Immunohistochemistry and polymerase chain reaction on paraffin-embedded material improve the diagnosis of cutaneous leishmaniasis in the Amazon region

Leishmaniasis is caused by parasites of the genus Leishmania, which are transmitted to humans from human or animal reservoirs by the bites of phlebotomine sandflies. In places the disease is common and important, with perhaps 500,000 cases of visceral leishmaniasis and 1.5–2 million cases of cutaneous leishmaniasis worldwide each year. Diagnosis is by demonstration of leishmania organisms in tissue smears or biopsy material by microscopy, culture, or detecting leishmaniai DNA by polymerase chain reaction. As an imported disease, cutaneous leishmaniasis is common in travellers, military personnel, and immigrants coming from endemic areas, while the diagnosis of the less common visceral leishmaniasis is frequently overlooked. Prevention is by controlling reservoir hosts and sandfly vectors, or by avoiding bites by vectors. There is no vaccine.

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A Preliminary Study Aimed at the Detection ofLeishmaniaParasites in Subjects with Cutaneous Leishmaniasis Using Polymerase Chain Reaction

The Journal of Dermatology ◽

10.1111/j.1346-8138.1998.tb02400.x ◽

1998 ◽

Vol 25 (5) ◽

pp. 290-298 ◽

Cited By ~ 7

Author(s):

Hiroshi Uezato ◽

Keisuke Hagiwara ◽

Atsushi Hosokawa ◽

Motoyoshi Maruno ◽

Shigeo Nonaka ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Cutaneous Leishmaniasis ◽

Chain Reaction ◽

Preliminary Study ◽

Polymerase Chain

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Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2018.10.029 ◽

2019 ◽

Vol 79 ◽

pp. 1-3 ◽

Cited By ~ 3

Author(s):

Juan David Ramírez ◽

Giovanny Herrera ◽

Carlos Muskus ◽

Claudia Mendez ◽

María Clara Duque ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Cutaneous Leishmaniasis ◽

Chain Reaction ◽

Polymerase Chain

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Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2005.08.003 ◽

2006 ◽

Vol 54 (1) ◽

pp. 37-43 ◽

Cited By ~ 19

Author(s):

Marcos J. Marques ◽

Ângela C. Volpini ◽

George L.L. Machado-Coelho ◽

Jackson Machado-Pinto ◽

Carlos A. da Costa ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Cutaneous Leishmaniasis ◽

Chain Reaction ◽

Laboratory Methods ◽

American Cutaneous Leishmaniasis ◽

Polymerase Chain

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Genotyping of Cutaneous Leishmaniasis Cases Detected Before and After Migration with Real-Time Polymerase Chain Reaction in Hatay

Turkish Journal of Parasitology ◽

10.4274/tpd.galenos.2020.6650 ◽

2020 ◽

Vol 44 (1) ◽

pp. 48-51

Author(s):

Gülnaz Çulha ◽

Tuğba Kaya ◽

Asena Çiğdem Doğramacı

Keyword(s):

Polymerase Chain Reaction ◽

Cutaneous Leishmaniasis ◽

Real Time ◽

Chain Reaction ◽

Before And After ◽

Polymerase Chain

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Cutaneous Leishmaniasis of Lip and Role of Polymerase Chain Reaction: A Case Report

Journal of Nepal Medical Association ◽

10.31729/jnma.5025 ◽

2020 ◽

Vol 58 (227) ◽

Author(s):

Niraj Parajuli ◽

Srijan Shrestha ◽

Krishna Das Manandhar ◽

Anup Bastola

Keyword(s):

Polymerase Chain Reaction ◽

Cutaneous Leishmaniasis ◽

Leishmania Donovani ◽

Polymerase Chain Reaction Test ◽

Molecular Testing ◽

Chain Reaction ◽

Chain Reactions ◽

Reaction Test ◽

Polymerase Chain ◽

Chain Reaction Test

The diagnosis of cutaneous leishmaniasis is mostly confirmed by the identification of parasitein a skin smear or biopsy. However, this method may not always be sensitive enough to detectthe disease when parasitic load is low. Molecular test such as polymerase chain reactions canbe useful in such circumstances. Here, we report a case of cutaneous leishmaniasis diagnosedby a polymerase chain reaction test when both smear and biopsy failed to confirm the diagnosis.A 17-years-old female from mountainous district of Nepal, presented with a crusted plaqueover the upper lip for a duration of 6 months. Both skin smear and biopsy from the lesionfailed to demonstrate Leishmania parasite but a polymerase chain reaction test was positivefor Leishmania donovani. This case emphasizes on the importance of molecular testing suchas polymerase chain reaction when commonly performed diagnostics test fails to supportconfirmation of clinical diagnosis.

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