DNA fingerprinting of Eikenella cormdens by pulsed-field gel electrophoresis

1994 ◽  
Vol 9 (2) ◽  
pp. 95-98 ◽  
Author(s):  
L. Steffens ◽  
S. Franke ◽  
S. Nickel ◽  
A. Schwarzkopf ◽  
T. F. Flemmig ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Serotype 19F Multiresistant Pneumococcal Clone Harboring Two Erythromycin Resistance Determinants [erm(B) and mef(A)] in South Africa

2001 ◽  
Vol 45 (5) ◽  
pp. 1595-1598 ◽  
Author(s):  
Lesley McGee ◽  
Keith P. Klugman ◽  
Avril Wasas ◽  
Thora Capper ◽  
Adrian Brink
Keyword(s):  
South Africa ◽  
Streptococcus Pneumoniae ◽  
Private Sector ◽  
Dna Fingerprinting ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Erythromycin Resistance ◽  
Pulsed Field ◽  
Resistance Determinants ◽  
Genes Encoding

ABSTRACT One hundred eighteen erythromycin-resistant Streptococcus pneumoniae (ERSP) strains (MICs of ≥0.5 μg/ml) from five laboratories serving the private sector in South Africa were analyzed for the genes encoding resistance to macrolides. Sixty-seven ERSP strains (56.8%) contained the erm(B) gene, and 15 isolates (12.7%) contained the mef(A) gene. Thirty-six isolates (30.5%) harbored both the erm(B) and mef(A) genes and were highly resistant to erythromycin and clindamycin. DNA fingerprinting by BOX-PCR and pulsed-field gel electrophoresis identified 83% of these strains as belonging to a single multiresistant serotype 19F clone.

Sign in / Sign up

Export Citation Format

Share Document