A Preliminary Study Aimed at the Detection ofLeishmaniaParasites in Subjects with Cutaneous Leishmaniasis Using Polymerase Chain Reaction

1998 ◽  
Vol 25 (5) ◽  
pp. 290-298 ◽  
Author(s):  
Hiroshi Uezato ◽  
Keisuke Hagiwara ◽  
Atsushi Hosokawa ◽  
Motoyoshi Maruno ◽  
Shigeo Nonaka ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cutaneous Leishmaniasis ◽  
Chain Reaction ◽  
Preliminary Study ◽  
Polymerase Chain

scholarly journals Assessment of Aggregatibacter actinomycetemcomitans and Prevotella intermedia Counts around Healthy Implants, Diseased Implants and Sound Teeth: A Preliminary Study

2017 ◽  
Vol 9 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Siamak Yaghobee ◽  
Afshin Khorsand ◽  
Nojan Jahedmanesh ◽  
Mahdi Kadkhodazadeh
Keyword(s):  
Polymerase Chain Reaction ◽  
Gingival Crevicular Fluid ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Prevotella Intermedia ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Standard Curve ◽  
Preliminary Study ◽  
Polymerase Chain ◽  
Crevicular Fluid

Background. This study aimed to assess Aggregatibacter actinomycetemcomitans (Aa) and Prevotella intermedia (PI) counts in gingival crevicular fluid (GCF) around healthy implants, diseased implants and sound teeth. Methods. Eight patients (four males and four females), who had healthy implants, implants with peri-implantitis and sound teeth, were selected. Samples (GCF) were analyzed using real-time polymerase chain reaction (RT-PCR). The above-mentioned bacteria were detected and counted. Data analysis in RT-PCR was carried out based on the standard curve using Prism software to compare Pi and Aa counts between the three areas (GCF around sound teeth, healthy implants and implants with peri-implantitis). Results. Pi counts were significantly higher in GCF around implants with peri-implantitis (8 implants) than around healthy implants (8 implants) (P<0.001) and sound teeth (8) (P=0.012). No significant differences were found in Pi counts in GCF around healthy implants and sound teeth (P=0.063). Aa counts in GCF around implants with peri-implantitis were significantly higher than those around healthy implants (P=0.002) and sound teeth (P=0.024). No significant differences were noted in Aa counts in GCF around healthy implants and sound teeth (P=0.57). Conclusion. Aa and Pi counts in GCF around diseased implants were higher than around healthy implants and sound teeth. Also, Aa counts were significantly higher than Pi counts.

Leishmaniasis

2020 ◽  
pp. 1467-1475
Author(s):  
Antony D.M. Bryceson ◽  
Diana N.J. Lockwood
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Cutaneous Leishmaniasis ◽  
Military Personnel ◽  
Chain Reaction ◽  
Biopsy Material ◽  
Phlebotomine Sandflies ◽  
Polymerase Chain ◽  
Reservoir Hosts ◽  
Animal Reservoirs

Leishmaniasis is caused by parasites of the genus Leishmania, which are transmitted to humans from human or animal reservoirs by the bites of phlebotomine sandflies. In places the disease is common and important, with perhaps 500,000 cases of visceral leishmaniasis and 1.5–2 million cases of cutaneous leishmaniasis worldwide each year. Diagnosis is by demonstration of leishmania organisms in tissue smears or biopsy material by microscopy, culture, or detecting leishmaniai DNA by polymerase chain reaction. As an imported disease, cutaneous leishmaniasis is common in travellers, military personnel, and immigrants coming from endemic areas, while the diagnosis of the less common visceral leishmaniasis is frequently overlooked. Prevention is by controlling reservoir hosts and sandfly vectors, or by avoiding bites by vectors. There is no vaccine.

scholarly journals Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

2020 ◽  
Vol 17 (15) ◽  
pp. 5508 ◽  
Author(s):  
Hana Mlejnkova ◽  
Katerina Sovova ◽  
Petra Vasickova ◽  
Vera Ocenaskova ◽  
Lucie Jasikova ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Wastewater Treatment ◽  
Czech Republic ◽  
Wastewater Treatment Plants ◽  
Chain Reaction ◽  
The Czech Republic ◽  
Untreated Wastewater ◽  
Preliminary Study ◽  
Polymerase Chain

The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.

scholarly journals Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

2019 ◽  
Vol 79 ◽  
pp. 1-3 ◽  
Author(s):  
Juan David Ramírez ◽  
Giovanny Herrera ◽  
Carlos Muskus ◽  
Claudia Mendez ◽  
María Clara Duque ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cutaneous Leishmaniasis ◽  
Chain Reaction ◽  
Polymerase Chain

Immunohistochemistry and polymerase chain reaction on paraffin-embedded material improve the diagnosis of cutaneous leishmaniasis in the Amazon region

2009 ◽  
Vol 48 (10) ◽  
pp. 1091-1095 ◽  
Author(s):  
Valdir Sabbaga Amato ◽  
Felipe Francisco Tuon ◽  
Heitor Franco de Andrade, Jr ◽  
Helio Bacha ◽  
Carla Pagliari ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cutaneous Leishmaniasis ◽  
Amazon Region ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis

2006 ◽  
Vol 54 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Marcos J. Marques ◽  
Ângela C. Volpini ◽  
George L.L. Machado-Coelho ◽  
Jackson Machado-Pinto ◽  
Carlos A. da Costa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cutaneous Leishmaniasis ◽  
Chain Reaction ◽  
Laboratory Methods ◽  
American Cutaneous Leishmaniasis ◽  
Polymerase Chain

scholarly journals Cutaneous Leishmaniasis of Lip and Role of Polymerase Chain Reaction: A Case Report

2020 ◽  
Vol 58 (227) ◽  
Author(s):  
Niraj Parajuli ◽  
Srijan Shrestha ◽  
Krishna Das Manandhar ◽  
Anup Bastola
Keyword(s):  
Polymerase Chain Reaction ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Donovani ◽  
Polymerase Chain Reaction Test ◽  
Molecular Testing ◽  
Chain Reaction ◽  
Chain Reactions ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Chain Reaction Test

The diagnosis of cutaneous leishmaniasis is mostly confirmed by the identification of parasitein a skin smear or biopsy. However, this method may not always be sensitive enough to detectthe disease when parasitic load is low. Molecular test such as polymerase chain reactions canbe useful in such circumstances. Here, we report a case of cutaneous leishmaniasis diagnosedby a polymerase chain reaction test when both smear and biopsy failed to confirm the diagnosis.A 17-years-old female from mountainous district of Nepal, presented with a crusted plaqueover the upper lip for a duration of 6 months. Both skin smear and biopsy from the lesionfailed to demonstrate Leishmania parasite but a polymerase chain reaction test was positivefor Leishmania donovani. This case emphasizes on the importance of molecular testing suchas polymerase chain reaction when commonly performed diagnostics test fails to supportconfirmation of clinical diagnosis.

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