Effect of vitamin A on the skeletal morphogenesis of European sea bass, Dicentrarchus labrax (Linnaeus, 1758)

The effect of the feeding period on larval development was investigated in European sea bass larvae by considering the expression level of some genes involved in morphogenesis. Larvae were fed a control diet except during three different periods (period A: from 8 to 13d post-hatching (dph); period B: from 13 to 18dph; period C: from 18 to 23dph) with two compound diets containing high levels of vitamin A or PUFA. European sea bass morphogenesis was affected by these two dietary nutrients during the early stages of development. The genes involved in morphogenesis could be modulated between 8 and 13dph, and our results indicated that retinoids and fatty acids influenced two different molecular pathways that in turn implicated two different gene cascades, resulting in two different kinds of malformation. Hypervitaminosis A delayed development, reducing the number of vertebral segments and disturbing bone formation in the cephalic region. These malformations were correlated to an upregulation of retinoic acid receptor γ, retinoid X receptor (RXR) α and bone morphogenetic protein (BMP)4. An excess of PUFA accelerated the osteoblast differentiation process through the upregulation of RXRα and BMP4, leading to a supernumerary vertebra. These results suggest that the composition of diets devoted to marine fish larvae has a particularly determining effect before 13dph on the subsequent development of larvae and juvenile fish.

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Vaccination of European sea bass Dicentrarchus labrax with avirulent Mycobacterium marinum (iipA::kan mutant)

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2019.04.057 ◽

2019 ◽

Vol 90 ◽

pp. 317-327 ◽

Cited By ~ 1

Author(s):

Shay Ravid-Peretz ◽

Angelo Colorni ◽

Galit Sharon ◽

Michal Ucko

Keyword(s):

Dicentrarchus Labrax ◽

Sea Bass ◽

Mycobacterium Marinum ◽

European Sea Bass

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Cortisol concentration in scales is a valid indicator for the assessment of chronic stress in European sea bass, Dicentrarchus labrax L

Aquaculture ◽

10.1016/j.aquaculture.2021.737257 ◽

2021 ◽

pp. 737257

Author(s):

A. Samaras ◽

A. Dimitroglou ◽

S. Kollias ◽

G. Skouradakis ◽

I.E. Papadakis ◽

...

Keyword(s):

Chronic Stress ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Cortisol Concentration ◽

European Sea Bass

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Cellular alterations in different organs of European sea bass Dicentrarchus labrax (L.) exposed to cadmium

Chemosphere ◽

10.1016/j.chemosphere.2006.10.061 ◽

2007 ◽

Vol 67 (6) ◽

pp. 1171-1181 ◽

Cited By ~ 83

Author(s):

L. Giari ◽

M. Manera ◽

E. Simoni ◽

B.S. Dezfuli

Keyword(s):

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass ◽

Cellular Alterations

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Developmental expression of three different prepro-GnRH (Gonadotrophin-releasing hormone) messengers in the brain of the European sea bass (Dicentrarchus labrax)

Journal of Chemical Neuroanatomy ◽

10.1016/s0891-0618(02)00004-2 ◽

2002 ◽

Vol 23 (4) ◽

pp. 255-267 ◽

Cited By ~ 44

Author(s):

David González-Martı́nez ◽

Nilli Zmora ◽

Silvia Zanuy ◽

Carmen Sarasquete ◽

Abigail Elizur ◽

...

Keyword(s):

Dicentrarchus Labrax ◽

Sea Bass ◽

Developmental Expression ◽

European Sea Bass ◽

Releasing Hormone ◽

Gonadotrophin Releasing Hormone ◽

The Brain

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Morphology of the european sea bass (dicentrarchus labrax) tongue

Microscopy Research and Technique ◽

10.1002/jemt.21105 ◽

2011 ◽

Vol 75 (5) ◽

pp. 643-649 ◽

Cited By ~ 15

Author(s):

Francesco Abbate ◽

Maria Cristina Guerrera ◽

Giuseppe Montalbano ◽

Felix De Carlos ◽

Alberto Álvarez Suárez ◽

...

Keyword(s):

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass

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Analysis of the Sox gene family in the European sea bass (Dicentrarchus labrax)

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpc.2003.12.002 ◽

2004 ◽

Vol 137 (2) ◽

pp. 279-284 ◽

Cited By ~ 22

Author(s):

Malyka Galay-Burgos ◽

Lynda Llewellyn ◽

Constantinos C Mylonas ◽

Adelino V.M Canario ◽

Silvia Zanuy ◽

...

Keyword(s):

Gene Family ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass ◽

Sox Gene

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Antioxidant Activities and Selenogene Transcription in the European Sea Bass (Dicentrarchus labrax) Liver Depend, in a Non-linear Manner, on the Se/Hg Molar Ratio of the Feeds

Biological Trace Element Research ◽

10.1007/s12011-021-02835-7 ◽

2021 ◽

Author(s):

Marinelle Espino ◽

Harkaitz Eguiraun ◽

Oihane Diaz de Cerio ◽

José Antonio Carrero ◽

Nestor Etxebarria ◽

...

Keyword(s):

Antioxidant Activities ◽

Dicentrarchus Labrax ◽

Thioredoxin Reductase ◽

Sea Bass ◽

Molar Ratio ◽

Antioxidant Systems ◽

European Sea Bass ◽

Thioredoxin System ◽

Non Linear ◽

Linear Manner

AbstractFeeding 3.9 and 6.7 mg Hg/kg (Se/Hg molar ratios of 0.8 and 0.4, respectively) for 14 days negatively affected Dicentrarchus labrax growth and total DNTB- and thioredoxin-reductase (TrxR) activities and the transcription of four redox genes (txn1, gpx1, txnrd3, and txnrd2) in the liver, but a diet with 0.5 mg Hg/kg (Se/Hg molar ratio 6.6) slightly increased both reductase activities and the transcription of txn1, gpx1, and txnrd2. Feeding 6.7 mg Hg/kg for 53 days downregulated the genes of the thioredoxin system (txn1, txnrd3, and txnrd2) but upregulated gpx1, confirming the previously proposed complementarity among the antioxidant systems. Substitution of 20% of the feed by thawed white fish (hake) slightly counteracted the negative effects of Hg. The effects were not statistically significant and were dependent, in a non-linear manner, on the Se/Hg molar ratio of the feed but not on its Hg concentration. These results stress the need to consider the Se/Hg molar ratio of the feed/food when evaluating the toxicity of Hg.

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