scholarly journals Phytohemagglutinin-stimulated normal human peripheral blood lymphocytes in folate-depleted medium: an in vitro model for megaloblastic hemopoiesis

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 863-865
Author(s):  
RJ Hayman ◽  
MB Van Der Weyden
Keyword(s):  
Peripheral Blood ◽  
Thymidine Incorporation ◽  
Human Peripheral Blood ◽  
Inhibitory Effect ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Normal Human ◽  
Normal Human Peripheral Blood

Normal human peripheral blood lymphocytes cultured with phytohemagglutinin in folate-free RPMI 1640 medium supplemented with normal human serum exhibit megaloblastic maturation. These changes are accompanied by a decrease in intracellular folate content, and when compared to folate-replete cells, reveal increased 3H-thymidine incorporation into DNA together with a decreased inhibitory effect of deoxyuridine on 3H-thymidine incorporation. This in vitro system of cellular folate deficiency is a convenient model for analysis of biochemical events accompanying megaloblastic maturation.

scholarly journals Phytohemagglutinin-stimulated normal human peripheral blood lymphocytes in folate-depleted medium: an in vitro model for megaloblastic hemopoiesis

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 863-865 ◽  
Author(s):  
RJ Hayman ◽  
MB Van Der Weyden
Keyword(s):  
Peripheral Blood ◽  
Thymidine Incorporation ◽  
Human Peripheral Blood ◽  
Inhibitory Effect ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Normal Human ◽  
Normal Human Peripheral Blood

Abstract Normal human peripheral blood lymphocytes cultured with phytohemagglutinin in folate-free RPMI 1640 medium supplemented with normal human serum exhibit megaloblastic maturation. These changes are accompanied by a decrease in intracellular folate content, and when compared to folate-replete cells, reveal increased 3H-thymidine incorporation into DNA together with a decreased inhibitory effect of deoxyuridine on 3H-thymidine incorporation. This in vitro system of cellular folate deficiency is a convenient model for analysis of biochemical events accompanying megaloblastic maturation.

Iodide enhances IgG synthesis by human peripheral blood lymphocytes in vitro

1983 ◽  
Vol 103 (2) ◽  
pp. 210-215 ◽  
Author(s):  
A. P. Weetman ◽  
A. M. McGregor ◽  
H. Campbell ◽  
J. H. Lazarus ◽  
H. K. Ibbertson ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Pokeweed Mitogen ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Igg Synthesis ◽  
Normal Human ◽  
Normal Human Peripheral Blood

Abstract. Several studies have suggested that iodide may increase thyroiditis and autoantibody synthesis. We have investigated the effect of iodide in vitro at physiologically relevant concentrations on immunoglobulin synthesis by normal human peripheral blood lymphocytes stimulated with pokeweed mitogen. Both the number of cells which synthesised IgG and the amount of IgG released into the culture supernatant increased significantly after culture in a medium with added iodide compared to a medium with added chloride. No effect of increasing concentrations of the added iodide from 10−3 mm to 10 mm was observed. These findings suggest that iodides may have a role in enhancing antibody synthesis which may be important when programmes of iodide supplementation are introduced into areas which are deficient.

Sign in / Sign up

Export Citation Format

Share Document