Microsatellite (CA)n polymorphism in intron 2 of the canine rod photoreceptor cGMP-phosphodiesterase α-subunit gene

1997 ◽  
Vol 28 (5) ◽  
pp. 380-380 ◽  
Author(s):  
A. Veske ◽  
S E G Nilsson ◽  
A. Gal
Keyword(s):  
Rod Photoreceptor ◽  
Subunit Gene ◽  
Α Subunit ◽  
Cgmp Phosphodiesterase ◽  
Intron 2

Molecular characterization of human and bovine rod photoreceptor cGMP phosphodiesterase α-subunit and chromosomal localization of the human gene

Genomics ◽  
1990 ◽  
Vol 6 (2) ◽  
pp. 272-283 ◽  
Author(s):  
Steven J. Pittler ◽  
Wolfgang Baehr ◽  
John J. Wasmuth ◽  
David G. McConnell ◽  
Mary S. Champagne ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Chromosomal Localization ◽  
Human Gene ◽  
Rod Photoreceptor ◽  
Α Subunit ◽  
Cgmp Phosphodiesterase

scholarly journals Functional Analysis of the Rod Photoreceptor cGMP Phosphodiesterase α-Subunit Gene Promoter

2004 ◽  
Vol 279 (19) ◽  
pp. 19800-19807 ◽  
Author(s):  
Steven J. Pittler ◽  
Youwen Zhang ◽  
Shiming Chen ◽  
Alan J. Mears ◽  
Donald J. Zack ◽  
...  
Keyword(s):  
Binding Sites ◽  
Promoter Region ◽  
Promoter Activity ◽  
Core Promoter ◽  
Dnase I ◽  
Hek293 Cells ◽  
Upstream Sequence ◽  
Rod Photoreceptor ◽  
Α Subunit ◽  
Cgmp Phosphodiesterase

To understand the factors controlling expression of the cGMP phosphodiesterase type 6 (PDE6) genes, we have characterized the promoter of the human PDE6A gene that encodes the catalytic α-subunit.In vivoDNase I hypersensitivity assays revealed two sites immediately upstream of the PDE6A core promoter region. Transient transfection assay in Y79 cells of constructs containing varying lengths of the promoter region showed a decrease in promoter activity with increasing length. The most active segment contained a 177-bp upstream sequence including apparent Crx and Nrl transcription factor binding sites. Both Crx and Nrl transactivated the PDE6A promoter in HEK293 cells and showed a >100-fold increase when coexpressed. Coexpression of a dominant negative inhibitor of Nrl abolished Nrl transactivation but had no effect on Crx. DNase I footprinting assays identified three potential Crx binding sites within a 55-bp segment beginning 29 bp upstream of the transcription start point. Mutation of two of these sites reduced reporter gene activity by as much as 69%. Gel shifts showed that all three Crx sites required a TAAT sequence for efficient binding. Consistent with a requirement for Crx and Nrl inPde6apromoter activity,Pde6amRNA is reduced by 87% in the retina of Crx–/–mice and is undetectable in Nrl–/–mice at postnatal day 10. These results establish that both Nrl and Crx are required for full transcriptional activity of the PDE6A gene.

Structure and upstream region characterization of the human gene encoding rod photoreceptor cGMP phosphodiesterase α-subunit

1998 ◽  
Vol 10 (3) ◽  
pp. 235-250 ◽  
Author(s):  
Mohamed K. Mohamed ◽  
Russell E. Taylor ◽  
Douglas S. Feinstein ◽  
Xizhong Huang ◽  
Steven J. Pittler
Keyword(s):  
Human Gene ◽  
Upstream Region ◽  
Rod Photoreceptor ◽  
Gene Encoding ◽  
Α Subunit ◽  
Cgmp Phosphodiesterase

Chromosome mapping of the rod photoreceptor cGMP phosphodiesterase β-subunit gene in mouse and human: Tight linkage to the Huntington disease region (4p16.3)

Genomics ◽  
1992 ◽  
Vol 12 (4) ◽  
pp. 750-754 ◽  
Author(s):  
Michael R. Altherr ◽  
John J. Wasmuth ◽  
Michael F. Seldin ◽  
Joseph H. Nadeau ◽  
Wolfgang Baehr ◽  
...  
Keyword(s):  
Huntington Disease ◽  
Chromosome Mapping ◽  
Rod Photoreceptor ◽  
Β Subunit ◽  
Subunit Gene ◽  
Cgmp Phosphodiesterase ◽  
Tight Linkage ◽  
Disease Region

Expression of inhibin α-subunit gene during mouse gametogenesis

1990 ◽  
Vol 44 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Shigenobu Tone ◽  
Yuko Katoh ◽  
Hirokazu Fujimoto ◽  
Shin Togashi ◽  
Masako Yanazawa ◽  
...  
Keyword(s):  
Subunit Gene ◽  
Α Subunit

scholarly journals Nrl and Sp Nuclear Proteins Mediate Transcription of Rod-specific cGMP-phosphodiesterase β-Subunit Gene

2001 ◽  
Vol 276 (37) ◽  
pp. 34999-35007 ◽  
Author(s):  
Leonid E. Lerner ◽  
Yekaterina E. Gribanova ◽  
Ming Ji ◽  
Barry E. Knox ◽  
Debora B. Farber
Keyword(s):  
Nuclear Proteins ◽  
Β Subunit ◽  
Subunit Gene ◽  
Cgmp Phosphodiesterase
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