Intake of high levels of vitamin A and polyunsaturated fatty acids during different developmental periods modifies the expression of morphogenesis genes in European sea bass (Dicentrarchus labrax)

The effect of the feeding period on larval development was investigated in European sea bass larvae by considering the expression level of some genes involved in morphogenesis. Larvae were fed a control diet except during three different periods (period A: from 8 to 13d post-hatching (dph); period B: from 13 to 18dph; period C: from 18 to 23dph) with two compound diets containing high levels of vitamin A or PUFA. European sea bass morphogenesis was affected by these two dietary nutrients during the early stages of development. The genes involved in morphogenesis could be modulated between 8 and 13dph, and our results indicated that retinoids and fatty acids influenced two different molecular pathways that in turn implicated two different gene cascades, resulting in two different kinds of malformation. Hypervitaminosis A delayed development, reducing the number of vertebral segments and disturbing bone formation in the cephalic region. These malformations were correlated to an upregulation of retinoic acid receptor γ, retinoid X receptor (RXR) α and bone morphogenetic protein (BMP)4. An excess of PUFA accelerated the osteoblast differentiation process through the upregulation of RXRα and BMP4, leading to a supernumerary vertebra. These results suggest that the composition of diets devoted to marine fish larvae has a particularly determining effect before 13dph on the subsequent development of larvae and juvenile fish.

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Effects of Polyunsaturated Fatty Acids and Prostaglandins on Oocyte Maturation in a Marine Teleost, the European Sea Bass (Dicentrarchus labrax)1

Biology of Reproduction ◽

10.1095/biolreprod64.1.382 ◽

2001 ◽

Vol 64 (1) ◽

pp. 382-389 ◽

Cited By ~ 113

Author(s):

Lisa Ann Sorbera ◽

Juan Francisco Asturiano ◽

Manuel Carrillo ◽

Silvia Zanuy

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Oocyte Maturation ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Marine Teleost ◽

European Sea Bass

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Effect of vitamin A on the skeletal morphogenesis of European sea bass, Dicentrarchus labrax (Linnaeus, 1758)

Aquaculture Research ◽

10.1111/j.1365-2109.2010.02676.x ◽

2010 ◽

Vol 42 (5) ◽

pp. 684-692 ◽

Cited By ~ 9

Author(s):

Ioanna Georga ◽

Nomiki Glynatsi ◽

Athanasios Baltzois ◽

Dimitrios Karamanos ◽

David Mazurais ◽

...

Keyword(s):

Vitamin A ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass

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Incorporation of dietary fatty acid in European sea bass (Dicentrarchus labrax) — A methodological approach evidencing losses of highly unsaturated fatty acids

Aquaculture ◽

10.1016/j.aquaculture.2006.09.044 ◽

2007 ◽

Vol 263 (1-4) ◽

pp. 227-237 ◽

Cited By ~ 11

Author(s):

J.H. Robin ◽

A. Skalli

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Unsaturated Fatty Acids ◽

Methodological Approach ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Dietary Fatty Acid ◽

European Sea Bass ◽

Highly Unsaturated Fatty Acids

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Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from European sea bass

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.025122-0 ◽

2012 ◽

Vol 62 (2) ◽

pp. 425-429 ◽

Cited By ~ 44

Author(s):

Maximino Piñeiro-Vidal ◽

Daniel Gijón ◽

Carles Zarza ◽

Ysabel Santos

Keyword(s):

Fatty Acids ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Rrna Gene ◽

European Sea Bass ◽

Phenotypic Data ◽

Gene Sequence Analysis ◽

The Family ◽

Total Fatty Acids ◽

Type Strains

A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09T, was isolated from diseased European sea bass (Dicentrarchus labrax L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum (family Flavobacteriaceae, phylum ‘Bacteroidetes’). Sequence similarities between the isolate and type strains of other members of the genus were 93.1–97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C15 : 0 (24.8 %), iso-C15 : 0 3-OH (18.0 %), anteiso-C15 : 0 (8.1 %), C15 : 1ω6c (6.9 %) and iso-C15 : 1 (6.2 %). Genotypic and phenotypic data indicate that strain 35/09T should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum dicentrarchi sp. nov. is proposed; the type strain is 35/09T ( = CECT 7612T = NCIMB 14598T).

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Requirement of n-3 long chain polyunsaturated fatty acids for European sea bass (Dicentrarchus labrax) juveniles: growth and fatty acid composition

Aquaculture ◽

10.1016/j.aquaculture.2004.06.036 ◽

2004 ◽

Vol 240 (1-4) ◽

pp. 399-415 ◽

Cited By ~ 72

Author(s):

Ali Skalli ◽

Jean H. Robin

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Polyunsaturated Fatty Acids ◽

Acid Composition ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass ◽

Long Chain

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Potential application and beneficial effects of a marine microalgal biomass produced in a high-rate algal pond (HRAP) in diets of European sea bass, Dicentrarchus labrax

Environmental Science and Pollution Research ◽

10.1007/s11356-021-14927-x ◽

2021 ◽

Author(s):

Giulia Pascon ◽

Maria Messina ◽

Lisa Petit ◽

Luisa Maria Pinheiro Valente ◽

Beatriz Oliveira ◽

...

Keyword(s):

Control Diet ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Nutrient Digestibility ◽

Fish Growth ◽

High Rate ◽

European Sea Bass ◽

Microalgal Biomass ◽

Distal Intestine ◽

Plant Protein Sources

AbstractMicroalgae have been used as live food in aquatic species. In recent years, the interest in microalgae has considerably increased, thanks to the evolution of production techniques that have identified them as an ecologically attractive aquafeed ingredient. The present study provides the first data about the effects of dietary inclusion of a microalgae consortium grown in a high-rate algal pond system on zootechnical performance, morphometric indices, and dietary nutrient digestibility as well as morphology and functionality of the digestive system of European sea bass, Dicentrarchus labrax. A dietary treatment including a commercial mono-cultured microalgae (Nannochloropsis sp.) biomass was used for comparison. Six hundred and thirty-six European sea bass juveniles (18 ± 0.28 g) were randomly allotted into 12 experimental groups and fed 4 different diets for 10 weeks: a control diet based on fish meal, fish oil, and plant protein sources; a diet including 10% of Nannochloropsis spp. biomass (100 g/kg diet); and two diets including two levels (10% and 20%) of the microalgal consortium (100 and 200 g/kg diet). Even at the highest dietary inclusion level, the microalgal consortium (200 g/kg diet) did not affect feed palatability and fish growth performance. A significant decrease in the apparent digestibility of dry matter, protein, and energy was observed in diets including 10 and 20% of the microalgal consortium, but all fish exhibited a well-preserved intestinal histomorphology. Moreover, dietary inclusion with the microalgal consortium significantly increased the enzymatic activity of maltase, sucrase-isomaltase, and ɤ-glutamil transpeptidase in the distal intestine of the treated European sea bass. Algal consortium grown using fish farm effluents represents an attempt to enhance the utilization of natural biomasses in aquafeeds when used at 10 % as substitute of vegetable ingredients in diet for European sea bass.

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Determination of Hematological Status as Health Indicator of European Sea Bass (Dicentrarchus labrax L.) Fed Different Dietary Fatty Acids

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v5i4.445-450.1106 ◽

2017 ◽

Vol 5 (4) ◽

pp. 445

Author(s):

Aysel Şahan ◽

Hatice Asuman Yılmaz ◽

Orhan Tufan Erdoğan

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fish Oil ◽

Canola Oil ◽

Hematological Parameters ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Cottonseed Oil ◽

Dietary Fatty Acids ◽

European Sea Bass

In this study, the European Sea Bass (Dicentrarchus labrax) were fed rich canola oil which is containing monounsaturated fatty acid (MUFA) and rich cottonseed oil which is containing n-6 polyunsaturated fatty acid (PUFA) and the effects of these feeds on some hematological parameters were investigated. Experimental fish were fed two times daily with 100% fish oil (FO-control), 100% cottonseed oil (CSO), 100% canola oil (CO) and 50% CSO - 50% CO (CSO50-CO50) for 162 days. End of the experiment, total erythrocyte (RBC) amount was highest in the CSO group 269.0 x (104 mm3 -1) compared to all other groups. Leukocyte (WBC) 96.280 x (103 mm3 -1), monocyte, lymphocyte and neutrophil amounts showed significant increases in only CSO group. Therefore, in terms of fish health and welfare, the exclusive use of cottonseed oil without mixing with the other vegetable oils in sea bass diet formulation can be a viable alternative to fish oil since it has the most suitable fatty acids.

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Is the perceived resiliency of fish larvae to ocean acidification masking more subtle effects?

Biogeosciences Discussions ◽

10.5194/bgd-10-17043-2013 ◽

2013 ◽

Vol 10 (10) ◽

pp. 17043-17070 ◽

Cited By ~ 4

Author(s):

E. C. Pope ◽

R. P. Ellis ◽

M. Scolamacchia ◽

J. W. S. Scolding ◽

A. Keay ◽

...

Keyword(s):

Ocean Acidification ◽

Early Life ◽

Fish Larvae ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Life Stages ◽

Daily Mortality ◽

Early Life Stages ◽

European Sea Bass ◽

Near Future

Abstract. Ocean acidification, caused by rising concentrations of carbon dioxide (CO2), is widely considered to be a major global threat to marine ecosystems. To investigate the potential effects of ocean acidification on the early life stages of a commercially important fish species, European sea bass (Dicentrarchus labrax), 12 000 larvae were incubated from hatch through metamorphosis under a matrix of two temperatures (17 and 19 °C) and two seawater pCO2s (400 and 750 μatm) and sampled regularly for 42 days. Calculated daily mortality was significantly affected by both temperature and pCO2, with both increased temperature and elevated pCO2 associated with lower daily mortality and a significant interaction between these two factors. There was no significant pCO2 effect noted on larval morphology during this period but larvae raised at 19 °C possessed significantly larger eyes and lower carbon:nitrogen ratios at the end of the study compared to those raised under 17 °C. These results suggest that D. labrax larvae are resilient to near-future oceanic conditions. However, when the incubation was continued to post-metamorphic (juvenile) animals (day 67–69), fish raised under a combination of 19 °C and 750 μatm pCO2 were significantly heavier and exhibited lower aerobic scopes than those incubated at 19 °C and 400 μatm. Most other studies investigating the effects of near-future oceanic conditions on the early life stages of marine fish have used incubations of relatively short durations and suggested these animals are resilient to ocean acidification. We propose the durations of these other studies may be insufficient for more subtle effects, such as those observed in this study, to become apparent. These findings may have important implications for both sea bass in a changing ocean and also for the interpretation of results from other studies that have shown resiliency in marine teleosts exposed to higher atmospheric concentrations of CO2.

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Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)

Journal of Nutritional Science ◽

10.1017/jns.2015.6 ◽

2015 ◽

Vol 4 ◽

Cited By ~ 13

Author(s):

Daniel Montero ◽

Genciana Terova ◽

Simona Rimoldi ◽

Lluis Tort ◽

Davinia Negrin ◽

...

Keyword(s):

Fatty Acids ◽

Regulatory Protein ◽

Dicentrarchus Labrax ◽

Head Kidney ◽

Sea Bass ◽

Dietary Fatty Acids ◽

Acth Stimulation ◽

European Sea Bass ◽

Stress Related Genes ◽

Steroidogenic Genes

AbstractDietary fatty acids have been shown to exert a clear effect on the stress response, modulating the release of cortisol. The role of fatty acids on the expression of steroidogenic genes has been described in mammals, but little is known in fish. The effect of different fatty acids on the release of cortisol and expression of stress-related genes of European sea bass (Dicentrarchus labrax) head kidney, induced by a pulse of adenocorticotrophin hormone (ACTH), was studied. Tissue was maintained in superfusion with 60 min of incubation with EPA, DHA, arachidonic acid (ARA), linoleic acid or α-linolenic acid (ALA) during 490 min. Cortisol was measured by RIA. The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real-time RT-PCR. There was an effect of the type of fatty acid on the ACTH-induced release of cortisol, values from ALA treatment being elevated within all of the experimental period. The expression of some steroidogenic genes, such as the steroidogenic acute regulatory protein (StAR) and c-fos, were affected by fatty acids, ALA increasing the expression of StAR after 1 h of ACTH stimulation whereas DHA, ARA and ALA increased the expression of c-fos after 20 min. ARA increased expression of the 11β-hydroxylase gene. Expression of heat shock protein 70 (HSP70) was increased in all the experimental treatments except for ARA. Results corroborate previous studies of the effect of different fatty acids on the release of cortisol in marine fish and demonstrate that those effects are mediated by alteration of the expression of steroidogenic genes.

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