp. 095007 ◽  
Author(s):  
C N Alexeyev ◽  
A V Volyar ◽  
M A Yavorsky
Keyword(s):  
Optical Vortices ◽  
Optical Fibres ◽  
Torsional Stress

Analysis of DNA cleavage by reverse gyrase from Sulfolobus shibatae B12

1999 ◽  
Vol 260 (1) ◽  
pp. 103-111 ◽  
Author(s):  
Christine Jaxel ◽  
Michel Duguet ◽  
Marc Nadal
Keyword(s):  
Dna Cleavage ◽  
Reverse Gyrase ◽  
Sulfolobus Shibatae

Reverse gyrase is essential for microbial growth at 95 °C

Extremophiles ◽  
2017 ◽  
Vol 21 (3) ◽  
pp. 603-608 ◽  
Author(s):  
Gina L. Lipscomb ◽  
Elin M. Hahn ◽  
Alexander T. Crowley ◽  
Michael W. W. Adams
Keyword(s):  
Microbial Growth ◽  
Reverse Gyrase

scholarly journals Torsional Stress Wave Propagation in a Semi-Infinite Conical Bar

1975 ◽  
Vol 41 (345) ◽  
pp. 1345-1353
Author(s):  
Hiroyuki MATSUMOTO ◽  
Eiichiro TSUCHIDA ◽  
Saburo MIYAO ◽  
Naomasa TUNADA
Keyword(s):  
Wave Propagation ◽  
Stress Wave ◽  
Stress Wave Propagation ◽  
Torsional Stress

Magnetic domain structure of amorphous Fe73.5Si13.5B9Nb3Cu1 wires under torsional stress

2008 ◽  
Vol 103 (7) ◽  
pp. 07E716 ◽  
Author(s):  
B. Hernando ◽  
M. L. Sánchez ◽  
V. M. Prida ◽  
J. D. Santos ◽  
J. Olivera ◽  
...  
Keyword(s):  
Domain Structure ◽  
Magnetic Domain ◽  
Magnetic Domain Structure ◽  
Torsional Stress

scholarly journals The Archaeal Topoisomerase Reverse Gyrase Is a Helix-destabilizing Protein That Unwinds Four-way DNA Junctions

2010 ◽  
Vol 285 (47) ◽  
pp. 36532-36541 ◽  
Author(s):  
Anna Valenti ◽  
Giuseppe Perugino ◽  
Antonio Varriale ◽  
Sabato D'Auria ◽  
Mosè Rossi ◽  
...  
Keyword(s):  
Reverse Gyrase ◽  
Dna Junctions

scholarly journals Investigation of the Warping Torsion of a Press Machine

2020 ◽  
Vol 15 (3) ◽  
pp. 150-161
Author(s):  
Antal Gábor Erdős ◽  
Károly Jármai
Keyword(s):  
Normal Stress ◽  
Cross Section ◽  
Torsional Stress ◽  
Very High ◽  
Open Cross Section

In this article, the investigation of a press machine with 30 tons of pressing weight is presented. The beam of this machine is an I-beam, which has an open cross-section. It is known that this version of cross-section is sensitive to torsional stress. The stress from warping torsion is normal stress, so the opened cross-section is more sensitive to this type of stress. The bimoment that causes normal stress can also be very high, so dealing with this stress is very important.

scholarly journals DNA sequence is a major determinant of tetrasome dynamics

2019 ◽  
Author(s):  
O. Ordu ◽  
A. Lusser ◽  
N. H. Dekker
Keyword(s):  
Dna Sequence ◽  
Dna Sequences ◽  
Dynamic Properties ◽  
Conformational Dynamics ◽  
Magnetic Tweezers ◽  
Torsional Stress ◽  
High Affinity ◽  
Canonical State ◽  
Left And Right ◽  
Dna Wrapping

ABSTRACTEukaryotic genomes are hierarchically organized into protein-DNA assemblies for compaction into the nucleus. Nucleosomes, with the (H3-H4)2 tetrasome as a likely intermediate, are highly dynamic in nature by way of several different mechanisms. We have recently shown that tetrasomes spontaneously change the direction of their DNA wrapping between left- and right-handed conformations, which may prevent torque build-up in chromatin during active transcription or replication. DNA sequence has been shown to strongly affect nucleosome positioning throughout chromatin. It is not known, however, whether DNA sequence also impacts the dynamic properties of tetrasomes. To address this question, we examined tetrasomes assembled on a high-affinity DNA sequence using freely orbiting magnetic tweezers. In this context, we also studied the effects of mono- and divalent salts on the flipping dynamics. We found that neither DNA sequence nor altered buffer conditions affect overall tetrasome structure. In contrast, tetrasomes bound to high-affinity DNA sequences showed significantly altered flipping kinetics, predominantly via a reduction in the lifetime of the canonical state of left-handed wrapping. Increased mono- and divalent salt concentrations counteracted this behaviour. Thus, our study indicates that high-affinity DNA sequences impact not only the positioning of the nucleosome, but that they also endow the subnucleosomal tetrasome with enhanced conformational plasticity. This may provide a means to prevent histone loss upon exposure to torsional stress, thereby contributing to the integrity of chromatin at high-affinity sites.STATEMENT OF SIGNIFICANCECanonical (H3-H4)2 tetrasomes possess high conformational flexibility, as evidenced by their spontaneous flipping between states of left- and right-handed DNA wrapping. Here, we show that these conformational dynamics of tetrasomes cannot be described by a fixed set of rates over all conditions. Instead, an accurate description of their behavior must take into account details of their loading, in particular the underlying DNA sequence. In vivo, differences in tetrasome flexibility could be regulated by modifications of the histone core or the tetrasomal DNA, and as such constitute an intriguing, potentially adjustable mechanism for chromatin to accommodate the torsional stress generated by processes such as transcription and replication.

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