Nuclease hypersensitivity in the beta-globin gene region of K562 cells

The temporal order of replication of DNA sequences in the chromosomal domain containing the human beta-globin gene cluster and its flanking sequences (140 kilobases) was measured and compared in two different human cell lines. In human erythroleukemia (K562) cells, in which embryonic and fetal globin genes are transcribed, all of the sequences we examined from the beta-globin domain replicated early during S phase, while in HeLa cells, in which globin genes are transcriptionally silent, these sequences replicated late during S. Potential sites of initiation of DNA replication within this domain were identified. The beta-globin gene domain was also found to differ with respect to the nuclease sensitivity of the chromatin in these two cell lines. In K562 cells, hypersensitive sites for endogenous nucleases and DNase I were present in the chromatin near the earliest-replicating segments in the beta-globin domain.

Download Full-text

Erythroleukemia (K562) cells contain a functional beta-globin gene

Molecular and Cellular Biology ◽

10.1128/mcb.4.11.2553-2555.1984 ◽

1984 ◽

Vol 4 (11) ◽

pp. 2553-2555

Author(s):

M Donovan-Peluso ◽

K Young ◽

C Dobkin ◽

A Bank

Keyword(s):

Hela Cells ◽

Globin Gene ◽

K562 Cells ◽

Beta Globin ◽

Erythroid Cells ◽

Beta Globin Gene ◽

Rna Transcripts

K562 cells are human erythroid cells that synthesize embryonic and fetal globins but not adult beta-globin. A cloned beta-globin gene was isolated from K562 cells and transfected into HeLa cells. The RNA transcripts produced were comparable in both amount and size to those obtained with a normal beta-globin gene.

Download Full-text

Erythroleukemia (K562) cells contain a functional beta-globin gene.

Molecular and Cellular Biology ◽

10.1128/mcb.4.11.2553 ◽

1984 ◽

Vol 4 (11) ◽

pp. 2553-2555 ◽

Cited By ~ 10

Author(s):

M Donovan-Peluso ◽

K Young ◽

C Dobkin ◽

A Bank

Keyword(s):

Hela Cells ◽

Globin Gene ◽

K562 Cells ◽

Beta Globin ◽

Erythroid Cells ◽

Beta Globin Gene ◽

Rna Transcripts

K562 cells are human erythroid cells that synthesize embryonic and fetal globins but not adult beta-globin. A cloned beta-globin gene was isolated from K562 cells and transfected into HeLa cells. The RNA transcripts produced were comparable in both amount and size to those obtained with a normal beta-globin gene.

Download Full-text

The gamma-delta-beta-globin gene region in G gamma-beta +-hereditary persistence of fetal hemoglobin

Blood ◽

10.1182/blood.v59.4.828.bloodjournal594828 ◽

1982 ◽

Vol 59 (4) ◽

pp. 828-831

Author(s):

JF Balsley ◽

E Rappaport ◽

E Schwartz ◽

S Surrey

Keyword(s):

Globin Gene ◽

Blot Hybridization ◽

Fetal Hemoglobin ◽

Restriction Enzymes ◽

Restriction Endonuclease Analysis ◽

Gene Region ◽

Beta Globin ◽

Gamma Delta ◽

Beta Globin Gene ◽

Hereditary Persistence

We report restriction endonuclease analysis of the gamma-delta-beta- globin gene region in a mother and child heterozygous for G gamma-beta +-hereditary persistence of fetal hemoglobin (HPFH). The affected chromosome in these persons directs the production of G gamma-chains and beta-chains but not A gamma-chains. DNA was digested with several restriction enzymes and was examined for gamma, delta, beta sequences by blot hybridization. Only normal digestion fragments were present. By sensitive methods, we were unable to detect a deletion in the entire gamma-delta-beta-globin gene region of the affected chromosome, indicating that in this family, G gamma-beta +-HPFH is not due to a large deletion.

Download Full-text