Further Evidence for the Structure of the Teichoic Acids from Bacillus stearothermophilus B65 and Bacillus subtilis var. niger WM

1978 ◽  
Vol 85 (2) ◽  
pp. 433-436 ◽  
Author(s):  
Willem R. BOER ◽  
Jan T. M. WOUTERS ◽  
Alistair J. ANDERSON ◽  
A. Ronald ARCHIBALD
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Stearothermophilus ◽  
Teichoic Acids

scholarly journals In Vivo and In Vitro evaluation of the efficacy of a peracetic acid-based disinfectant for decontamination of acrylic resins

2006 ◽  
Vol 17 (2) ◽  
pp. 117-121 ◽  
Author(s):  
Ana Lúcia Campani Chassot ◽  
Maria Inês Pereira Poisl ◽  
Susana Maria Werner Samuel
Keyword(s):  
Bacillus Subtilis ◽  
Peracetic Acid ◽  
Bacillus Stearothermophilus ◽  
Acrylic Resin ◽  
Human Saliva ◽  
Acrylic Resins ◽  
The Media ◽  
High Level

The purpose of this study was to assess the antimicrobial efficacy of a peracetic acid-based disinfectant for decontamination of heat-polymerized, chemically activated and microwave-polymerized acrylic resins. Resin plates were contaminated in vivo upon intraoral use by 10 volunteers for 7 nights and slabs were contaminated in vitro by contact with Bacillus subtilis and Bacillus stearothermophilus. The contaminated acrylic resin specimens were immersed in a 0.2% peracetic acid-based disinfectant (Sterilife®; Lifemed) for 5 min or 10 min and placed in a BHI culture medium. After incubation at 37°C for 48 h, bacterial growth was assessed by analyzing turbidity of the medium. For all types of acrylic resin, no turbidity of the medium was observed for any of the resin specimens immersed in the peracetic acid-based disinfectant for either 5 or 10 min. On the other hand, the media with specimens that were not immersed in the disinfectant (control) showed turbidity in 100% of the cases, indicating the presence of microorganisms in both tested conditions. In conclusion, immersion for at least 5 min in a 0.2% peracetic acid-based disinfectant promoted high-level disinfection of heat-polymerized, chemically activated and microwave-polymerized acrylic resins contaminated with either human saliva or Bacillus subtilis or Bacillus stearothermophilus.

Teichoic Acids from Bacterial Walls: Structure of Teichoic Acid from the walls of Bacillus subtilis

Nature ◽  
1959 ◽  
Vol 184 (4682) ◽  
pp. 248-249 ◽  
Author(s):  
J. J. ARMSTRONG ◽  
J. BADDILEY ◽  
J. G. BUCHANAN
Keyword(s):  
Bacillus Subtilis ◽  
Teichoic Acid ◽  
Teichoic Acids

Evaluation of Methods Used to Detect Antibiotic Residues in Milk1

1981 ◽  
Vol 44 (9) ◽  
pp. 696-698 ◽  
Author(s):  
D. M. MACAULAY ◽  
V. S. PACKARD
Keyword(s):  
Bacillus Subtilis ◽  
False Positive ◽  
Bacillus Stearothermophilus ◽  
Assay Method ◽  
Antibiotic Residues ◽  
Positive Results ◽  
Evaluation Of Methods ◽  
Disc Assay

Over nine replicate trials, five methods for detecting antibiotic residues were evaluated for sensitivity to various levels of penicillin, erythromycin and chloramphenicol in milk. The methods included (a) the Charm test, (b) Delvotest P, (c) disc assay with Bacillus subtilis on whey agar, (d) B. subtilis on Antibiotic Medium #1 and (e) Difco disc assay method using Bacillus stearothermophilus var. calidolactis on PMI agar. Specific to penicillin only, the Charm test detected the antibiotic 100% of the time down to and including 0.01 unit/ml, dropping to 56% detectability at 0.0025 unit/ml. Delvotest P detected penicillin 100% of the time at 0.025 unit/ml, but at varying degrees of sensitivity through 0.0025 unit/ml, and with evidence of showing false-positive results. Methods (c), (d) and (e) detected penicillin at 0.05 unit/ml 78, 89 and 100% of the time, respectively. Methods (d) and (e) were generally more sensitive to erythromycin and chloramphenicol than either (b) or (c).

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