The Scanning Electron Microscopy of Normal Human Peripheral Blood Lymphocytes

1976 ◽  
Vol 32 (3) ◽  
pp. 309-316 ◽  
Author(s):  
D. G. Newell ◽  
S. Roath ◽  
J. L. Smith
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Normal Human ◽  
Normal Human Peripheral Blood ◽  
Scanning Electron

scholarly journals Phytohemagglutinin-stimulated normal human peripheral blood lymphocytes in folate-depleted medium: an in vitro model for megaloblastic hemopoiesis

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 863-865 ◽  
Author(s):  
RJ Hayman ◽  
MB Van Der Weyden
Keyword(s):  
Peripheral Blood ◽  
Thymidine Incorporation ◽  
Human Peripheral Blood ◽  
Inhibitory Effect ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Normal Human ◽  
Normal Human Peripheral Blood

Abstract Normal human peripheral blood lymphocytes cultured with phytohemagglutinin in folate-free RPMI 1640 medium supplemented with normal human serum exhibit megaloblastic maturation. These changes are accompanied by a decrease in intracellular folate content, and when compared to folate-replete cells, reveal increased 3H-thymidine incorporation into DNA together with a decreased inhibitory effect of deoxyuridine on 3H-thymidine incorporation. This in vitro system of cellular folate deficiency is a convenient model for analysis of biochemical events accompanying megaloblastic maturation.

scholarly journals Phytohemagglutinin-stimulated normal human peripheral blood lymphocytes in folate-depleted medium: an in vitro model for megaloblastic hemopoiesis

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 863-865
Author(s):  
RJ Hayman ◽  
MB Van Der Weyden
Keyword(s):  
Peripheral Blood ◽  
Thymidine Incorporation ◽  
Human Peripheral Blood ◽  
Inhibitory Effect ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Normal Human ◽  
Normal Human Peripheral Blood

Normal human peripheral blood lymphocytes cultured with phytohemagglutinin in folate-free RPMI 1640 medium supplemented with normal human serum exhibit megaloblastic maturation. These changes are accompanied by a decrease in intracellular folate content, and when compared to folate-replete cells, reveal increased 3H-thymidine incorporation into DNA together with a decreased inhibitory effect of deoxyuridine on 3H-thymidine incorporation. This in vitro system of cellular folate deficiency is a convenient model for analysis of biochemical events accompanying megaloblastic maturation.

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