The effects of temperature acclimation on the oxygen consumption and enzyme activity of red and white muscle fibres isolated from the tropical freshwater fish Oreochromis niloticus

1994 ◽  
Vol 44 (6) ◽  
pp. 1033-1043 ◽  
Author(s):  
D. M. Mwangangi ◽  
G. Mutungi
Keyword(s):  
Enzyme Activity ◽  
Oxygen Consumption ◽  
Freshwater Fish ◽  
Oreochromis Niloticus ◽  
White Muscle ◽  
Temperature Acclimation ◽  
Muscle Fibres ◽  
Effects Of Temperature ◽  
Tropical Freshwater

scholarly journals Respiratory Surveillance and Ca2+-ATPase Enzyme Activity Studies of Clarias gariepinus Exposed to Acute Toxicity of Cyanide

2016 ◽  
Vol 50 ◽  
pp. 62-69
Author(s):  
Kadiri Oseni
Keyword(s):  
Enzyme Activity ◽  
Oxygen Consumption ◽  
Acute Toxicity ◽  
Freshwater Fish ◽  
Aquatic Ecosystems ◽  
Clarias Gariepinus ◽  
Potassium Cyanide ◽  
Strong Relationship ◽  
Short Term ◽  
Consumption Index

Potassium cyanide, a highly contaminating and toxic aquatic ecosystems pollutant was investigated for acute toxicity on the freshwater fish Clariasgariepinus. Its effect on the Ca2+ -ATPase activities in the liver, gills, muscle and intestinal tissues and oxygen consumption index was studied. Short-term toxicity test was carried out by static renewal bioassay test over a 96 h period using a lethal concentration (LC50) value of 0.361mg/mL. Potassium cyanide was highly toxic to the animal tested. Results reveal that normal respiratory activity (O2 consumption) of the fish was significantly affected and there was significant decreased in the Ca2+ - ATPase activities at the end of exposure periods (24, 48, 72 and 96 h). Correlation analysis reveals a strong relationship between oxygen consumption index and ATPase enzyme activity of Clariasgariepinus exposed to the toxicant. This study reflects the toxic effect of potassium cyanide to the freshwater fish, Clariasgariepinus and suggestion on the possible application of Ca2+ -ATPase activities and oxygen consumption index as possible biomarkers for early detection of cyanide poisoning in aquatic bodies.

Heat production and oxygen consumption during metabolic recovery of white muscle fibres from the dogfish Scyliorhinus canicula

2000 ◽  
Vol 203 (7) ◽  
pp. 1201-1210 ◽  
Author(s):  
F. Lou ◽  
W. J. van Der Laarse ◽  
N.A. Curtin ◽  
R.C. Woledge
Keyword(s):  
Oxygen Consumption ◽  
Heat Production ◽  
White Muscle ◽  
Recovery Period ◽  
Atp Synthesis ◽  
Muscle Fibres ◽  
Scyliorhinus Canicula ◽  
Maximum Value ◽  
The Difference ◽  
Time Courses

Oxygen consumption and heat production were measured during contraction and recovery of isolated, white muscle fibres from dogfish (Scyliorhinus canicula) at 19 degrees C. The contraction period consisted of 20 isometric twitches at 3 Hz; this was followed by a recovery period of 2 h without stimulation. We tested the hypothesis that recovery is wholly oxidative (not glycolytic) in these fibres. The following features support this hypothesis. (i) The ratio of total heat produced to oxygen consumed, 451+/−34 kJ mol(−)(1) (mean +/− s.e.m., N=29), was close to that expected for either the oxidation of carbohydrate, 473 kJ mol(−)(1), or the oxidation of fat, 439 kJ mol(−)(1). Even assuming the maximum value (95 % confidence limit) of the observed heat production, glycolysis could account for resynthesis of at most 18 % of the ATP used during the contractions. (ii) When the difference in rates of diffusion of oxygen and heat within the muscle are taken into account, the time courses of oxygen consumption and heat production match each other well during the entire recovery period. The efficiency of recovery (=energy used for ATP synthesis/energy available for ATP synthesis) was estimated from the results. This value, 84.0+/−20.1 % (mean +/− s.e.m., N=29), is relatively high and represents the first such measurement in functioning muscle.

The Function and Metabolism of Certain Insect Muscles in Relation to their Structure

1955 ◽  
Vol s3-96 (34) ◽  
pp. 151-159
Author(s):  
GEORGE A. EDWARDS ◽  
HELMUT RUSKA
Keyword(s):  
Endoplasmic Reticulum ◽  
Oxygen Consumption ◽  
Dehydrogenase Activity ◽  
White Muscle ◽  
Muscle Fibres ◽  
Electron Microscopic ◽  
Leg Muscles ◽  
Red Muscle ◽  
Ultrathin Sections

Electron microscopic observations on ultrathin sections of the red thoracic flightmuscles and white leg muscles of Hydrophilus and Dytiscus are reported. In red muscle-fibres with high values in frequency of contraction, oxygen consumption, and dehydrogenase activity, the single fibrils are completely surrounded by huge mitochondria. Tracheoles penetrate the sarcolemma and supply the mitochondria with oxygen by intracellular branches. In the less active white muscle fibres, mitochondria are found irregularly scattered between the fibrils or along the I band. The intracellular tracheolization is sparse but an endoplasmic reticulum is widely spread between the synfibrillar contractile material. The same muscles of the two insects differ considerably in detail.

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