Single‐cell transcriptome profiling reveals molecular heterogeneity in human umbilical cord tissue and culture‐expanded mesenchymal stem cells

FEBS Journal ◽  
2021 ◽  
Author(s):  
Quanlei Wang ◽  
Jinlu Li ◽  
Shengpeng Wang ◽  
Qiuting Deng ◽  
Kuixing Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Transcriptome Profiling ◽  
Molecular Heterogeneity ◽  
Human Umbilical Cord ◽  
Umbilical Cord Tissue ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome ◽  
Expanded Mesenchymal Stem Cells

scholarly journals PB1988 CANCER-ASSOCIATED MECHANISMS OF MESENCHYMAL STEM CELLS REVEALED BY SINGLE-CELL TRANSCRIPTOME ANALYSIS

HemaSphere ◽  
2019 ◽  
Vol 3 (S1) ◽  
pp. 901
Author(s):  
A. Stucky ◽  
X. Chen ◽  
C. Li ◽  
J. cai ◽  
X. Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Transcriptome Analysis ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals Single-cell transcriptome analysis of uncultured human umbilical cord mesenchymal stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shaoyang Zhang ◽  
Jing Yi Wang ◽  
Baojie Li ◽  
Feng Yin ◽  
Huijuan Liu
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Cartilage Growth ◽  
Group 2 ◽  
Cell Transcriptome

AbstractUmbilical cord mesenchymal stem cells (UC-MSCs) have certain advantages over other MSCs and about 300 clinical trials have been registered using UC-MSCs to treat diseases such as osteoarthritis, autoimmune diseases, and degenerative disorders, yet, only limited success has been achieved. One reason is that in vitro expanded UC-MSCs show tremendous heterogeneity and their relationship to in vivo UC-MSCs remains unknown. Here, we investigated freshly isolated, uncultured UC-MSCs by single-cell RNA sequencing (scRNA-seq) and found two populations of UC-MSCs. Although UC-MSCs share many expressed genes and may have the same origin, they can be clearly separated based on differentially expressed genes including CD73 and other markers. Moreover, group 1 MSCs are enriched in expression of genes in immune response/regulatory activities, muscle cell proliferation and differentiation, stemness, and oxidative stress while group 2 MSCs are enriched with gene expression in extracellular matrix production, osteoblast and chondrocytes differentiation, and bone and cartilage growth. These findings suggest that UC-MSCs should be separated right after isolation and individually expanded in vitro to treat different diseases.

scholarly journals Single‐cell transcriptome atlas of human mesenchymal stem cells exploring cellular heterogeneity

2021 ◽  
Vol 11 (12) ◽  
Author(s):  
Zheng Wang ◽  
Chengyan Chai ◽  
Rui Wang ◽  
Yimei Feng ◽  
Lei Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Human Mesenchymal Stem Cells ◽  
Cellular Heterogeneity ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals Single-Cell Transcriptome Analyses Reveal Signals to Activate Dormant Neural Stem Cells

Cell ◽  
2015 ◽  
Vol 161 (5) ◽  
pp. 1175-1186 ◽  
Author(s):  
Yuping Luo ◽  
Volkan Coskun ◽  
Aibing Liang ◽  
Juehua Yu ◽  
Liming Cheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell ◽  
Transcriptome Analyses ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals High-Throughput Single-Cell Transcriptome Profiling of Plant Cell Types

Cell Reports ◽  
2019 ◽  
Vol 27 (7) ◽  
pp. 2241-2247.e4 ◽  
Author(s):  
Christine N. Shulse ◽  
Benjamin J. Cole ◽  
Doina Ciobanu ◽  
Junyan Lin ◽  
Yuko Yoshinaga ◽  
...  
Keyword(s):  
Single Cell ◽  
Plant Cell ◽  
High Throughput ◽  
Transcriptome Profiling ◽  
Cell Types ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

Comparison of different cryopreservation protocols for human umbilical cord tissue as source of mesenchymal stem cells

2019 ◽  
Vol 121 (3) ◽  
pp. 361-367 ◽  
Author(s):  
Basak Isildar ◽  
Serbay Ozkan ◽  
Mahmut Oncul ◽  
Zafer Baslar ◽  
Semih Kaleli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Umbilical Cord Tissue
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