Expression of Active Streptolysin O in Escherichia coli as a Maltose-Binding-Protein-Streptolysin-O Fusion Protein. The N-Terminal 70 Amino Acids are not Required for Hemolytic Activity

Ulrich Weller; Lutz Muller; Martina Messner; Michael Palmer; Angela Valeva; Jorgen Tranum-Jensen; Pushpa Agrawal; Christine Biermann; Andreas Dobereiner; Michael A. Kehoe; Sucharit Bhakdi

doi:10.1111/j.1432-1033.1996.00034.x

Expression of Active Streptolysin O in Escherichia coli as a Maltose-Binding-Protein-Streptolysin-O Fusion Protein. The N-Terminal 70 Amino Acids are not Required for Hemolytic Activity

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1996.00034.x ◽

1996 ◽

Vol 236 (1) ◽

pp. 34-39 ◽

Cited By ~ 43

Author(s):

Ulrich Weller ◽

Lutz Muller ◽

Martina Messner ◽

Michael Palmer ◽

Angela Valeva ◽

...

Keyword(s):

Escherichia Coli ◽

Amino Acids ◽

Fusion Protein ◽

Hemolytic Activity ◽

Binding Protein ◽

Maltose Binding Protein ◽

Streptolysin O

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Thirty-three amino acids of the mature moiety of an unprocessed maltose-binding protein are sufficient for export in Escherichia coli.

Journal of Bacteriology ◽

10.1128/jb.176.11.3397-3399.1994 ◽

1994 ◽

Vol 176 (11) ◽

pp. 3397-3399 ◽

Cited By ~ 3

Author(s):

G A Barkocy-Gallagher ◽

J G Cannon ◽

P J Bassford

Keyword(s):

Escherichia Coli ◽

Amino Acids ◽

Binding Protein ◽

Maltose Binding Protein

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Mathematical modelling of Sec pathway mechanism in Escherichia coli: a case study for periplasmic translocation of maltose binding protein–glucose isomerase fusion protein

Chemical Engineering Science ◽

10.1016/j.ces.2003.11.024 ◽

2004 ◽

Vol 59 (5) ◽

pp. 1027-1038

Author(s):

Berna Sarıyar ◽

Amable Hortaçsu

Keyword(s):

Escherichia Coli ◽

Fusion Protein ◽

Mathematical Modelling ◽

Binding Protein ◽

Glucose Isomerase ◽

Maltose Binding Protein ◽

Sec Pathway

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Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: Efficient regeneration of the fusion protein and selective cleavage with trypsin

Protein Science ◽

10.1002/pro.5560050307 ◽

1996 ◽

Vol 5 (3) ◽

pp. 456-467 ◽

Cited By ~ 25

Author(s):

Guo-Qiang Chen ◽

J. Eric Gouaux

Keyword(s):

Escherichia Coli ◽

Fusion Protein ◽

Binding Protein ◽

Maltose Binding Protein ◽

Water Soluble ◽

Efficient Regeneration

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Sublingual vaccination with fusion protein consisting of the functional domain of hemagglutinin A of Porphyromonas gingivalis and Escherichia coli maltose-binding protein elicits protective immunity in the oral cavity

FEMS Immunology & Medical Microbiology ◽

10.1111/j.1574-695x.2011.00895.x ◽

2011 ◽

Vol 64 (2) ◽

pp. 265-272 ◽

Cited By ~ 6

Author(s):

Satoshi Yuzawa ◽

Tomoko Kurita-Ochiai ◽

Tomomi Hashizume ◽

Ryoki Kobayashi ◽

Yoshimitsu Abiko ◽

...

Keyword(s):

Escherichia Coli ◽

Oral Cavity ◽

Fusion Protein ◽

Porphyromonas Gingivalis ◽

Protective Immunity ◽

Binding Protein ◽

Maltose Binding Protein ◽

Functional Domain

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Cloning, Expression and Purification of Full-Length Rep78 of Adeno-associated Virus as a Fusion Protein with Maltose Binding Protein in Escherichia coli

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1995.1396 ◽

1995 ◽

Vol 208 (2) ◽

pp. 714-720 ◽

Cited By ~ 7

Author(s):

R.B. Batchu ◽

D.A. Miles ◽

T.M. Rechtin ◽

R.R. Drake ◽

P.L. Hermonat

Keyword(s):

Escherichia Coli ◽

Fusion Protein ◽

Binding Protein ◽

Maltose Binding Protein ◽

Full Length ◽

Expression And Purification ◽

Adeno Associated Virus

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Intragenic reversion mutations that improve export of maltose-binding protein in Escherichia coli malE signal sequence mutants.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)35795-2 ◽

1986 ◽

Vol 261 (7) ◽

pp. 3389-3395

Author(s):

J P Ryan ◽

M C Duncan ◽

V A Bankaitis ◽

P J Bassford

Keyword(s):

Escherichia Coli ◽

Signal Sequence ◽

Binding Protein ◽

Maltose Binding Protein

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Production of MBP(maltose binding protein)-GroES fusion protein and utilization to stimulate GroEL-mediated protein refolding

Journal of Fermentation and Bioengineering ◽

10.1016/s0922-338x(97)80356-x ◽

1998 ◽

Vol 85 (1) ◽

pp. 69-73 ◽

Cited By ~ 2

Author(s):

Yoshiyuki Ishii ◽

Kaoru Murakami ◽

Hiroaki I.-Ogawa ◽

Akihiko Kondo ◽

Yasuhiko Kato

Keyword(s):

Fusion Protein ◽

Binding Protein ◽

Protein Refolding ◽

Maltose Binding Protein

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Active transport of maltose in Escherichia coli K12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)83799-7 ◽

1982 ◽

Vol 257 (10) ◽

pp. 5455-5461

Author(s):

H A Shuman

Keyword(s):

Escherichia Coli ◽

Active Transport ◽

Cytoplasmic Membrane ◽

Binding Protein ◽

Substrate Recognition ◽

Maltose Binding Protein ◽

Recognition Site ◽

Escherichia Coli K12

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Physical interaction between the phage lambda receptor protein and the carrier-immobilized maltose-binding protein of Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)68406-1 ◽

1981 ◽

Vol 256 (22) ◽

pp. 11385-11388

Author(s):

P. Bavoil ◽

H. Nikaido

Keyword(s):

Escherichia Coli ◽

Binding Protein ◽

Maltose Binding Protein ◽

Receptor Protein ◽

Physical Interaction ◽

Phage Lambda

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Synthesis of precursor maltose-binding protein with proline in the +1 position of the cleavage site interferes with the activity of Escherichia coli signal peptidase I in vivo.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)48419-0 ◽

1992 ◽

Vol 267 (2) ◽

pp. 1231-1238

Author(s):

G A Barkocy-Gallagher ◽

P J Bassford

Keyword(s):

Escherichia Coli ◽

Cleavage Site ◽

Binding Protein ◽

Maltose Binding Protein ◽

Signal Peptidase ◽

Signal Peptidase I

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