Use of microsatellite loci and AFLP markers to verify gynogenesis and clonal lines in Nile tilapia Oreochromis niloticus L.

Polymorphisms in growth hormone genes have been found to cause variation in growth performance of fish. The objective of the study was to reveal variations in microsatellite loci located in the growth hormone genes of Nile tilapia (Oreochromis niloticus). Five microsatellite loci namely GH-MS01, IGFII, IGFII-MS01, IGFII-MS03, and STR were analyzed to assess the genetic variation in the growth hormone genes of four stocks of O. niloticus viz. FBG-Mini Hatchery, FM-Mini hatchery, Eon Aquaculture Ltd. and BFRI. The microsatellite markers were amplified by polymerase chain reaction, separated by polyacrylamide gel electrophoresis and visualized through ethidium bromide staining. All the five loci were found to be polymorphic. The average number of alleles of FM-Mini hatchery stock (3.8) was found to be highest and that of the FBG-Mini hatchery (2.8) and Eon Aquaculture stocks was found to be lowest. The average observed heterozygosity (Ho) value of the FM-Mini hatchery stock was the highest (0.140) and that of FBG-Mini hatchery stock was the lowest (0.040). On the other hand, the average expected heterozygosity (He) was highest in the BFRI stock (0.660) and lowest in the FM-Mini hatchery and FBG-Mini hatchery stock (0.432). The fixation index (1 - (Ho / He) values were positive in all the loci (except locus GH-MS01 in Eon Aquaculture stock), which means these stocks (O. niloticus) were deficient in heterozygosity. Deviation from Hardy-Weinberg expectation at STR locus in FBG-Mini hatchery and Eon Aquaculture stocks were not significant but in all other cases the deviations were found to be significant. The results provide evidence that genetic variation exists within the growth hormone genes in all four stocks of O. niloticus. The polymorphisms that have been detected in the present study can be used to study association with growth and thus selection of fast growing Nile tilapia in Bangladesh.

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Characterization of Francisella noatunensis subsp. orientalis isolated from Nile tilapia Oreochromis niloticus farmed in Lake Yojoa, Honduras

Diseases of Aquatic Organisms ◽

10.3354/dao03333 ◽

2019 ◽

Vol 133 (2) ◽

pp. 141-145 ◽

Cited By ~ 2

Author(s):

E Soto ◽

K Shahin ◽

JJ Talhami ◽

MJ Griffin ◽

A Adams ◽

...

Keyword(s):

Oreochromis Niloticus ◽

Nile Tilapia ◽

Nile Tilapia Oreochromis Niloticus

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The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2014.7302 ◽

2014 ◽

pp. 27

Author(s):

Arne A Ratulangi ◽

Reiny Tumbol ◽

Hengky Manoppo ◽

Henneke Pangkey

Keyword(s):

Survival Rate ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Bacterial Disease ◽

Immersion Method ◽

Nile Tilapia Oreochromis Niloticus ◽

Different Ages ◽

Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

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