ATPase activity associated with vacuoles and tonoplast vesicles isolated from the CAM plant, Kalanchoe daigremontiana

1984 ◽  
Vol 60 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Kunio Aoki ◽  
Kojiro Nishida
Keyword(s):  
Atpase Activity ◽  
Cam Plant ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

scholarly journals The Effect of Plant Extracts Kalanchoe daigremontiana and Aloe arborescens on the Metabolism of Human Multiple Myeloma Cells

2021 ◽  
Vol 11 (5) ◽  
pp. 13171-13186
Keyword(s):  
Multiple Myeloma ◽  
Atpase Activity ◽  
Tumor Cells ◽  
Plant Extracts ◽  
Glutathione Level ◽  
Myeloma Cells ◽  
Human Multiple Myeloma ◽  
Aloe Arborescens ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

The effect of plant extracts Kalanchoe daigremontiana, and Aloe arborescens on human multiple myeloma cells' viability was investigated. It was shown that plant extracts of kalanchoe and aloe reduced tumor cells' viability by 13 and 46%, respectively. The combination of plant extracts with doxorubicin showed an additive synergism of action, enhancing the antitumor effect. Treatment of multiple myeloma cells with plant extracts led to a decrease in intracellular reduced glutathione level. The intracellular glutathione level decreased by 25% under the action of kalanchoe extract and by 63% under the action of aloe extract. Extracts from kalanchoe and aloe decreased mitochondrial membrane potential by 19 and 53%, respectively. The results of the study showed that kalanchoe extract increased ATPase activity, but aloe extract did not affect the level of ATPase activity. The results showed that plant extracts of kalanchoe and aloe affect tumor cells' metabolism and contribute to their death. It was concluded that herbal extracts Kalanchoe daigremontiana and Aloe arborescens have antitumor activity, and aloe extract is more effective than kalanchoe.

Kinetic Properties of Phosphorylase and 6-Phosphofructokinase of Kalanchoe daigremontiana and Atriplex spongiosa

1975 ◽  
Vol 2 (3) ◽  
pp. 403 ◽  
Author(s):  
G Sutton
Keyword(s):  
Carbohydrate Metabolism ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Kinetic Properties ◽  
C4 Plant ◽  
Cam Plants ◽  
Regulation Of Carbohydrate Metabolism ◽  
Cam Plant ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

The kinetic properties of phosphorylase (EC 2.4.1.1) and 6-phosphofructokinase (EC 2.7.1.11) extracted from a crassulacean acid metabolism (CAM) plant, Kalanchoe daigremontiana Hamet et Perrier, and a C4 plant, Atriplex spongiosa F. Muell., were compared. The phosphorylase from the CAM plant was strongly inhibited by P1 (1 mM), phosphoenolpyruvate (PEP) (2 mM) and glucose (4 mM). The C4 phosphorylase was less strongly inhibited by P1, and not at all by PEP or glucose. The C4 6-phosphofructokinase was, at Km levels of substrate, about 100 times more sensitive to inhibition by PEP than the CAM enzyme. These results are discussed as the basis for a biochemical regulation of carbohydrate metabolism in CAM plants at night.

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