Escherichia coli cells resistant to the DNA gyrase inhibitor, ciprofloxacin, overproduce a 60 kD protein homologous to GroEL

1990 ◽  
Vol 4 (3) ◽  
pp. 345-353 ◽  
Author(s):  
P. Hallett ◽  
A. Mehlert ◽  
A. Maxwell
Keyword(s):  
Escherichia Coli ◽  
Dna Gyrase ◽  
Escherichia Coli Cells

Antagonism of the B subunit of DNA gyrase eliminates plasmids pBR322 and pMG110 from Escherichia coli

1982 ◽  
Vol 152 (1) ◽  
pp. 338-344
Author(s):  
J S Wolfson ◽  
D C Hooper ◽  
M N Swartz ◽  
G L McHugh
Keyword(s):  
Escherichia Coli ◽  
Dna Gyrase ◽  
B Subunit ◽  
Plasmid Loss ◽  
Escherichia Coli Cells ◽  
Bacterial Mutant ◽  
Bacterial Enzyme ◽  
Kinetics Of ◽  
Plasmid Elimination ◽  
Native Plasmid

The constructed plasmid pBR322 and the native plasmid pMG110 were eliminated (cured) from growing Escherichia coli cells by the antagonism of the B subunit of the bacterial enzyme DNA gyrase. The antagonism may be by the growth of cells (i) at semipermissive temperatures in a bacterial mutant containing a thermolabile gyrase B subunit or (ii) at semipermissive concentrations of coumermycin A1, an antibiotic that specifically inhibits the B subunit of DNA gyrase. The kinetics of plasmid elimination indicate that plasmid loss occurs too rapidly to be explained solely by the faster growth of that plasmid-free bacteria and, therefore, represents interference with plasmid maintenance.

Expression of the F plasmid ccd toxin–antitoxin system in Escherichia coli cells under nutritional stress

2006 ◽  
Vol 52 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Marisela Aguirre-Ramírez ◽  
Jesús Ramírez-Santos ◽  
Laurence Van Melderen ◽  
M Carmen Gómez-Eichelmann
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Stationary Phase ◽  
Plasmid Stability ◽  
Dna Gyrase ◽  
Nutritional Stress ◽  
Adaptive Mutation ◽  
F Plasmid ◽  
Adaptive Mutations ◽  
Escherichia Coli Cells

The ccd system of the F plasmid encodes CcdB, a protein toxic to DNA-gyrase, and CcdA, its antitoxin. The function attributed to this system is to contribute to plasmid stability by killing bacteria that lose the plasmid during cell division. However, the function of ccd in resting bacteria is not clear. Results presented show that ccd transcription increases as bacteria enter stationary phase and that the amount of the Ccd proteins is higher in bacteria under nutritional stress than in growing bacteria. Moreover, an increase in the frequency of Lac+ "adaptive" mutations was observed in stationary-phase bacteria that over-express the Ccd proteins.Key words: ccd system, nutritional stress, adaptive mutation.

DETECTION OF GAMMA RADIATION EFFECT INDUCED BY COBELT-60 ON ESCHERICHIA COLI CELLS

2010 ◽  
Vol 13 (3) ◽  
pp. 129-133
Author(s):  
Ghusoon Ali Abdul Hasan Al-Sudany ◽  
◽  
Wasan Zuheir Majeed ◽  
Hind Jabbar Abdul Rhman Akram Al-Aubeidi ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gamma Radiation ◽  
Radiation Effect ◽  
Escherichia Coli Cells

scholarly journals AES and ToF-SIMS combination for single cell chemical imaging of gold nanoparticle-labeled Escherichia coli

2021 ◽  
Author(s):  
Cecile COURREGES ◽  
Mélanie Bonnecaze ◽  
Delphine Flahaut ◽  
Sophie Nolivos ◽  
Regis Grimaud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Single Cell ◽  
Electron Spectroscopy ◽  
Auger Electron ◽  
Chemical Imaging ◽  
Single Cell Level ◽  
Cell Level ◽  
Tof Sims ◽  
Escherichia Coli Cells

A chemical fingerprint of Escherichia coli cells surface labeled by gelatin coated gold nanoparticles was obtained by combining Auger Electron Spectroscopy (AES) for single cell level chemical images, and Time-of-Flight...

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