Scanning electron microscopy of the seed coat in the genus Paulownia (Scrophulariaceae)

1993 ◽  
Vol 111 (4) ◽  
pp. 505-511 ◽  
Author(s):  
RADMILA VUJIČIĆ ◽  
D. GRUBIŠIĆ ◽  
R. KONJEVIĆ
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Seed Coat ◽  
Scanning Electron

Taxonomic application of macro and micro morphological characters of seeds in Astragalus L. (Galegeae, Fabaceae) in India

Phytotaxa ◽  
2021 ◽  
Vol 502 (2) ◽  
pp. 191-207
Author(s):  
SHIVANI KASHYAP ◽  
CHANDAN KUMAR SAHU ◽  
ROHIT KUMAR VERMA ◽  
LAL BABU CHAUDHARY
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Seed Coat ◽  
Morphological Plasticity ◽  
Morphological Characters ◽  
Individual Species ◽  
Large Size ◽  
The Individual ◽  
Scanning Electron

Due to large size and enormous morphological plasticity, the taxonomy of the genus Astragalus is very complex and challenging. The identification and grouping of species chiefly based on macromorphological characters become sometimes difficult in the genus. In the present study, the micromorphology of the seeds of 30 species belonging to 14 sections of Astragalus from India has been examined applying scanning electron microscopy (SEM) along with light microscopy (LM) to evaluate their role in identification and classification. Attention was paid to colour, shape, size and surface of seeds. The overall size of the seeds ranges from 1.5–3.2 × 0.8–2.2 mm. The shape of the seeds is cordiform, deltoid, mitiform, orbicular, ovoid and reniform. The colour of seeds varies from brown to blackish-brown to black. Papillose, reticulate, ribbed, rugulate and stellate patterns were observed on the seed coat surface (spermoderm) among different species. The study reveals that the seed coat ornamentations have evolved differently among species and do not support the subgeneric and sectional divisions of the genus. However, they add an additional feature to the individual species, which may help in identification in combination with other macro-morphological features.

scholarly journals Seed morphology and anatomy of Hypericum elegans Steph. ex Willd.

2014 ◽  
Vol 35 (1) ◽  
pp. 15-18
Author(s):  
Piotr Szkudlarz
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Eastern Europe ◽  
Seed Coat ◽  
Central And Eastern Europe ◽  
Seed Morphology ◽  
Seed Sample ◽  
Cell Layers ◽  
Mature Seeds ◽  
Scanning Electron

Abstract Hypericum elegans is a rare perennial distributed primarily in Central and Eastern Europe. Seed morphology and anatomy in H. elegans was studied on the basis of a seed sample from its only locality in Poland. Scanning electron microscopy revealed that the seed coat of mature seeds is composed basically of 3 cell layers: epidermal, subepidermal and sclerenchymatic. They are documented graphically here.

Detailed analysis of Cucurbita pepo seed coat types and structures with scanning electron microscopy

Botany ◽  
2012 ◽  
Vol 90 (11) ◽  
pp. 1161-1169 ◽  
Author(s):  
Jana Murovec ◽  
Kazimir Drašlar ◽  
Borut Bohanec
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Seed Coat ◽  
Cucurbita Pepo ◽  
Fluorescent Microscopy ◽  
Wall Thickening ◽  
Pumpkin Seed ◽  
First Time ◽  
Seed Coats ◽  
Scanning Electron

Discovery of a mutant thin-coated seed phenotype at the end of the 19th century facilitated pumpkin (Cucurbita pepo L.) seed oil production and increased botanic interest in seed coat types and their structures. The main seed coat characteristics were usually analyzed by light and fluorescent microscopy, and more recently, seed coat traits have also been mapped on a C. pepo gene map. The aim of our research was to collect and describe various pumpkin seed types and to analyze, using scanning electron microscopy (SEM), the detailed structure of their seed coats. Seeds of 29 cultivars and landraces were collected and visually evaluated based on seed coat characteristics. Seed samples belonging to different seed types discovered in our collection were transversely sectioned and analyzed by SEM. Twelve seed types were determined, and SEM analysis revealed high variability in their seed coat structures. Using SEM, tissue and cell structures were clearly visible, and novel details of cell and tissue topography were documented. Hypodermal and aerenchyma cells in wild-type seed coats showed fibrous or reticulate secondary cell wall thickening, respectively. In mutant seed types, an absence of different seed coat layers was clearly noted, while the remaining layers were distinctly pronounced. A new completely hull-less seed type was described for the first time. Description of the variability of seed coats in pumpkin was complemented by novel seed coat types, and their structures were analyzed in detail the first time by SEM.

scholarly journals Exomorphic Characters of Seeds of Some Species of Capparaceae Family in Taif, KSA

2019 ◽  
pp. 1-7
Author(s):  
Hatim M. Al-Yasi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Seed Coat ◽  
Seed Shape ◽  
Specific Level ◽  
Coarse Texture ◽  
Wide Range ◽  
Capparis Decidua ◽  
Sem Investigation ◽  
Scanning Electron

Seed exomorphic characters of six species belonging to four genera of family Capparaceae were studied using light microscope (LM) and scanning electron microscopy (SEM) in order to determine the importance of seed features as taxonomic evidence. These species are Cadaba farinosa Forssk, Capparis decidua (Forssk). Edgew, Capparis cartilaginea Decne, Dipterygium glaucum Decne, Maerua crassifolia Forssk and Maerua oblongifolia (Forssk.) A. Rich. The diagnostic characters at level of the general and specific level are shape of seed, size, color, texture and seed coat surface. There is a wide range of variations of seed shape among the studied species. LM revealed that the studied seeds are mostly varying from ovate to globose or cardiac- shape. All of the seeds lack wings except Dipterygium glaucum Decne. and most of the seed is coarse texture. There are six main types of seed surface sculptures revealed by SEM investigation at higher magnifications; reticulate, rugose-striate, winding undulate, ribbed and papillate.

scholarly journals The species of Mentzelia (Loasaceae) in Mexico, part 2: Mentzelia section Bartonia

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
John Schenk ◽  
Carolina Granados Mendoza ◽  
Andres Eduardo Estrada-Castillón
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Species Diversity ◽  
North America ◽  
Species Identification ◽  
Seed Coat ◽  
Western North America ◽  
Distribution Ranges ◽  
Scanning Electron ◽  
Study Species

Background: Mentzelia (Loasaceae) is primarily distributed in western North America. The genus is classified into six monophyletic sections, among which species of section Bartonia are particularly poorly understood. Questions: What species of Mentzelia section Bartonia occur in Mexico? What are the species distribution ranges and what are their defining attributes? Study species: Mentzelia section Bartonia. Methods: Field, herbarium, distribution, and scanning electron microscopy studies were conducted to assess species diversity. Seed coat microsculpture patterns were evaluated to confirm species identities and understand their distributions throughout Mexico. Results: Six species from section Bartonia were recognized, with taxa distributed into either the Chihuahuan or Sonoran deserts. Mentzelia longiloba var. pinacatensis is the only Mexican endemic. Conclusions: Although this work comprehensively addressed the species of section Bartonia in Mexico, two groups will continue to present identification challenges: the M. longiloba and M. mexicana-M. saxicola complexes. Overlapping and variable characters will continue to complicate species identification in the M. longiloba complex, while the late-season loss of primary leaves will obscure species identification between M. mexicana and M. saxicola.

Les graines du genre Digitalis (Scrophulariacea)

1986 ◽  
Vol 64 (7) ◽  
pp. 1389-1396 ◽  
Author(s):  
Gaétan Guignard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Seed Size ◽  
Seed Coat ◽  
Outer Layer ◽  
Scanning Electron

Seeds of most species in the genus Digitalis were studied by scanning electron microscopy, with particular reference to the micromorphology of the cells of the outer layer of the seed coat as well as seed size and weight. Four types of seeds, corresponding to four of the five recognized sections of the genus (Digitalis, Macranthae, Tubiflorae, and Frutescens), were distinguishable. The fifth section, Globiflorae, shows some affinities with Tubiflorae and Frutescens. The taxonomical importance of these seeds is discussed as are similarities between groupings of seeds and phylogeny in this genus.

scholarly journals Post-seminal structure and development of the hemiparasitic plant Escobedia grandiflora (Orobanchaceae)

2019 ◽  
Vol 41 ◽  
pp. e46667
Author(s):  
Edison Cardona Medina ◽  
Marisa Santos ◽  
Rubens Onofre Nodari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Seed Coat ◽  
Root Hair ◽  
Nutritional Value ◽  
Pennisetum Purpureum ◽  
South American ◽  
Cortical Cells ◽  
Plant Stage ◽  
Scanning Electron

Escobedia grandiflora (L.f.) Kuntze is a wild hemiparasitic plant with orange roots. Little is known about the development of initial parasitism with the host, despite the significant value of roots for Central and South American communities. Therefore, this study aimed to characterize post-seminal structure and development of E. grandiflora in Pennisetum purpureum host. To analyze the structure and development of E. grandiflora, seedlings, stems and roots samples were processed and examined under light, confocal and scanning electron microscopy. Escobedia grandiflora seeds are composed of seed coat, perisperm, and embryo. Emergence of the radicle began eleven days after imbibition. Seedlings showed a root hair collar encircling the axis at the root-hypocotyl junction with elongation of internal cortical cells. Seedlings formed haustoria and successfully reached of the host roots 22 days following root emergence. In the root many starch grains were observed, albeit more scarce in the hypocotyl. After 43 days of root emergence, the seedling stage was finished with the formation of the definitive leaves, and star of the plant stage. After 64 days, root ramification, amount of starch, and orange pigmentation increased with formation of haustoria. The developmental pattern of E. grandiflora plants was slow, but the roots grew faster than the stem. Escobedia grandiflora seeds were not endospermic and have limited nutritional value. After root emergence, the young seedling must develop roots and starch storage towards to haustorium formation and attachment to host roots.

scholarly journals Seed coat and fruit surface micromorphology of some Cynoglossum L. (Boraginaceae) species

1970 ◽  
Vol 37 (2) ◽  
pp. 115-119 ◽  
Author(s):  
Oznur Ergen Akcin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Black Sea ◽  
Seed Coat ◽  
Black Sea Region ◽  
Surface Micromorphology ◽  
Surface Ornamentation ◽  
Seed Coats ◽  
Fruit Surface ◽  
Scanning Electron

Fruits and seeds of Cynoglossum creticum Miller, C. officinale L., C. montanum L. and C. glochidiatum Wallich) distributed in the Middle and East Black Sea Region in Turkey were studied by scanning electron microscopy. Some differences were found in seed coats and fruit surfaces. SEM observations of fruit surface were focused on surface ornamentation and glochids. Two types (tuberculate and granulate) and two subtypes (granulate - punctuate and granulate - tuberculate) were observed among the species. Reticulate type of seed coat and detailed subtypes of reticulate types were determined on the basis of ornamentation of the seed coats.     Key words: Cynoglossum, Fruit surface, Seedcoat, SEM, Micromorphology, Boraginaceae doi:10.3329/bjb.v37i2.1716 Bangladesh J. Bot. 37(2): 115-119, 2008 (December)

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