Genetic variation in eggshell crystal size and orientation is large and these traits are correlated with shell thickness and are associated with eggshell matrix protein markers

For the first time, genetic diversity among 14 ornamental palm accessions originating from different countries and grown in different regions in Egypt were examined. Identification of genetic variation and phylogenetic relationships in ornamental palms would be useful for its genetic identification, improvement, and conservation. Genetic polymorphism was analyzed using the randomly amplified polymorphic DNA (RAPD) as well as protein markers. The electrophoretic pattern of protein analysis produced 21 bands distributed in all accessions with molecular sizes ranging from 11.8 to 99.3 KDa. Some accessions possessed some bands, which were absent in other accessions and could be used for their identification. Furthermore, 10 RAPD selected primers were employed to determine genetic variation among the 14 palm genotypes as well as to test the effectiveness of RAPD primers as a genetic marker. RAPD analysis revealed a high level of polymorphism (100%) among the studied accessions. A total number of 310 amplified bands were generated across the studied genotypes with an average of 30 bands per primer. Cluster analysis using sequence alignment was done to generate a dendrogram verifying the relationship among the 14 studied ornamental palms, with an average similarity matrix range of 0.00 to 0.08 and 0.39 to 0.93 for RAPD and protein markers, respectively. It is concluded that, both SDS-protein and RAPD markers are equally important for genetic analysis and are suitable for the characterization of ornamental palm collection.

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Purification of Ovocalyxin-32, a Novel Chicken Eggshell Matrix Protein

Connective Tissue Research ◽

10.1080/713713647 ◽

2003 ◽

Vol 44 (1) ◽

pp. 16-19 ◽

Cited By ~ 1

Author(s):

M. T. Hincke ◽

J. Gautron ◽

K. Mann ◽

M. Panhéleux ◽

M. D. McKee ◽

...

Keyword(s):

Matrix Protein ◽

Chicken Eggshell ◽

Eggshell Matrix

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Purification, Characterization, andin VitroMineralization Studies of a Novel Goose Eggshell Matrix Protein, Ansocalcin

Journal of Biological Chemistry ◽

10.1074/jbc.m201518200 ◽

2002 ◽

Vol 278 (5) ◽

pp. 2928-2936 ◽

Cited By ~ 43

Author(s):

Rajamani Lakshminarayanan ◽

Suresh Valiyaveettil ◽

Veena S. Rao ◽

R. Manjunatha Kini

Keyword(s):

Matrix Protein ◽

Eggshell Matrix

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Ovocalyxin-32, a Novel Chicken Eggshell Matrix Protein

Journal of Biological Chemistry ◽

10.1074/jbc.m104543200 ◽

2001 ◽

Vol 276 (42) ◽

pp. 39243-39252 ◽

Cited By ~ 99

Author(s):

Joël Gautron ◽

Maxwell T. Hincke ◽

Karlheinz Mann ◽

Marina Panhéleux ◽

Maureen Bain ◽

...

Keyword(s):

Matrix Protein ◽

Chicken Eggshell ◽

Eggshell Matrix

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GENETIC VARIATION IN INDIGENOUS TURKEY POPULATIONS IN SOUTH WEST NIGERIA

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v7i2.5967 ◽

2017 ◽

Vol 7 (2) ◽

pp. 1021-1029

Author(s):

Fatai R. B ◽

Akinyemi M.O ◽

Osaiyuwu O. H

Keyword(s):

Genetic Variation ◽

Genetic Distance ◽

Conservation Strategies ◽

Fixation Index ◽

Genetic Integrity ◽

Red Cell ◽

Protein Markers ◽

Cellulose Acetate Electrophoresis ◽

South West ◽

Hardy Weinberg Equilibrium

The study was carried out to assess the genetic variation in indigenous turkey populations in South West Nigeria using seven protein markers: Hemoglobin (Hb), Albumin (Alb), Transferin (Tf), Esterase-1 (Es-1), Alkaline phosphatase (ALP), Carbonic anhydrase (CA), and Esterase-3 (Es-3).. A total of 97 turkey birds from Ibadan, Ijebu Ode and Akure were purposively sampled. Separation of plasma and red cell proteins were determined on cellulose acetate electrophoresis to estimate the genetic variability within and among the populations. The degree of heterozygosity, deviation from Hardy-Weinbergs Equilibrum (HWE), FIS, and FST values were estimated. From a total of 7 loci analyzed, six were found to be polymorphic in all the populations sampled while Es-1 was monomorphic. Two codominant alleles which controlled three different genotypes were observed at each polymorphic loci. Deviations from Hardy Weinberg equilibrium and a deficiency of heterozygotes were observed in all the populations. Average heterozygosities ranged between 0.13 and 0.20, heterozygote deficit FIT was estimated at 0.5784 while within breed deficit as evaluated by FIS ranged from 0.38 to 0.56. The fixation index FST revealed that genetic diversity within the studied population was moderately differentiated. Genetic distance among the populations quantified through calculation of Nei genetic distance ranged from 0.037 - 0.109. The result obtained may be used as an initial guide in defining objectives for future investigations of genetic integrity and developing conservation strategies for Nigeria indigenous turkeys.

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Genetic characterization of genotypes of Austrian pine (Pinus nigra Arnold) populations using protein markers

Genetika ◽

10.2298/gensr0802157l ◽

2008 ◽

Vol 40 (2) ◽

pp. 157-168 ◽

Cited By ~ 3

Author(s):

Aleksandar Lucic ◽

Ana Nikolic ◽

Snezana Mladenovic-Drinic ◽

Vasilije Isajev ◽

Vera Lavadinovic

Keyword(s):

Genetic Variation ◽

Protein Profile ◽

Seed Proteins ◽

Population Level ◽

Pinus Nigra ◽

Population Based ◽

Protein Fractions ◽

Protein Markers ◽

Erosion Processes ◽

Population Genetic Variation

The occurrence of Austrian pine over large areas attacked by erosion processes or on completely degraded and denuded areas on which it produces excellent results, classifies it among the most important economic species in forestry. The aim of present research is to study intra- and inter-population genetic variation in Austrian pine by the application of biochemical markers. A better knowledge of the genetic potential of Austrian pine populations will enhance the production of seed and planting material, and in this way also the success of a forestation and the establishment of Austrian pine specific-purpose plantations. The polymorphism of protein markers was determined based on the selected genotypes originating from six populations (seed stands), which actually included all Austrian pine populations in Serbia. Based on the derived electrophoregram, qualitative and quantitative differences (number and pattern) in protein fractions were identified and the seed protein profile was constructed for each tree, as well as for each of the six study populations. This showed the 'conservativeness' of some polypeptides, i.e. the presence in all analyzed genotypes. On the other hand, some protein fractions were variable at the population level, and some were variable depending on the population. Based on the electrophoregram, the coefficient of genetic similarity/distance was calculated by comparing the band numbers and patterns. Cluster analysis dendrograms were also constructed. The degree of genetic variation among the provenances was higher than the variation within the provenances. There was also a high genetic variation in seed proteins within the analyzed populations.

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