scholarly journals Inter-Relationships between Proton Electrochemical Gradient, Adenine-Nucleotide Phosphorylation Potential and Respiration, during Substrate-Level and Oxidative Phosphorylation by Mitochondria from Brown Adipose Tissue of Cold-Adapted Guinea-Pigs

1977 ◽  
Vol 75 (2) ◽  
pp. 601-612 ◽  
Author(s):  
David G. NICHOLLS ◽  
Vibeke S. M. BERNSON
Keyword(s):  
Adipose Tissue ◽  
Oxidative Phosphorylation ◽  
Brown Adipose Tissue ◽  
Guinea Pigs ◽  
Adenine Nucleotide ◽  
Electrochemical Gradient ◽  
Cold Adapted ◽  
Phosphorylation Potential ◽  
Brown Adipose ◽  
Substrate Level

Regulation of Brown Adipose Tissue Function in Cold-Adapted Animals: Lack of ß3-Adrenergic Receptors in Brown Adipose Tissue of Cold-Adapted Guinea Pigs

2019 ◽  
pp. 331-343
Author(s):  
Jean Himms-Hagen ◽  
Nicole Bégin-Heick ◽  
Anna-Lisa Kates ◽  
Joan Triandafillou ◽  
Masoud Ghorbani ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Guinea Pigs ◽  
Adrenergic Receptors ◽  
Cold Adapted ◽  
Brown Adipose

The Effects of Thyroxine and Isopropylnoradrenaline on Cytochrome Oxidase Activity in Brown Adipose Tissue

1973 ◽  
Vol 51 (10) ◽  
pp. 751-758 ◽  
Author(s):  
H. M. C. Heick ◽  
C. Vachon ◽  
Mary Ann Kallai ◽  
Nicole Bégin-Heick ◽  
J. LeBlanc
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Protein Content ◽  
Cytochrome Oxidase ◽  
Cold Adaptation ◽  
Metabolic Response ◽  
Mitochondrial Enzymes ◽  
Cold Adapted ◽  
Brown Adipose ◽  
Hormonal Treatments

Groups of animals were treated with injections of isopropylnoradrenaline, thyroxine, or both hormones together. The effects of these hormonal treatments on the size, protein content, and level of some mitochondrial enzymes, in particular the cytochrome oxidase, were determined and compared to the effect on these parameters produced by cold adaptation. The changes observed were correlated with the resistance of the animals to cold stress and with their metabolic response to injections of isopropylnoradrenaline. All treatments increased the size of the brown adipose tissue. Whereas thyroxine had little effect on the protein content and cytochrome oxidase, both isopropylnoradrenaline and cold adaptation produced increases in these parameters. It appears that the isopropylnoradrenaline-treated animals mimic more closely the cold-adapted animals than do those with thyroxine treatment. However, the isopropylnoradrenaline-treated animals are not as resistant to cold as the cold-adapted animals.

scholarly journals Oxidative phosphorylation. The specific binding of trimethyltin and triethyltin to rat liver mitochondria

1970 ◽  
Vol 118 (1) ◽  
pp. 171-179 ◽  
Author(s):  
W. N. Aldridge ◽  
B. W. Street
Keyword(s):  
Adipose Tissue ◽  
Oxidative Phosphorylation ◽  
Brown Adipose Tissue ◽  
Binding Site ◽  
Rat Liver ◽  
Specific Binding ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Affinity Constants ◽  
Brown Adipose

1. The binding of trimethyltin and triethyltin to rat liver mitochondria was determined and the results were analysed by the method of Scatchard (1949). 2. One binding site (site 1) has the correct characteristics for the site to which trimethyltin and triethyltin are attached when they inhibit oxidative phosphorylation. For each compound the concentration of site 1 is 0.8nmol/mg of protein and the ratios of their affinity constants are the same as the ratio of the concentrations inhibiting oxidative phosphorylation. 3. Binding site 1 is present in a fraction derived from mitochondria containing only 15% of the original protein. In this preparation ultrasonication rapidly destroyed site 1. 4. Dimethyltin and diethyltin do not prevent binding of triethyltin to rat liver mitochondria, whereas triethyl-lead does. 5. Trimethyltin and triethyltin bind to mitochondria from brown adipose tissue and the results indicate a binding site 1 similar to that in rat liver mitochondria. 6. The advantages and limitations of this approach to the study of inhibitors are discussed.

An online guided e-journal exercise in pre-clerkship years: Oxidative phosphorylation in brown adipose tissue

2014 ◽  
Vol 42 (3) ◽  
pp. 259-269 ◽  
Author(s):  
Emine Ercikan Abali ◽  
Sangita Phadtare ◽  
Jim Galt ◽  
Barbara Brodsky
Keyword(s):  
Adipose Tissue ◽  
Oxidative Phosphorylation ◽  
Brown Adipose Tissue ◽  
Brown Adipose
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