Genetic analysis of the bacteriocin-encoding plasmids pRJ6 and pRJ9 of Staphylococcus aureus by transposon mutagenesis and cloning of genes involved in bacteriocin production

1998 ◽  
Vol 85 (6) ◽  
pp. 972-984 ◽  
Author(s):  
S.S. Oliveira ◽  
J. dos Santos Nascimento ◽  
D.C. Póvoa ◽  
S. Amaral Araújo ◽  
M. Rodrigues Gamon ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Analysis ◽  
Transposon Mutagenesis ◽  
Bacteriocin Production

scholarly journals Genetic Characterization of Methicillin Resistant and Sensitive, Vancomycin Intermediate Staphylococcus aureus Strains Isolated from Different Iranian Hospitals

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Seyed Asghar Havaei ◽  
Amir Azimian ◽  
Hosein Fazeli ◽  
Mahmood Naderi ◽  
Kiarash Ghazvini ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Analysis ◽  
Clinical Samples ◽  
Different Types ◽  
Health Care Policies ◽  
Global Health Care ◽  
Vancomycin Resistant ◽  
Epidemiological Evaluation ◽  
Mrsa Strains

Background. Global concerns have been raised due to upward trend of Vancomycin Intermediate Staphylococcus aureus (VISA) and Vancomycin Resistant Staphylococcus aureus (VRSA) reports which mean casting doubt on the absolute effectiveness of the last line of antibiotic treatment for S. aureus, vancomycin. Hence, epidemiological evaluation can improve global health care policies. Methodology. 171 Isolates of Staphylococcus aureus were collected from different types of clinical samples in selected hospitals in Isfahan, Mashhad, and Tehran, Iran. Then, they were evaluated by agar screening, disk diffusion, and MIC method to determine their resistance to vancomycin and methicillin. The isolated VISA strains were then confirmed with genetic analysis by the evaluation of mecA and vanA genes, SCCmec, agr, and spa type, and also toxin profiles. MLST was also performed. Results and Conclusion. Our data indicated that 67% of isolated S. aureus strains were resistant to methicillin. Furthermore, five isolates (2.9%) had intermediate resistance to vancomycin (VISA). In contrast to usual association of VISA with MRSA strains, we found two isolates of MSSA-VISA. Therefore, our data suggests a probable parallel growing trend of VISA towards MSSA, along with MRSA strains. However, more samples are required to confirm these primarily data. Moreover, genetic analysis of the isolated VISA strains revealed that these strains are endemic Asian clones.

scholarly journals Whole Genome Sequencing and Complete Genetic Analysis Reveals Novel Pathways to Glycopeptide Resistance in Staphylococcus aureus

PLoS ONE ◽  
2011 ◽  
Vol 6 (6) ◽  
pp. e21577 ◽  
Author(s):  
Adriana Renzoni ◽  
Diego O. Andrey ◽  
Ambre Jousselin ◽  
Christine Barras ◽  
Antoinette Monod ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Analysis ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome ◽  
Glycopeptide Resistance

scholarly journals Genetic Analysis of a Vancomycin-Resistant Staphylococcus aureus Strain Isolated in Iran

mBio ◽  
2012 ◽  
Vol 3 (6) ◽  
Author(s):  
Amir Azimian ◽  
Seyed Asghar Havaei ◽  
Hosein Fazeli ◽  
Mahmood Naderi ◽  
Kiarash Ghazvini ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Analysis ◽  
Aureus Strain ◽  
Vancomycin Resistant

Growth conditions required for bacteriocin production by strains of Staphylococcus aureus

2004 ◽  
Vol 20 (9) ◽  
pp. 941-947 ◽  
Author(s):  
Jana�na dos Santos Nascimento ◽  
Jana�na Abrantes ◽  
Marcia Giambiagi-deMarval ◽  
Maria do Carmo de Freire Bastos
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Conditions ◽  
Bacteriocin Production

Biochemical Characterisation and Genetic Analysis of Aureocin A53, a New, Atypical Bacteriocin from Staphylococcus aureus

2002 ◽  
Vol 319 (3) ◽  
pp. 745-756 ◽  
Author(s):  
Daili Jacqueline Aguilar Netz ◽  
Regula Pohl ◽  
Annette G. Beck-Sickinger ◽  
Thorsten Selmer ◽  
Antonio J. Pierik ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Analysis ◽  
Biochemical Characterisation
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