scholarly journals Microsatellite marker-based variation in the growth hormone genes of Nile tilapia (Oreochromis niloticus)

2020 ◽  
Vol 32 (1) ◽  
pp. 1-10
Author(s):  
Mst. Sadia Zafrin ◽  
Md Samsul Alam
Keyword(s):  
Growth Hormone ◽  
Genetic Variation ◽  
Microsatellite Loci ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Polyacrylamide Gel Electrophoresis ◽  
Fixation Index ◽  
Hatchery Stock ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Ethidium Bromide Staining

Polymorphisms in growth hormone genes have been found to cause variation in growth performance of fish. The objective of the study was to reveal variations in microsatellite loci located in the growth hormone genes of Nile tilapia (Oreochromis niloticus). Five microsatellite loci namely GH-MS01, IGFII, IGFII-MS01, IGFII-MS03, and STR were analyzed to assess the genetic variation in the growth hormone genes of four stocks of O. niloticus viz. FBG-Mini Hatchery, FM-Mini hatchery, Eon Aquaculture Ltd. and BFRI. The microsatellite markers were amplified by polymerase chain reaction, separated by polyacrylamide gel electrophoresis and visualized through ethidium bromide staining. All the five loci were found to be polymorphic. The average number of alleles of FM-Mini hatchery stock (3.8) was found to be highest and that of the FBG-Mini hatchery (2.8) and Eon Aquaculture stocks was found to be lowest. The average observed heterozygosity (Ho) value of the FM-Mini hatchery stock was the highest (0.140) and that of FBG-Mini hatchery stock was the lowest (0.040). On the other hand, the average expected heterozygosity (He) was highest in the BFRI stock (0.660) and lowest in the FM-Mini hatchery and FBG-Mini hatchery stock (0.432). The fixation index (1 - (Ho / He) values were positive in all the loci (except locus GH-MS01 in Eon Aquaculture stock), which means these stocks (O. niloticus) were deficient in heterozygosity. Deviation from Hardy-Weinberg expectation at STR locus in FBG-Mini hatchery and Eon Aquaculture stocks were not significant but in all other cases the deviations were found to be significant. The results provide evidence that genetic variation exists within the growth hormone genes in all four stocks of O. niloticus. The polymorphisms that have been detected in the present study can be used to study association with growth and thus selection of fast growing Nile tilapia in Bangladesh.  

Transgenic Nile tilapia (Oreochromis niloticus) over-expressing growth hormone show reduced ammonia excretion

Aquaculture ◽  
2007 ◽  
Vol 270 (1-4) ◽  
pp. 427-435 ◽  
Author(s):  
Snin-ichiro Kobayashi ◽  
Alimuddin ◽  
Tetsuro Morita ◽  
Misako Miwa ◽  
Jun Lu ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Ammonia Excretion ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Exclusion of two microsatellite markers in the sex-linkage study of Nile tilapia (Oreochromis niloticus L.)

2019 ◽  
Vol 6 (1) ◽  
pp. 143-151
Author(s):  
Marufa Sultana Mitu ◽  
Antima Gani ◽  
Md Bakhtiar Abid ◽  
Sadia Nusrat Sharna ◽  
Farzana Yesmin ◽  
...  
Keyword(s):  
Sex Determination ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sex Linkage ◽  
Female Progeny ◽  
Male Progeny ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Determination System ◽  
Sex Determination System

Monosex Nile tilapia (Oreochromis niloticus) is highly preferred in semi-intensive and intensive culture systems to prevent uncontrolled reproduction and to obtain fast growing male. Production of all male tilapia is being practiced by the hatcheries of Bangladesh mainly by administering androgen hormones (particularly 17-α-methyl-testosterone) with feed in a mixture of undifferentiated fry for about a month. The direct application of hormone to such food chain often arises question in respect to public health and safety. The alternative to this is the production of putative supermales, a rather safe but longer procedure to obtain all male progeny. However, sex determination system in tilapia is fairly complex. Recent developments have resulted in a linkage map and genetic markers that can be used to analyze the sex determination system. For genetic analysis of different genotypes of fish, microsatellite DNA marker ARO120 and ARO121 were used for studying the inheritance pattern for possible sex linkage using Polyacrylamide gel electrophoresis. In case of ARO120, it was observed that the Dam XX was heterozygous; 11 out of 22 female progeny and 10 out of 22 male progeny were found to be heterozygous. In case of ARO121, it was observed that the Dam XX was heterozygous; 16 out of 22 female progeny and 20 out of 22 male progeny were found to be heterozygous. Though the marker polymorphisms were observed in this study, these were excluded from the sexlinkage study due to limited extent of information as sex-linked markers in Nile tilapia BFRI strain. This study provides a baseline for further research using other suitable polymorphic markers for assisting marker-assisted selection. Res. Agric., Livest. Fish.6(1): 143-151, April 2019

Genetic improvement of farmed tilapias: estimation of genetic variation and heritability for age and size at first spawning in Nile tilapia (Oreochromis niloticus)

Aquaculture ◽  
1995 ◽  
Vol 137 (1-4) ◽  
pp. 279-280 ◽  
Author(s):  
Ambekar E. Eknath ◽  
Ruben A. Reyes ◽  
Hernando L. Bolivar ◽  
Marietta P. De Vera ◽  
Jodecel C. Danting ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Oreochromis Niloticus ◽  
Genetic Improvement ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus
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