scholarly journals Determination of the Complete Amino-Acid Sequence of Protein S6 from the Wild-Type and a Mutant of Escherichia coli

1977 ◽  
Vol 75 (2) ◽  
pp. 497-512 ◽  
Author(s):  
Hans HITZ ◽  
Doris SCHAFER ◽  
Brigitte WITTMANN-LIEBOLD
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Wild Type ◽  
Complete Amino Acid Sequence

Determination of the Complete Amino Acid Sequence of Recombinant Human γ-Interferon Produced in Escherichia coli

1986 ◽  
Vol 6 (4) ◽  
pp. 331-336 ◽  
Author(s):  
SHOJIRO YAMAZAKI ◽  
TSUNEO SHIMAZU ◽  
SHIGENOBU KIMURA ◽  
HIROHIKO SHIMIZU
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Amino Acid Sequence ◽  
Γ Interferon

Determination of the Complete Amino Acid Sequence of Protein S21 from Escherichia coli Ribosomes

1975 ◽  
Vol 356 (2) ◽  
pp. 1955-1976 ◽  
Author(s):  
Joël Vandekerckhove ◽  
Wilfried Rombauts ◽  
Ben Peeters ◽  
Brigitte Wittmann-Liebold
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Amino Acid Sequence

scholarly journals Expression in Escherichia coli and characterization of a reconstituted recombinant 7Fe ferredoxin from Desulfovibrio africanus

1996 ◽  
Vol 314 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Johanneke L. H. BUSCH ◽  
Jacques L. J. BRETON ◽  
Barry M. BARTLETT ◽  
Richard JAMES ◽  
E. Claude HATCHIKIAN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Magnetic Circular Dichroism ◽  
Wild Type ◽  
E Coli ◽  
Excess Iron ◽  
Expression In Escherichia Coli ◽  
Type D

Desulfovibrio africanus ferredoxin III is a monomeric protein (molecular mass of 6585 Da) that contains one [3Fe-4S]1+/0 and one [4Fe-4S]2+/1+ cluster when isolated aerobically. The amino acid sequence consists of 61 amino acids, including seven cysteine residues that are all involved in co-ordination to the clusters. In order to isolate larger quantities of D. africanus ferredoxin III, we have overexpressed it in Escherichia coli by constructing a synthetic gene based on the amino acid sequence of the native protein. The recombinant ferredoxin was expressed in E. coli as an apoprotein. We have reconstituted the holoprotein by incubating the apoprotein with excess iron and sulphide in the presence of a reducing agent. The reconstituted recombinant ferredoxin appeared to have a lower stability than that of wild-type D. africanus ferredoxin III. We have shown by low-temperature magnetic circular dichroism and EPR spectroscopy that the recombinant ferredoxin contains a [3Fe-4S]1+/0 and a [4Fe-4S]2+/1+ cluster similar to those found in native D. africanus ferredoxin III. These results indicate that the two clusters have been correctly inserted into the recombinant ferredoxin.

scholarly journals DETERMINATION OF THE COMPLETE AMINO-ACID SEQUENCE OF PORCINE MINIPLASMINOGEN

1985 ◽  
Vol 149 (2) ◽  
pp. 283-285
Author(s):  
Thomas Marti ◽  
Johann Schaller ◽  
Egon E. Rickli
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Amino Acid Sequence

scholarly journals DETERMINATION OF THE COMPLETE AMINO ACID SEQUENCE OF CALMODULIN (PHENYLALANINE-RICH ACIDIC PROTEIN II) FROM BOVINE BRAIN

1980 ◽  
Vol 1 (3) ◽  
pp. 248-264 ◽  
Author(s):  
HISATAKA KASAI ◽  
YUKIO KATO ◽  
TOSHIAKI ISOBE ◽  
HIROSHI KAWASAKI ◽  
TSUNEO OKUYAMA
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Acidic Protein ◽  
Complete Amino Acid Sequence

scholarly journals Human heart cytochrome c oxidase subunit VIII Purification and determination of the complete amino acid sequence

FEBS Letters ◽  
1988 ◽  
Vol 240 (1-2) ◽  
pp. 127-132 ◽  
Author(s):  
André B.P. Van Kuilenburg ◽  
Anton O. Muijsers ◽  
Hans Demol ◽  
Henk L. Dekker ◽  
Jozef J. Van Beeumen
Keyword(s):  
Amino Acid ◽  
Cytochrome C ◽  
Amino Acid Sequence ◽  
Cytochrome C Oxidase ◽  
Human Heart ◽  
Complete Amino Acid Sequence ◽  
Oxidase Subunit
Sign in / Sign up

Export Citation Format

Share Document