Improved method for transmission electron microscopy of ciliated cell monolayers maintained on gas-permeable membranes

1983 ◽  
Vol 132 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Michael G. Gabridge ◽  
Edward P. Dougherty
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ciliated Cell ◽  
Improved Method ◽  
Cell Monolayers ◽  
Transmission Electron

Changing width bandgap of TiO2 nanoparticles incorporating GO

MRS Advances ◽  
2019 ◽  
Vol 4 (61-62) ◽  
pp. 3423-3431
Author(s):  
Daniela K. Calvo-Ramos ◽  
Marina Vega-González ◽  
José Santos-Cruz ◽  
Francisco Javier De Moure-Flores ◽  
Sandra A. Mayén-Hernández
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Methylene Blue ◽  
Photocatalytic Activity ◽  
Graphene Oxide ◽  
Nir Spectroscopy ◽  
Improved Method ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron

ABSTRACTNanoparticles of titanium dioxide (TiO2), synthesized by the sonochemical technique, were mixed with different amounts of graphene oxide (GO), obtained by the improved method of Hummer, in order to modify their bandwidth. The TiO2/OG compounds were characterized using different techniques: X-ray Diffraction (XRD), transmission electron microscopy (TEM), Raman and UV-Vis-NIR spectroscopy. TiO2 bandgap decreased, with GO incorporation, from 3.2 to 2.72 eV when GO was present at 20 weigh percentage (TiO2/GO-20%). Photodegradation experiments of methylene blue (MB) were performed with the materials to verify their photocatalytic activity. At 40 minutes, the pure TiO2 degraded 48% of MB, whereas the compound TiO2/GO-20% degraded 88%, showing a good incorporation of both compounds and the improvement of TiO2 photocatalitic properties.

scholarly journals Embedding cell monolayers to investigate nanoparticle-plasmalemma interactions at transmission electron microscopy

2019 ◽  
Vol 63 (1) ◽  
Author(s):  
Manuela Costanzo ◽  
Manuela Malatesta
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Surface ◽  
Inexpensive Method ◽  
Biological Barrier ◽  
Cell Monolayers ◽  
Ultrastructural Evidence ◽  
Transmission Electron ◽  
Uptake Process ◽  
Intracellular Fate

Transmission electron microscopy is the technique of choice to visualize the spatial relationships between nanoconstructs and cells and especially to monitor the uptake process of nanomaterials. It is therefore crucial that the cell surface be preserved in its integrity, to obtain reliable ultrastructural evidence: the plasmalemma represents the biological barrier the nanomaterials have to cross, and the mode of membrane-nanoconstruct interaction is responsible for the intracellular fate of the nanomaterials. In this paper, we describe a simple and inexpensive method to process cell monolayers for ultrastructural morphology and immunocytochemistry, ensuring consistent preservation of the cell surface and of the occurring interactions with nanoparticles of different chemical composition.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

Sign in / Sign up

Export Citation Format

Share Document