THE DETERMINATION and STABILITY OF SODIUM NITROPRUSSIDE IN AQUEOUS SOLUTIONS (DETERMINATION and STABILITY OF SNP)

1977 ◽  
Vol 2 (2) ◽  
pp. 105-117 ◽  
Author(s):  
C.J. Vesey ◽  
G.A. Batistoni
Keyword(s):  
Aqueous Solutions ◽  
Sodium Nitroprusside

Is ‘NO’ news good news? Nitrogen oxides are not components of smoke that elicits germination in two smoke-stimulated species, Nicotiana attenuata and Emmenanthe penduliflora

2004 ◽  
Vol 14 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Catherine A. Preston ◽  
Romy Becker ◽  
Ian T. Baldwin
Keyword(s):  
Nitrogen Oxides ◽  
Aqueous Solutions ◽  
Sodium Nitroprusside ◽  
Great Basin ◽  
Good News ◽  
Wood Smoke ◽  
Nicotiana Attenuata ◽  
Great Basin Desert ◽  
No Donors ◽  
Germination Response

Both the California chaparral species,Emmenanthe pendulifloraBenth. (Hydrophyllaceae), and a tobacco native to the Great Basin Desert of south-western Utah,Nicotiana attenuataTorr. ex Wats. (Solanaceae), germinate in response to component(s) of wood smoke. Nitrogen oxides (NO and NO2), in amounts produced by a fire, have been proposed to be germination signals forE. penduliflora. We examined the germination response of dormant seeds ofE. pendulifloraandN. attenuatato aqueous solutions of smoke adjusted to different pHs, and two NO donors [sodium nitroprusside (SNP) andS-nitroso-N-acetylpenicillamine (SNAP)]. The smoke solutions, at pH 4 or 5, induced the maximum germination response. Aqueous solutions of SNP and SNAP, releasing NOx as high as 42 μM, had no effect on germination. Additionally, NO2–could not be detected in aqueous smoke extracts derived from combusted cellulose or wood. Therefore, unidentified cellulose combustion factors, rather than NOx, are likely to be the ecologically relevant germination signals.

Ultrathin frozen sections of yeast without aldehyde prefixation

1978 ◽  
Vol 36 (2) ◽  
pp. 58-59
Author(s):  
K. J. Böhm ◽  
a. E. Unger
Keyword(s):  
Aqueous Solutions ◽  
Biological Material ◽  
Yeast Cells ◽  
Frozen Sections ◽  
Good Preservation ◽  
Ultrathin Frozen Sections

During the last years it was shown that also by means of cryo-ultra-microtomy a good preservation of substructural details of biological material was possible. However the specimen generally was prefixed in these cases with aldehydes.Preparing ultrathin frozen sections of chemically non-prefixed material commonly was linked up to considerable technical and manual expense and the results were not always satisfying. Furthermore, it seems to be impossible to carry out cytochemical investigations by means of treating sections of unfixed biological material with aqueous solutions.We therefore tried to overcome these difficulties by preparing yeast cells (S. cerevisiae) in the following manner:

Imaging polymers, proteins and dna in aqueous solutions with the atomic force microscope

1989 ◽  
Vol 47 ◽  
pp. 32-33
Author(s):  
S.A.C. Gould ◽  
B. Drake ◽  
C.B. Prater ◽  
A.L. Weisenhorn ◽  
S.M. Lindsay ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Sequencing ◽  
Aqueous Solutions ◽  
Atomic Force Microscope ◽  
Piezoelectric Crystal ◽  
Atomic Force ◽  
Structure Of Molecules ◽  
Optical Lever

The atomic force microscope (AFM) is an instrument that can be used to image many samples of interest in biology and medicine. Images of polymerized amino acids, polyalanine and polyphenylalanine demonstrate the potential of the AFM for revealing the structure of molecules. Images of the protein fibrinogen which agree with TEM images demonstrate that the AFM can provide topographical data on larger molecules. Finally, images of DNA suggest the AFM may soon provide an easier and faster technique for DNA sequencing.The AFM consists of a microfabricated SiO2 triangular shaped cantilever with a diamond tip affixed at the elbow to act as a probe. The sample is mounted on a electronically driven piezoelectric crystal. It is then placed in contact with the tip and scanned. The topography of the surface causes minute deflections in the 100 μm long cantilever which are detected using an optical lever.

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