Germ-line transformation and RNAi of the ladybird beetle, Harmonia axyridis

2006 ◽  
Vol 15 (4) ◽  
pp. 507-512 ◽  
Author(s):  
H. Kuwayama ◽  
T. Yaginuma ◽  
O. Yamashita ◽  
T. Niimi
Keyword(s):  
Harmonia Axyridis ◽  
Germ Line ◽  
Ladybird Beetle ◽  
Germ Line Transformation

Probing Drosophila gene function by antisense RNA

Genome ◽  
1989 ◽  
Vol 31 (1) ◽  
pp. 422-425 ◽  
Author(s):  
Reinhard Schuh ◽  
Herbert Jäckle
Keyword(s):  
Antisense Rna ◽  
Germ Line ◽  
Conventional Technique ◽  
Transcription Unit ◽  
The Body ◽  
Mutant Phenotype ◽  
Drosophila Embryo ◽  
Alternative Approach ◽  
Pattern Forming ◽  
Germ Line Transformation

The conventional technique for assigning a particular genetic function to a cloned transcription unit has relied on the rescue of the mutant phenotype by germ line transformation. An alternative approach is to mimic a mutant phenotype by the use of antisense RNA injections to produce phenocopies. This approach has been successfully used to identify genes involved in early pattern forming processes in the Drosophila embryo. At the time when antisense RNA is injected, the embryo develops as a syncytium composed of about 5000 nuclei which share a common cytoplasm. The gene interactions required to establish the body plan occur before cellularization at the blastoderm stage. Thus the nuclei and their exported transcripts are accessible to the injected antisense RNA. The antisense RNA interferes with the endogenous RNA by an as yet unidentified mechanism. The extent of interference is only partial and produces phenocopies with characteristics of weak mutant alleles. In our lab and others, this approach has been successfully used to identify several genes required for normal Drosophila pattern formation.Key words: Drosophila segmentation, phenocopy, antisense RNA, Krüppel gene.

scholarly journals Plant male germ line transformation

1997 ◽  
Vol 12 (4) ◽  
pp. 949-956 ◽  
Author(s):  
A. Touraev ◽  
E. Stoger ◽  
V. Voronin ◽  
E. Heberle-Bors
Keyword(s):  
Germ Line ◽  
Male Germ Line ◽  
Germ Line Transformation

Transformation mapping of the regulatory elements of the ecdysone-inducible P1 gene of Drosophila melanogaster

1991 ◽  
Vol 11 (5) ◽  
pp. 2913-2917
Author(s):  
F Maschat ◽  
M L Dubertret ◽  
J A Lepesant
Keyword(s):  
Drosophila Melanogaster ◽  
Hormonal Regulation ◽  
Germ Line ◽  
Regulatory Elements ◽  
Fat Bodies ◽  
Germ Line Transformation ◽  
Third Instar Larvae

The transcription of the P1 gene is induced by 20-hydroxyecdysone in fat bodies of third-instar larvae. Germ line transformation showed that sequences between -138 to +276 contain elements required for a qualitatively correct developmental and hormonal regulation of P1 transcription. Sequences from -138 to -68 are essential for this expression.

Germ-line transformation of the Australian sheep blowflyLucilia cuprina

2002 ◽  
Vol 11 (1) ◽  
pp. 1-10 ◽  
Author(s):  
J. C. Heinrich ◽  
X. Li ◽  
R. A. Henry ◽  
N. Haack ◽  
L. Stringfellow ◽  
...  
Keyword(s):  
Germ Line ◽  
Australian Sheep ◽  
Germ Line Transformation
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