Complete Amino Acid Sequence Determination of Rat Epidermal Growth Factor:Characterization of a Truncated Form with Full In Vitro Biological Activity

Proteins ◽  
1987 ◽  
pp. 37-44
Author(s):  
Edouard C. Nice ◽  
John A. Smith ◽  
Robert L. Moritz ◽  
Michael J. O’Hare ◽  
Philip S. Rudland ◽  
...  
Keyword(s):  
Biological Activity ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Sequence Determination ◽  
Truncated Form ◽  
Complete Amino Acid Sequence ◽  
Epidermal Growth

Complete Amino Acid Sequence Determination of the Major Allergen of Peach (Prunus persica) Pru p1

1999 ◽  
Vol 380 (11) ◽  
Author(s):  
Elide A. Pastorello ◽  
Claudio Ortolani ◽  
Chiara Baroglio ◽  
Valerio Pravettoni ◽  
Marco Ispano ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Prunus Persica ◽  
Major Allergen ◽  
Sequence Determination ◽  
Complete Amino Acid Sequence

Determination of the Complete Amino Acid Sequence of Recombinant Human γ-Interferon Produced in Escherichia coli

1986 ◽  
Vol 6 (4) ◽  
pp. 331-336 ◽  
Author(s):  
SHOJIRO YAMAZAKI ◽  
TSUNEO SHIMAZU ◽  
SHIGENOBU KIMURA ◽  
HIROHIKO SHIMIZU
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Amino Acid Sequence ◽  
Γ Interferon

scholarly journals Structural characterization and in vitro lipid binding studies of non-specific lipid transfer protein 1 (nsLTP1) from fennel (Foeniculum vulgare) seeds

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mekdes Megeressa ◽  
Bushra Siraj ◽  
Shamshad Zarina ◽  
Aftab Ahmed
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
3D Structure ◽  
Lipid Binding ◽  
Lipid Transfer ◽  
Binding Studies ◽  
Foeniculum Vulgare ◽  
Complete Amino Acid Sequence ◽  
Specific Lipid

AbstractNon-specific lipid transfer proteins (nsLTPs) are cationic proteins involved in intracellular lipid shuttling in growth and reproduction, as well as in defense against pathogenic microbes. Even though the primary and spatial structures of some nsLTPs from different plants indicate their similar features, they exhibit distinct lipid-binding specificities signifying their various biological roles that dictate further structural study. The present study determined the complete amino acid sequence, in silico 3D structure modeling, and the antiproliferative activity of nsLTP1 from fennel (Foeniculum vulgare) seeds. Fennel is a member of the family Umbelliferae (Apiaceae) native to southern Europe and the Mediterranean region. It is used as a spice medicine and fresh vegetable. Fennel nsLTP1 was purified using the combination of gel filtration and reverse-phase high-performance liquid chromatography (RP-HPLC). Its homogeneity was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. The purified nsLTP1 was treated with 4-vinyl pyridine, and the modified protein was then digested with trypsin. The complete amino acid sequence of nsLTP1 established by intact protein sequence up to 28 residues, overlapping tryptic peptides, and cyanogen bromide (CNBr) peptides. Hence, it is confirmed that fennel nsLTP1 is a 9433 Da single polypeptide chain consisting of 91 amino acids with eight conserved cysteines. Moreover, the 3D structure is predicted to have four α-helices interlinked by three loops and a long C-terminal tail. The lipid-binding property of fennel nsLTP1 is examined in vitro using fluorescent 2-p-toluidinonaphthalene-6-sulfonate (TNS) and validated using a molecular docking study with AutoDock Vina. Both of the binding studies confirmed the order of binding efficiency among the four studied fatty acids linoleic acid > linolenic acid > Stearic acid > Palmitic acid. A preliminary screening of fennel nsLTP1 suppressed the growth of MCF-7 human breast cancer cells in a dose-dependent manner with an IC50 value of 6.98 µM after 48 h treatment.

Partial amino acid sequence determination of Bovine Corneal Protein 54 K (BCP 54)

1990 ◽  
Vol 9 (8) ◽  
pp. 781-786 ◽  
Author(s):  
David L. Cooper ◽  
Edward W. Baptist ◽  
Jan Enghild ◽  
Howard Lee ◽  
Narayana Isola ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Sequence Determination ◽  
Partial Amino Acid Sequence

Complete Amino Acid Sequence of Globin Chains and Biological Activity of Fragmented Crocodile Hemoglobin (Crocodylus siamensis)

2012 ◽  
Vol 31 (6) ◽  
pp. 466-476 ◽  
Author(s):  
Saowaluck Srihongthong ◽  
Anawat Pakdeesuwan ◽  
Sakda Daduang ◽  
Tomohiro Araki ◽  
Apisak Dhiravisit ◽  
...  
Keyword(s):  
Biological Activity ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Amino Acid Sequence ◽  
Globin Chains ◽  
Crocodylus Siamensis
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