Notes An inversion truncating the creA gene of Aspergillus niduians results in carbon catabolite derepression

1990 ◽  
Vol 4 (5) ◽  
pp. 851-854 ◽  
Author(s):  
H. N. Arst ◽  
D. Tollervey ◽  
C. E. A. Dowzer ◽  
J. M. Kelly
Keyword(s):  
Carbon Catabolite Derepression ◽  
Crea Gene

scholarly journals Divergent degeneration of creA antitoxin genes from minimal CRISPRs and the convergent strategy of tRNA-sequestering CreT toxins

2021 ◽  
Vol 49 (18) ◽  
pp. 10677-10688
Author(s):  
Feiyue Cheng ◽  
Rui Wang ◽  
Haiying Yu ◽  
Chao Liu ◽  
Jun Yang ◽  
...  
Keyword(s):  
Adaptive Immunity ◽  
Deep Sequencing ◽  
Small Rnas ◽  
Transfer Rna ◽  
Open Reading Frame ◽  
Type I ◽  
Essential Information ◽  
Reading Frame ◽  
Crea Gene

Abstract Aside from providing adaptive immunity, type I CRISPR-Cas was recently unearthed to employ a noncanonical RNA guide (CreA) to transcriptionally repress an RNA toxin (CreT). Here, we report that, for most archaeal and bacterial CreTA modules, the creA gene actually carries two flanking ‘CRISPR repeats’, which are, however, highly divergent and degenerated. By deep sequencing, we show that the two repeats give rise to an 8-nt 5′ handle and a 22-nt 3′ handle, respectively, i.e., the conserved elements of a canonical CRISPR RNA, indicating they both retained critical nucleotides for Cas6 processing during divergent degeneration. We also uncovered a minimal CreT toxin that sequesters the rare transfer RNA for isoleucine, tRNAIleCAU, with a six-codon open reading frame containing two consecutive AUA codons. To fully relieve its toxicity, both tRNAIleCAU overexpression and supply of extra agmatine (modifies the wobble base of tRNAIleCAU to decipher AUA codons) are required. By replacing AUA to AGA/AGG codons, we reprogrammed this toxin to sequester rare arginine tRNAs. These data provide essential information on CreTA origin and for future CreTA prediction, and enrich the knowledge of tRNA-sequestering small RNAs that are employed by CRISPR-Cas to get addictive to the host.

scholarly journals Antisense Silencing of the creA Gene inAspergillus nidulans

2000 ◽  
Vol 66 (10) ◽  
pp. 4579-4581 ◽  
Author(s):  
L. Fernando Bautista ◽  
Alexei Aleksenko ◽  
Morten Hentzer ◽  
Anne Santerre-Henriksen ◽  
Jens Nielsen
Keyword(s):  
Aspergillus Nidulans ◽  
Growth Parameters ◽  
Colony Morphology ◽  
Gene Encoding ◽  
Antisense Expression ◽  
Catabolite Repressor ◽  
Crea Gene

ABSTRACT Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approximately one-half of that achieved in a null creA mutant. Unlike results for that mutant, however, growth parameters and colony morphology in the antisense transformants were not affected.

scholarly journals Proteolysis of Hexokinase PII is Not the Triggering Signal of Carbon Catabolite Derepression in Saccharomyces cerevisiae

Microbiology ◽  
1987 ◽  
Vol 133 (9) ◽  
pp. 2509-2516 ◽  
Author(s):  
M. T. FERNANDEZ ◽  
P. HERRERO ◽  
Y. S. LOPEZ-BOADO ◽  
R. FERNANDEZ ◽  
F. MORENO
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Catabolite Derepression

Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans

1991 ◽  
Vol 11 (11) ◽  
pp. 5701-5709 ◽  
Author(s):  
C E Dowzer ◽  
J M Kelly
Keyword(s):  
Aspergillus Nidulans ◽  
Zinc Finger ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Wilms Tumor ◽  
Yeast Cells ◽  
Cdna Clones ◽  
Crea Gene ◽  
Zinc Finger Region

The complete nucleotide sequence derived from a genomic clone and two cDNA clones of the creA gene of Aspergillus nidulans is presented. The gene contains no introns. The derived polypeptide of 415 amino acids contains two zinc fingers of the C2H2 class, frequent S(T)PXX motifs, and an alanine-rich region indicative of a DNA-binding repressor protein. The amino acid sequence of the zinc finger region has 84% similarity to the zinc finger region of Mig1, a protein involved in carbon catabolite repression in yeast cells, and it is related both to the mammalian Egr1 and Egr2 proteins and to the Wilms' tumor protein. A deletion removing the creA gene was obtained, by using in vitro techniques, in both a heterokaryon and a diploid strain but was unobtainable in a pure haploid condition. Evidence is presented suggesting that the phenotype of such a deletion, when not complemented by another creA allele, is leaky lethality allowing limited germination of the spore but not colony formation. This phenotype is far more extreme than that of any of the in vivo-generated mutations, and thus either the gene product may have an activator activity as well as a repressor function or some residual repressor function may be required for full viability.

scholarly journals Antagonism ofPythiumblight of zucchini byHypocrea jecorinadoes not require cellulase gene expression but is improved by carbon catabolite derepression

2006 ◽  
Vol 257 (1) ◽  
pp. 145-151 ◽  
Author(s):  
Verena Seidl ◽  
Monika Schmoll ◽  
Barbara Scherm ◽  
Virgilio Balmas ◽  
Bernhard Seiboth ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cellulase Gene ◽  
Carbon Catabolite Derepression

scholarly journals Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans.

1991 ◽  
Vol 11 (11) ◽  
pp. 5701-5709 ◽  
Author(s):  
C E Dowzer ◽  
J M Kelly
Keyword(s):  
Aspergillus Nidulans ◽  
Zinc Finger ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Wilms Tumor ◽  
Yeast Cells ◽  
Cdna Clones ◽  
Crea Gene ◽  
Zinc Finger Region

The complete nucleotide sequence derived from a genomic clone and two cDNA clones of the creA gene of Aspergillus nidulans is presented. The gene contains no introns. The derived polypeptide of 415 amino acids contains two zinc fingers of the C2H2 class, frequent S(T)PXX motifs, and an alanine-rich region indicative of a DNA-binding repressor protein. The amino acid sequence of the zinc finger region has 84% similarity to the zinc finger region of Mig1, a protein involved in carbon catabolite repression in yeast cells, and it is related both to the mammalian Egr1 and Egr2 proteins and to the Wilms' tumor protein. A deletion removing the creA gene was obtained, by using in vitro techniques, in both a heterokaryon and a diploid strain but was unobtainable in a pure haploid condition. Evidence is presented suggesting that the phenotype of such a deletion, when not complemented by another creA allele, is leaky lethality allowing limited germination of the spore but not colony formation. This phenotype is far more extreme than that of any of the in vivo-generated mutations, and thus either the gene product may have an activator activity as well as a repressor function or some residual repressor function may be required for full viability.

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